Gastrin-releasing peptide in human nasal mucosa.

Abstract

Gastrin-releasing peptide (GRP), the 27 amino acid mammalian form of bombesin, was studied in human inferior turbinate nasal mucosa. The GRP content of the mucosa measured by radioimmunoassay was 0.60±0.25 pmol/g tissue (n = 9 patients; mean±SEM). GRP-immunoreactive nerves detected by the immunogold method of indirect immunohistochemistry were found predominantly in small muscular arteries, arterioles, venous sinusoids, and between submucosal gland acini.I25I-GRP binding sites determined by autoradiography were exclusively and specifically localized to nasal epithelium and submucosal glands. There was no binding to vessels. The effects of GRP on submucosal gland product release were studied in short-term explant culture. GRP (10 ALM) significantly stimulated the release of the serous cell-specific product lactoferrin, and [3Hlglucosamine-labeled glycoconjugates which are products of epithelial goblet cells and submucosal gland cells. These observations indicate that GRP released from nerve fibers probably acts on glandular GRP receptors to induce glycoconjugate release from submucosal glands and epithelium and lactoferrin release from serous cells, but that GRP would probably not affect vascular permeability. (J. Clin. Invest. 1990. 85:998-1005.) gastrin-releasing peptide -human inferior turbinate nasal mucosa * respiratory glycoconjugateslactoferrin * explant culture

Gastrin releasing peptide (GRP) binding sites in human bronchi

“…This finding is consistent with the known functions of GRP. GRP and bombesin are potent stimuli of serous cell, mucous cell and possibly goblet cell secretion from human, cat and guinea pig upper and lower respiratory mucosa in vitro and in vivo (11)(12)(13)(14). Bombesin nasal provocation in humans induces both serous and mucous cell secretion without increasing vascular permeability (11).…”

“…Cryostat sections (lO).~m thick) were thaw mounted onto gelatinized slides, and warmed to room temperature. The slides were washed in CMRL media with aprotinin (400 kallikrein inhibitory units per ml, Sigma Chemical Co., St. Louis, MO, USA) and 0.5% bovine serum albumin for 30min at 25 "C (13,14). Slides were incubated for 75 min at 4°C with 1 nM 3-['251]-tyr'5-GRP (Amersham, Arlington Heights, IL, USA) in CMRL/aprotinin/ BSA.…”

“…Bombesin stimulate glandular secretion from both human and guinea pig nasal mucosa iin vivo (11,12). In vitro, GRP stimulates mucus selcretion from cat trachea (13) and human nasal mucosa (14). Both lactoferrin, a product of subtiucosal gland serous cells (15), and 3H-glucosaminelabelled respiratory glycoconjugates were released from human nasal mucosa (14).…”

“…In vitro, GRP stimulates mucus selcretion from cat trachea (13) and human nasal mucosa (14). Both lactoferrin, a product of subtiucosal gland serous cells (15), and 3H-glucosaminelabelled respiratory glycoconjugates were released from human nasal mucosa (14). GRP is1 also a stimulant of pancreatic exocrine secretion (16) and a participant in the central and vagal control of gastric mucosal homeostasis (17).…”

Imunofluorescência para neuropeptídeos na mucosa nasal humana: avaliação de técnica para peptídeo intestinal vasoativo (VIP)

“…Its presence in nervous fibers around the glands especially refers to the physiology of secretion. In the culture of human nasal mucosa cells, the increase of serous cell secretion after administration of VIP was observed, in addition to evidence of maximization of effect of cholinergic stimulation 18 , but there are studies that demonstrated secretion inhibitory action 10 . We observed increase in concentration of VIP (in addition to SP and CGRP) in nasal secretion of allergic subjects sensitized after nasal provocation.…”