Gastrointestinal nematode challenge induces some conserved gene expression changes in the gut mucosa of genetically resistant sheep

Ingham, Aaron; Reverter, Antônio; Windon, R.G.; Hunt, Peter W.; Menzies, Moira

doi:10.1016/j.ijpara.2007.07.012

Cited by 96 publications

(106 citation statements)

References 48 publications

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“…This finding supports earlier findings that resistance may be related to the host's ability to elicit a localized and rapid inflammatory response Ingham et al, 2008). Alternatively, by day 28 PI, the major pathways affected were related to upregulation of lipid metabolism and eicosanoid signaling genes, which the authors suggested may be related to a potential role of polyunsaturated fatty acids in the modulation of the immune system of cattle.…”

Section: Connor LI Baldwin and Lisupporting

confidence: 86%

“…Results indicated differential expression in both tissues in several functional classes of genes between the resistant and susceptible animals including transcripts encoding immunoglobulin (Ig) chains, integrins such as ITGA4, antigen presentation molecules such as CD45 and CD3E, tissue factors including DMBT1, PGA@, FGFR2 and TUBA2 and proinflammatory cytokine modulators of tumor necrosis factor and interferon. Of interest, mRNA for both IgE receptor and IFI35 were found to be differentially expressed between resistant and susceptible cattle, which had also been detected in divergently selected lines of parasite resistant sheep (Keane et al, 2006;Ingham et al, 2008). In general, the patterns of differential expression indicated a complex response to GI nematodes and no apparent single pathway related to resistance.…”

Section: Connor LI Baldwin and Limentioning

confidence: 95%

“…Using a similar approach, Ingham et al (2008) used realtime qRT-PCR to evaluate expression of 80 candidate genes in GI mucosa of two separate lines of sheep selected for resistance to GI nematodes. The two lines consisted of sheep selected for fecal egg counts after Haemonchus contortus challenge or immune response to vaccination with killed Trichostrongylus columbriformis larvae.…”

Section: Connor LI Baldwin and Limentioning

confidence: 99%

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Gene expression in the digestive tissues of ruminants and their relationships with feeding and digestive processes

Connor

Baldwin

et al. 2010

Animal

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The gastrointestinal tract (GIT) has multiple functions including digestion, nutrient absorption, secretion of hormones and excretion of wastes. In the ruminant animal, development of this organ system is more complex than that of the monogastric animal due to the necessity to establish a fully functional and differentiated rumen, in which a diverse microbial population of bacteria, fungi and protozoa support fermentation and digestion of dietary fiber. Central to the goal of animal scientists to enhance nutrient uptake and production efficiency of ruminants is the need for a comprehensive understanding of GIT development, as well as conditions that alter the digestion process. The relatively recent availability of genome sequence information has permitted physiological investigations related to the process of digestion for many agriculturally important species at the gene transcript level. For instance, numerous studies have evaluated the expression of ruminant GIT genes to gain insight into mechanisms involved in normal function, physiology and development, such as nutrient uptake and transport across the epithelial cell barrier throughout the alimentary canal, maintenance of rumen pH, and regulation of GIT motility and cell proliferation. Further, multiple studies have examined the effects of dietary modification, including feeding of supplemental fat, starch and protein, or a forage-v. concentrate-based diet on expression of critical gene pathways in the gut. In addition, the expression of genes in the GIT in response to disease, such as infection with gastrointestinal parasites, has been investigated. This review will summarize some of the recent scientific literature related to the gene expression in the GIT of ruminants, primarily cattle, sheep and goats, as it pertains to normal physiology, and dietary, developmental, and disease effects to provide an overview of critical proteins participating in the overall digestive processes, and their physiological functions. Recent findings from our laboratory will be highlighted also related to expression of the glucagon-like peptide two-hormone pathway in the GIT of dairy cattle during in various stages of the development and lactation, alterations in gene pathways associated with the rumen development and differentiation in the weaning calf, and genes of the GIT responding to Ostertagia, a common nematode infection of the cattle. Finally, prospective areas of investigation will be highlighted.

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Section: Connor LI Baldwin and Lisupporting

confidence: 86%

Section: Connor LI Baldwin and Limentioning

confidence: 95%

Section: Connor LI Baldwin and Limentioning

confidence: 99%

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Gene expression in the digestive tissues of ruminants and their relationships with feeding and digestive processes

Connor

Baldwin

et al. 2010

Animal

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“…This method accounts for "within gene across treatment" and "within treatment across gene" variation and has been used in the past (48,24,36). A general linear model approach was used consisting of the following: Expression (CT) ϳ state ϩ HGP ϩ Animal (state*HGP).…”

Section: Methodsmentioning

confidence: 99%

Chronic exposure to anabolic steroids induces the muscle expression of oxytocin and a more than fiftyfold increase in circulating oxytocin in cattle

Jager

Hudson

Reverter

et al. 2011

Physiological Genomics

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Chronic exposure to anabolic steroids induces the muscle expression of oxytocin and a more than fiftyfold increase in circulating oxytocin in cattle. Physiol Genomics 43: 467-478, 2011. First published February 15, 2011 doi:10.1152/physiolgenomics.00226.2010.-Molecular mechanisms in skeletal muscle associated with anabolic steroid treatment of cattle are unclear and we aimed to characterize transcriptional changes. Cattle were chronically exposed (68 Ϯ 20 days) to a steroid hormone implant containing 200 mg trenbolone acetate and 20 mg estradiol (Revalor-H). Biopsy samples from 48 cattle (half treated) from longissimus dorsi (LD) muscle under local anesthesia were collected. Gene expression levels were profiled by microarray, covering 16,944 unique bovine genes: 121 genes were differentially expressed (DE) due to the implant (99.99% posterior probability of not being false positives). Among DE genes, a decrease in expression of a number of fat metabolism-associated genes, likely reflecting the lipid storage activity of intramuscular adipocytes, was observed. The expression of IGF1 and genes related to the extracellular matrix, slow twitch fibers, and cell cycle (including SOX8, a satellite cell marker) was increased in the treated muscle. Unexpectedly, a very large 21-(microarray) to 97 (real time quantitative PCR)-fold higher expression of the mRNA encoding the neuropeptide hormone oxytocin was observed in treated muscle. We also observed an ϳ50-fold higher level of circulating oxytocin in the plasma of treated animals at the time of biopsy. Using a coexpression network strategy OXTR was identified as more likely than IGF1R to be a major mediator of the muscle response to Revalor-H. A re-investigation of in vivo cattle LD muscle samples during early to mid-fetal development identified a Ͼ128-fold increased expression of OXT, coincident with myofiber differentiation and fusion. We propose that oxytocin may be involved in mediating the anabolic effects of Revalor-H treatment.

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“…The alternative of repeated dosing with low numbers of L3 (continuous infection, similar to field challenge) would have required regular mustering of weaners for repeated dosing, with consequent detrimental effects on performance. In addition, the protocol conformed with infection regimes used for concurrent genomic studies in experimental resource flocks (Ingham et al 2008) and did not compromise selection for resistance, as demonstrated in previous research (Windon and Dineen 1981).…”

Section: Discussionmentioning

confidence: 94%

Design and phenotyping procedures for recording wool, skin, parasite resistance, growth, carcass yield and quality traits of the SheepGENOMICS mapping flock

et al. 2012

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Abstract.A major aim of the research program known as SheepGENOMICS was to deliver DNA markers for commercial breeding programs. To that end, a resource flock was established, comprehensively phenotyped and genotyped with DNA markers. The flock of nearly 5000 sheep, born over two consecutive years, was extensively phenotyped, with more than 100 recorded observations being made on most of the animals. This generated more than 460 000 records over 17 months of gathering information on each animal. Here, we describe the experimental design and sample-collection procedures, and provide a summary of the basic measurements taken. Data from this project are being used to identify collections of genome markers for estimating genomic breeding values for new sheep industry traits.

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Gastrointestinal nematode challenge induces some conserved gene expression changes in the gut mucosa of genetically resistant sheep

Cited by 96 publications

References 48 publications

Gene expression in the digestive tissues of ruminants and their relationships with feeding and digestive processes

Gene expression in the digestive tissues of ruminants and their relationships with feeding and digestive processes

Chronic exposure to anabolic steroids induces the muscle expression of oxytocin and a more than fiftyfold increase in circulating oxytocin in cattle

Design and phenotyping procedures for recording wool, skin, parasite resistance, growth, carcass yield and quality traits of the SheepGENOMICS mapping flock

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