Gastrodin alleviates rat chondrocyte senescence and mitochondrial dysfunction through Sirt3

Zhang, Yubiao; Jin, Wenyi; Chen, Junwen; Wei, Sixing; Cai, Wenxiang; Zhong, Yujian; Zhou, Yan; Peng, Hao

doi:10.1016/j.intimp.2023.110022

Cited by 13 publications

(5 citation statements)

References 32 publications

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“…Subsequently, a 60‐min blocking step was carried out with 5% bovine serum albumin (BSA). 30 Following this, the sections were incubated overnight at 4°C with primary antibodies. The primary antibodies employed encompassed anti‐Vimentin (diluted 1:200) and anti‐E‐cadherin (diluted 1:200) antibodies.…”

Section: Methodsmentioning

confidence: 99%

Integrating single‐cell and multi‐omic approaches reveals Euphorbiae Humifusae Herba‐dependent mitochondrial dysfunction in non‐small‐cell lung cancer

Zhang,

Zhao,

et al. 2024

J Cellular Molecular Medi

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Euphorbiae Humifusae Herba (EHH) is a pivotal therapeutic agent with diverse pharmacological effects. However, a substantial gap exists in understanding its pharmacological properties and anti‐tumour mechanisms. This study aimed to address this gap by exploring EHH's pharmacological properties, identifying NSCLC therapy‐associated protein targets, and elucidating how EHH induces mitochondrial disruption in NSCLC cells, offering insights into novel NSCLC treatment strategies. String database was utilized to explore protein–protein interactions. Subsequently, single‐cell analysis and multi‐omics further unveiled the impact of EHH‐targeted genes on the immune microenvironment of NSCLC, as well as their influence on immunotherapeutic responses. Finally, both in vivo and in vitro experiments elucidated the anti‐tumour mechanisms of EHH, specifically through the assessment of mitochondrial ROS levels and alterations in mitochondrial membrane potential. EHH exerts its influence through engagement with a cluster of 10 genes, including the apoptotic gene CASP3. This regulatory impact on the immune milieu within NSCLC holds promise as an indicator for predicting responses to immunotherapy. Besides, EHH demonstrated the capability to induce mitochondrial ROS generation and perturbations in mitochondrial membrane potential in NSCLC cells, ultimately leading to mitochondrial dysfunction and consequent apoptosis of tumour cells. EHH induces mitochondrial disruption in NSCLC cells, leading to cell apoptosis to inhibit the progress of NSCLC.

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Section: Methodsmentioning

confidence: 99%

Integrating single‐cell and multi‐omic approaches reveals Euphorbiae Humifusae Herba‐dependent mitochondrial dysfunction in non‐small‐cell lung cancer

Zhang,

Zhao,

et al. 2024

J Cellular Molecular Medi

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“…The cells were cultured in F12K or RPMI-1640, supplemented with 10% fetal bovine serum (FBS), 1% streptomycin, and penicillin. Maintained under conditions of 37°C, 5% CO2, and 95% humidity, the cell cultures were established ( 26 , 36 , 37 ). Total RNA from LUAD cells or tissues was isolated using the TRIzol reagent by Thermo Fisher Scientific, based in Waltham, MA, USA.…”

Section: Methodsmentioning

confidence: 99%

Revealing the role of regulatory T cells in the tumor microenvironment of lung adenocarcinoma: a novel prognostic and immunotherapeutic signature

Zhang,

Cui

et al. 2023

Front. Immunol.

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BackgroundRegulatory T cells (Tregs), are a key class of cell types in the immune system. In the tumor microenvironment (TME), the presence of Tregs has important implications for immune response and tumor development. Relatively little is known about the role of Tregs in lung adenocarcinoma (LUAD).MethodsTregs were identified using but single-cell RNA sequencing (scRNA-seq) analysis and interactions between Tregs and other cells in the TME were investigated. Next, we used multiple bulk RNA-seq datasets to construct risk models based on marker genes of Tregs and explored differences in prognosis, mutational landscape, immune cell infiltration and immunotherapy between high- and low-risk groups, and finally, qRT-PCR and cell function experiments were performed to validate the model genes.ResultsThe cellchat analysis showed that MIF-(CD74+CXCR4) pairs play a key role in the interaction of Tregs with other cell subpopulations, and the Tregs-associated signatures (TRAS) could well classify multiple LUAD cohorts into high- and low-risk groups. Immunotherapy may offer greater potential benefits to the low-risk group, as indicated by their superior survival, increased infiltration of immune cells, and heightened expression of immune checkpoints. Finally, the experiment verified that the model genes LTB and PTTG1 were relatively highly expressed in cancer tissues, while PTPRC was relatively highly expressed in paracancerous tissues. Colony Formation assay confirmed that knockdown of PTTG1 reduced the proliferation ability of LUAD cellsConclusionTRAS were constructed using scRNA-seq and bulk RNA-seq to distinguish patient risk subgroups, which may provide assistance in the clinical management of LUAD patients.

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“…Employ DAB staining, monitor the progress under an optical microscope, and then counterstain using hematoxylin. After each step, rinse with PBS ( 63 ).…”

Section: Methodsmentioning

confidence: 99%

Unveiling the role of regulatory T cells in the tumor microenvironment of pancreatic cancer through single-cell transcriptomics and in vitro experiments

Xu,

Zhang,

Zhao

et al. 2023

Front. Immunol.

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BackgroundIn order to investigate the impact of Treg cell infiltration on the immune response against pancreatic cancer within the tumor microenvironment (TME), and identify crucial mRNA markers associated with Treg cells in pancreatic cancer, our study aims to delve into the role of Treg cells in the anti-tumor immune response of pancreatic cancer.MethodsThe ordinary transcriptome data for this study was sourced from the GEO and TCGA databases. It was analyzed using single-cell sequencing analysis and machine learning. To assess the infiltration level of Treg cells in pancreatic cancer tissues, we employed the CIBERSORT method. The identification of genes most closely associated with Treg cells was accomplished through the implementation of weighted gene co-expression network analysis (WGCNA). Our analysis of single-cell sequencing data involved various quality control methods, followed by annotation and advanced analyses such as cell trajectory analysis and cell communication analysis to elucidate the role of Treg cells within the pancreatic cancer microenvironment. Additionally, we categorized the Treg cells into two subsets: Treg1 associated with favorable prognosis, and Treg2 associated with poor prognosis, based on the enrichment scores of the key genes. Employing the hdWGCNA method, we analyzed these two subsets to identify the critical signaling pathways governing their mutual transformation. Finally, we conducted PCR and immunofluorescence staining in vitro to validate the identified key genes.ResultsBased on the results of immune infiltration analysis, we observed significant infiltration of Treg cells in the pancreatic cancer microenvironment. Subsequently, utilizing the WGCNA and machine learning algorithms, we ultimately identified four Treg cell-related genes (TRGs), among which four genes exhibited significant correlations with the occurrence and progression of pancreatic cancer. Among them, CASP4, TOB1, and CLEC2B were associated with poorer prognosis in pancreatic cancer patients, while FYN showed a correlation with better prognosis. Notably, significant differences were found in the HIF-1 signaling pathway between Treg1 and Treg2 cells identified by the four genes. These conclusions were further validated through in vitro experiments.ConclusionTreg cells played a crucial role in the pancreatic cancer microenvironment, and their presence held a dual significance. Recognizing this characteristic was vital for understanding the limitations of Treg cell-targeted therapies. CASP4, FYN, TOB1, and CLEC2B exhibited close associations with infiltrating Treg cells in pancreatic cancer, suggesting their involvement in Treg cell functions. Further investigation was warranted to uncover the mechanisms underlying these associations. Notably, the HIF-1 signaling pathway emerged as a significant pathway contributing to the duality of Treg cells. Targeting this pathway could potentially revolutionize the existing treatment approaches for pancreatic cancer.

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Gastrodin alleviates rat chondrocyte senescence and mitochondrial dysfunction through Sirt3

Cited by 13 publications

References 32 publications

Integrating single‐cell and multi‐omic approaches reveals Euphorbiae Humifusae Herba‐dependent mitochondrial dysfunction in non‐small‐cell lung cancer

Integrating single‐cell and multi‐omic approaches reveals Euphorbiae Humifusae Herba‐dependent mitochondrial dysfunction in non‐small‐cell lung cancer

Revealing the role of regulatory T cells in the tumor microenvironment of lung adenocarcinoma: a novel prognostic and immunotherapeutic signature

Unveiling the role of regulatory T cells in the tumor microenvironment of pancreatic cancer through single-cell transcriptomics and in vitro experiments

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