Gastrointestinal nematodes infections and anthelmintic resistance in grazing sheep in the Eastern Inner Mongolia in China

Han, Ting; Wang, Min; Zhang, Guanghe; Han, Dongsheng; Wang, Zhe

doi:10.1515/ap-2017-0098

Cited by 16 publications

(12 citation statements)

References 18 publications

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“…The average infection rate of sheep GIN in eastern Inner Mongolia was reported at 79.2% with the highest infection rate being 100% that is consistent with the results of this study. However, the average infection intensity was 1813.2 that is higher than what we report [38]. The infection rate and infection intensity of this study were both higher than studies conducted…”

Section: Discussioncontrasting

confidence: 86%

An epidemiological study of gastrointestinal nematode and Eimeria coccidia infections in different populations of Kazakh sheep

Yan

Liu

et al. 2021

PLoS ONE

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This is an epidemiological study on the gastrointestinal nematode (GIN) and Eimeria coccidia infections in Kazakh sheep and the F1 and F2 generations of Kazakh × Texel sheep crosses. A total of 7599 sheep fecal samples were collected from the Zhaosu County and Nilka County in Ili Kazakh Autonomous Prefecture in the four seasons-spring, summer, autumn, and winter of 2019. The parasite causing the infection was identified by the saturated saline floating method, and the infection intensity was calculated by the modified McMaster method. SPSS19.0 was used to evaluate the differences in the fecal egg count (FEC) of for GIN and the fecal oocyst count (FOC) value of for coccidia per sample. The results showed that there were nine types of sheep GIN infections and Eimeria coccidia in these two counties of Ililocations, with the dominant parasite species of Haemonchus contortus, Trichostrongylus spp., and Ostertagia spp as the predominant parasites in the sheep. Most of the GIN and coccidia infections in these two regions were mild and moderate. The mean log (FEC) of GIN infection in the Zhaosu area was significantly higher than that in the Nilka area, whereas the mean log (FOC) of coccidia infection in Zhaosu was significantly lower than that of Nilka. The mean log (FEC) of GIN infection in the four seasons was the highest in spring, followed by in summer, then in autumn, and the lowest in winter. The mean log (FOC) of coccidia infection was the highest in spring, followed by in autumn, and was the lowest in summer and winter. The mean log (FEC) of GIN infection and log (FOC) of coccidia infection of Kazakh sheep was significantly higher than the F1 generation, which was then significantly higher than the F2 generation of summer. A positive correlation was found between the EPG and OPG levels in the sheep. These results showed that the GIN and coccidia infection intensities of the F1 generation sheep of Kazakh ×Texel crosses were significantly lower than that of Kazakh sheep paving the way for marker-based resistance selection.

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Section: Discussioncontrasting

confidence: 86%

An epidemiological study of gastrointestinal nematode and Eimeria coccidia infections in different populations of Kazakh sheep

Yan

Liu

et al. 2021

PLoS ONE

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“…Outside of QTP, there were some reports on livestock infection with GINs in other areas of China (Bhuiyan et al, 2017;T. Han, Wang, et al, 2017;Lv et al, 2016;Shen et al, 2019;Yuan et al, 2019), such as the sheep in inner Mongolian with 79.2% infection rate (T. Han, Wang, et al, 2017), which is consistent with the 78.8% ( 126 From a previous study, it was widely believed that T. axei, T. colubriformis, T. vitrinus and O. stephanostomum could infect humans (Bundy et al, 1985;Cibot et al, 2015;Elseadawy et al, 2021;Ghadirian & Arfaa, 1975;Phosuk et al, 2013) as zoonotic parasites, and there were already documents showing the infection with Trichostrongylus spp. in Henan (0.13%), Jiangsu (0.07%) and Jilin (0.06%) in local residents (Guan et al, 2015;Lee et al, 2017;Wei-Qi et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…GINs are the most common and economically destructive parasite in ruminants (El‐Alfy et al., 2019). Because of their diversity and different susceptibility to anthelmintic (T. Han, Wang, et al., 2017; Rashid et al., 2018), it is important to identify their species, hosts and infection sites to clarify which animals can be easily infected in specific areas.…”

Section: Discussionmentioning

confidence: 99%

Prevalence and molecular identification of gastrointestinal nematodes in Qinghai‐Tibetan Plateau of China

Ai,

Zhang,

Wang

et al. 2023

Veterinary Medicine & Sci

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BackgroundGastrointestinal nematodes (GINs) have seriously affected the production and earnings of animal husbandry in various countries, while some species of GINs infect humans. At present, little is known about the species and prevalence of GINs in Qinghai‐Tibetan Plateau (QTP).MethodsIn this study, 528 fresh faecal samples were collected from typical areas in different altitudes with seven species of livestock in Qinghai, Tibet, Gansu and Yunnan Provinces. ITS‐2 rRNA gene of nematodes was employed to detect by PCR and sequencing analysis. Phylogenetic analysis of related sequences was performed using MEGA 6.0 software.ResultsThe overall prevalence of GINs was 80.3% with 20 species of GINs detected, while Teladorsagia circumcincta was the dominant one, and four of which were zoonotic species such as Trichostrongylus colubriformis, Trichostrongylus axei, Trichostrongylus vitrinus and Oesophagostomum stephanostomum.ConclusionThe study provided panoptic information on the prevalence and species diversity of GINs in QTP area, which is useful and valuable for reference of measure formulation in livestock husbandry and public health concerns. The parasites of T. circumcincta, Cylicocyclus nassatus, Strongylus edentatus, Cylicostephanus longibursatus, Telephlebia brevicauda, Cyathostomum catinatum, Mecistocirrus digitatus, Cooperia punctata, Cylicodontophorus bicoronatus, Nematodirus oiratianus and Oesophagostomum asperum were firstly reported the presence in QTP area. The study also showed that horse could be infected by T. circumcincta, goat could be infected by C. nassatus, cattle could be infected by S. edentatus and C. bicoronatus,and O. stephanostomum could infect yak, cattle and Mongolian sheep in worldwide. Nevertheless, more investigations are needed, such as microscopic examination, to accurately determine the species in the region.

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“…Gastrointestinal parasitic infection is one of the major health problems in the world that causes huge economic loss in livestock production [38]. Several broad-spectrum anthelmintics are used to control these parasitic infections, but anthelminthic resistance of nematodes in small ruminants is becoming a serious threat in some countries [8][9][10][11]. Therefore, development of more effective control approaches is urgently needed.…”

Section: Discussionmentioning

confidence: 99%

“…Bloodfeeding adult H. contortus can lead to severe hemorrhagic gastritis, anemia, hypoproteinemia, edema, and even death in acute infections [5][6][7]. Control of this parasitic nematode is based on the application of anthelmintics, but excessive usage of these drugs has led to the development of anthelmintic resistance [8][9][10][11]. Previously, partial protection against H. contortus has been reported using DNA vaccines including actin [12], disorganized muscle family member-1 [13], cysteineprotease [14], glyceraldehyde-3-phosphate dehydrogenase [15] and glutathione peroxidase [16].…”

mentioning

confidence: 99%

Haemonchus contortus: siRNA mediated knockdown of matrix metalloproteinase 12A (MMP-12) results in reduction of infectivity

Naqvi

Gao

et al. 2020

Parasites Vectors

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Background: RNA interference (RNAi) is an important tool to determine the role of genes. RNAi has been widely used to downregulate target molecules, resulting in the reduction of mRNA for protein expression. Matrix metalloprotease 12A (MMP-12) is known to have important roles during embryonic development, organ morphogenesis and pathological processes in animals. However, MMP-12 from Haemonchus contortus has not been characterized.Methods: Haemonchus contortus MMP-12 gene was cloned and recombinant protein of MMP-12 (rHc-MMP-12) was expressed. Binding activities of rHc-MMP-12 to goat peripheral blood mononuclear cells (PBMCs) were assessed by immunofluorescence assay (IFA) and the immuno-regulatory effects of rHc-MMP-12 on cell proliferation and nitric oxide production were observed by co-incubation of rHc-MMP-12 with goat PBMCs. Furthermore, a soaking method was used to knockdown the expression of Hc-MMP12 gene using three siRNA, targeting different regions of the gene and infectivity of effective siRNA on the development of H. contortus was evaluated in goat.Results: rHc-MMP-12 was successfully expressed in an expression vector as well as the tissues of the cuticle of adult H. contortus worms and a successful binding with PBMCs surface were observed. Increased cellular proliferation and nitric oxide production by goat PBMCs was observed in a dose-dependent manner. Quantitative real time PCR (qRT-PCR) results confirmed the successful silencing of Hc-MMP-12 gene in siRNA of 1, 2 and 3 treated third-stage larvae (L3) of H. contortus in vitro. The most efficient qRT-PCR-identified siRNA template was siRNA-2, with a 69% suppression rate compared to the control groups. Moreover, in an in vivo study, silencing of the Hc-MMP-12 gene by siRNA-2 reduced the number of eggs (54.02%), hatchability (16.84%) and worm burden (51.47%) as compared to snRNAtreated control group. In addition, a shorter length of worms in siRNA-2-treated group was observed as compared to control groups. Conclusions:Our results indicate that siRNA-mediated silencing of Hc-MMP-12 gene in H. contortus significantly reduce the egg counts, larval hatchability, and adult worm counts and sizes. The findings of the present study demonstrate important roles of Hc-MMP-12 in the development of H. contortus.

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Gastrointestinal nematodes infections and anthelmintic resistance in grazing sheep in the Eastern Inner Mongolia in China

Cited by 16 publications

References 18 publications

An epidemiological study of gastrointestinal nematode and Eimeria coccidia infections in different populations of Kazakh sheep

An epidemiological study of gastrointestinal nematode and Eimeria coccidia infections in different populations of Kazakh sheep

Prevalence and molecular identification of gastrointestinal nematodes in Qinghai‐Tibetan Plateau of China

Haemonchus contortus: siRNA mediated knockdown of matrix metalloproteinase 12A (MMP-12) results in reduction of infectivity

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