Gastric Juice MicroRNAs as Potential Biomarkers for Screening Gastric Cancer: A Systematic Review

Virgilio, Edoardo; Giarnieri, Enrico; Giovagnoli, Maria Rosaria; Montagnini, Monica; Proietti, A.; D’Urso, Rosaria; Mercantini, Paolo; Cavallini, Marco

doi:10.21873/anticanres.12265

Cited by 20 publications

(15 citation statements)

References 12 publications

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“…PVT1 might serve as a promising biomarker for early detection and prognosis prediction of GC[ 121 ]. Gastric juice miR-421, miR-21 , miR-106a and miR-129 represent a potential biomarker for screening GC [ 133 ].…”

Section: Stomach Specific Biomarkermentioning

confidence: 99%

Biomarkers of gastric cancer: Current topics and future perspective

Matsuoka

Yashiro

2018

WJG

375

266

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Gastric cancer (GC) is one of the most prevalent malignant types in the world and an aggressive disease with a poor 5-year survival. This cancer is biologically and genetically heterogeneous with a poorly understood carcinogenesis at the molecular level. Although the incidence is declining, the outcome of patients with GC remains dismal. Thus, the detection at an early stage utilizing useful screening approaches, selection of an appropriate treatment plan, and effective monitoring is pivotal to reduce GC mortalities. Identification of biomarkers in a basis of clinical information and comprehensive genome analysis could improve diagnosis, prognosis, prediction of recurrence and treatment response. This review summarized the current status and approaches in GC biomarker, which could be potentially used for early diagnosis, accurate prediction of therapeutic approaches and discussed the future perspective based on the molecular classification and profiling.

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Section: Stomach Specific Biomarkermentioning

confidence: 99%

Biomarkers of gastric cancer: Current topics and future perspective

Matsuoka

Yashiro

2018

WJG

375

266

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“…Dysregulation of miR-21 makes it a very good candidate as a biomarker for developing tools for the early diagnosis and prognosis of cancers. More importantly, as recent evidence suggests, miR-21 is not only present in tissues but also in body fluids, such as serum [ 52 , 53 ], saliva [ 54 , 55 ], plasma [ 46 , 56 , 57 ], gastric juice [ 58 , 59 ], sputum [ 60 , 61 ], and cerebrospinal fluid [ 62 , 63 , 64 ]. Therefore, by examining body fluids and the levels of miR-21, it may be possible to differentiate cancer patients from healthy individuals and detect cancer at early stages dependent on the diagnostic potential of miR-21 as a biomarker.…”

Section: Introductionmentioning

confidence: 99%

Micro RNA Sensing with Green Emitting Silver Nanoclusters

Yourston

Krasnoslobodtsev

2020

Molecules

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Micro RNA (miR) are regulatory non-coding RNA molecules, which contain a small number of nucleotides ~18–28 nt. There are many various miR sequences found in plants and animals that perform important functions in developmental, metabolic, and disease processes. miRs can bind to complementary sequences within mRNA molecules thus silencing mRNA. Other functions include cardiovascular and neural development, stem cell differentiation, apoptosis, and tumors. In tumors, some miRs can function as oncogenes, others as tumor suppressors. Levels of certain miR molecules reflect cellular events, both normal and pathological. Therefore, miR molecules can be used as biomarkers for disease diagnosis and prognosis. One of these promising molecules is miR-21, which can serve as a biomarker with high potential for early diagnosis of various types of cancer. Here, we present a novel design of miR detection and demonstrate its efficacy on miR-21. The design employs emissive properties of DNA-silver nanoclusters (DNA/AgNC). The detection probe is designed as a hairpin DNA structure with one side of the stem complimentary to miR molecule. The binding of target miR-21 opens the hairpin structure, dramatically modulating emissive properties of AgNC hosted by the C12 loop of the hairpin. “Red” fluorescence of the DNA/AgNC probe is diminished in the presence of the target miR. At the same time, “green” fluorescence is activated and its intensity increases several-fold. The increase in intensity of “green” fluorescence is strong enough to detect the presence of miR-21. The intensity change follows the concentration dependence of the target miR present in a sample, which provides the basis of developing a new, simple probe for miR detection. The detection strategy is specific, as demonstrated using the response of the DNA/AgNC probe towards the scrambled miR-21 sequence and miR-25 molecule. Additionally, the design reported here is very sensitive with an estimated detection limit at ~1 picomole of miR-21.

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“…Gastric juice could be a good biomarker source for GC, as it is directly secreted from cells without elimination by the liver [ 105 ]. Altered expression levels of oncomiRs (miR-421, miR-21, and miR-106a) and tumor suppressor miRNAs (miR-129 and miR-133a) have been reported in GJ samples from patients with GC than those from without GC ( Figure 6 ).…”

Section: Analysis Of Gastric Juices/washesmentioning

confidence: 99%

Non-Invasive Early Molecular Detection of Gastric Cancers

Yamamoto

Watanabe

Sato

et al. 2020

Cancers

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Gastric cancer (GC) is a significant source of global cancer death with a high mortality rate, because the majority of patients with GC are diagnosed at a late stage, with limited therapeutic choices and poor outcomes. Therefore, development of minimally invasive or noninvasive biomarkers which are specific to GC is crucially needed. The latest advancements in the understanding of GC molecular landscapes and molecular biological methods have accelerated attempts to diagnose GC at an early stage. Body fluids, including peripheral blood, saliva, gastric juice/wash, urine, and others, can be a source of biomarkers, offering new methods for the early detection of GC. Liquid biopsy-based methods using circulating sources of cancer nucleic acids could also be considered as alternative strategies. Moreover, investigating gastric juices/washes could represent an alternative for the detection of GC via invasive biopsy. This review summarizes recently reported biomarkers based on DNA methylation, microRNA, long noncoding RNA, circular RNA, or extracellular vesicles (exosomes) for the detection of GC. Although the majority of studies have been conducted to detect these alterations in advanced-stage GC and only a few in population studies or early-stage GC, some biomarkers are potentially valuable for the development of novel approaches for an early noninvasive detection of GC.

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Gastric Juice MicroRNAs as Potential Biomarkers for Screening Gastric Cancer: A Systematic Review

Cited by 20 publications

References 12 publications

Biomarkers of gastric cancer: Current topics and future perspective

Biomarkers of gastric cancer: Current topics and future perspective

Micro RNA Sensing with Green Emitting Silver Nanoclusters

Non-Invasive Early Molecular Detection of Gastric Cancers

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