Gastrinoma in vitro: Morphological and physiological studies of primary cell cultures

Gower, William R.; Ellison, E. Christopher; Knierim, Timothy H.; Elkhammas, Elmahdi A; O'Dorisio, Thomas M.; Fabri, Peter J.

doi:10.1016/0016-5085(90)90018-v

Cited by 10 publications

(2 citation statements)

References 42 publications

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“…Extracts of antrum were prepared by boiling washed and preweighed tissues for 10 min in 10 vol of 1 N acetic acid containing 0.1 mM phenylmethylsulfonyl fluoride, as described in detail previously (12). Gastrin (13), ANP, and proANP-(1-30) (11) concentrations in the supernatants were determined by specific RIAs as previously described. Gastrin antiserum 3021178 recognizes all amidated carboxy terminal molecular species (13).…”

Section: Methodsmentioning

confidence: 99%

“…Gastrin (13), ANP, and proANP-(1-30) (11) concentrations in the supernatants were determined by specific RIAs as previously described. Gastrin antiserum 3021178 recognizes all amidated carboxy terminal molecular species (13). ANP antiserum (Peninsula Laboratories) recognizes the carboxy terminal amino acids 99-126 of the prohormone, i.e., ANP (15).…”

Section: Methodsmentioning

confidence: 99%

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Regulation of atrial natriuretic peptide gene expression in gastric antrum by fasting

Gower

Salhab²,

Foulis³

et al. 2000

American Journal of Physiology-Regulatory, Integrative and Comp

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Atrial natriuretic peptide (ANP) gene expression was localized in the rat gastric antrum using immunohistochemistry and in situ hybridization to mucosal cells in the lower portion of the antropyloric glands. Colocalization of immunoreactive ANP, long-acting natriuretic peptide, i.e., proANP-(1-30), and serotonin in these cells identified them to be enterochromaffin cells. Fasting for 72 h in 8-mo-old (adult) rats produced a significant (P < 0.05) decrease in the levels of ANP prohormone mRNA, immunoreactive proANP-(1-30) and ANP to approximately 33% of that of fed rats. Fasting in 1-mo-old rats had no effect on these parameters. Transcripts for natriuretic peptide receptor subtypes NPR-A, NPR-B, and NPR-C were found in both mucosa and muscle tissues of the antrum. ANP, brain natriuretic peptide (BNP), and C-type natriuretic peptide (CNP) stimulated the production of cGMP in antral mucosa in vitro with a potency of ANP > BNP >> CNP, suggesting that these receptors were functional. We conclude that fasting decreases ANP prohormone mRNA and its gene products, long-acting natriuretic peptide, and ANP in the antrum of adult rats.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Regulation of atrial natriuretic peptide gene expression in gastric antrum by fasting

Gower

Salhab²,

Foulis³

et al. 2000

American Journal of Physiology-Regulatory, Integrative and Comp

Self Cite

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Differential Effects of Sodium Butyrate and Hexamethylene Bisacetamide on Growth and Secretion of Cultured Human Endocrine Tumor Cells

Parekh

Ishizuka

Townsend³

et al. 1991

Arch Surg

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Advanced gastrointestinal endocrine tumors respond poorly to conventional chemotherapy. In this study we examined the effects of two agents that promote cellular differentiation, sodium butyrate and hexamethylene bisacetamide, on the in vitro growth and secretory responses of a human pancreatic carcinoid (BON) and human gastrinoma (PT-2 and PT-SM) cell lines that have been established in our laboratory. We found that both sodium butyrate and hexamethylene bisacetamide strongly inhibited growth of BON, PT-2, and PT-SM cells. With continuous exposure of BON cells to sodium butyrate (2 mmol/L), the doubling time was prolonged, from 60 hours in controls to 156 hours, and saturation density was reduced to 28% that of controls. Hexamethylene bisacetamide (4 mmol/L) reduced saturation density to 37% that of controls in BON cells and prolonged the doubling time, from 60 hours to 103 hours. Antiproliferative effects of similar magnitudes were observed in the gastrinoma cell lines. In contrast, differential effects were produced on amine biosynthesis in BON cells; sodium butyrate stimulated levels of 5-hydroxytryptamine in the cells, whereas hexamethylene bisacetamide caused a profound dose-dependent inhibition of amine biosynthesis. The significant antiproliferative activity of sodium butyrate and hexamethylene bisacetamide and the inhibitory effects of hexamethylene bisacetamide on amine biosynthesis warrant evaluation of these agents or analogues for treatment of metastatic carcinoid and gastrinoma.

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Zollinger–Ellison Syndrome

Jensen

2015

Yamada' S Textbook of Gastroenterology

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Gastrinoma in vitro: Morphological and physiological studies of primary cell cultures

Cited by 10 publications

References 42 publications

Regulation of atrial natriuretic peptide gene expression in gastric antrum by fasting

Regulation of atrial natriuretic peptide gene expression in gastric antrum by fasting

Differential Effects of Sodium Butyrate and Hexamethylene Bisacetamide on Growth and Secretion of Cultured Human Endocrine Tumor Cells

Zollinger–Ellison Syndrome

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