Gastrointestinal pythiosis with concurrent presumptive gastrointestinal basidiobolomycosis in a Boxer dog

Parambeth, Joseph Cyrus; Lawhon, Sara D.; Mansell, Joanne; Wu, Jing; Clark, Sabrina D.; Sutton, Deanna A.; Gibas, Connie Fe C.; Wiederhold, Nathan P.; Myers, Alexandra; Johnson, Mark C.; Levine, Gwendolyn J.; Schlemmer, Samantha N.; Ruoff, Catherine M.; Isaiah, Anitha; Creevy, Kate E.

doi:10.1111/vcp.12720

Cited by 6 publications

(4 citation statements)

References 25 publications

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“…Histopathological findings from colonic tissues in both cases were characterized by eosinophilic granulomatous and pyogranulomatous inflammation associated with hyphae‐liked elements. Due to the histopathological similarities among fungal organisms, immunohistochemistry was required for differentiation (Parambeth et al., 2019 ). The definitive diagnosis of pythiosis in this study was made on the basis of immunohistochemistry and IPS.…”

Section: Discussionmentioning

confidence: 99%

Successful management of colonic pythiosis in two dogs in Thailand using antifungal therapy

Sukanan¹,

Suparp²,

Yongsiri³

et al. 2022

Veterinary Medicine & Sci

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Gastrointestinal pythiosis is a severe, progressive and often a fatal disease, which is caused by the aquatic pathogen Pythium insidiosum. Treatment is challenging due to the disease's resistance to antifungal drugs. Surgical resection is frequently attempted in cases of pythiosis; however, it can be technically challenging. This report presents two dogs with decreased appetite, abdominal pain, progressive haematochezia, tenesmus and significant weight loss. With the medical histories of both being young canines, living in areas with access to natural water resources and with the main chronic gastrointestinal symptoms having not responded to symptomatic treatment, pythiosis was taken into consideration. Abdominal ultrasound revealed severe, diffuse thickening and loss of normal layering of the colonic wall. These findings led to a differential diagnosis between intestinal neoplasia and fungal disease. Full‐thickness biopsies were later performed, and immunohistochemistry staining was suggested for colonic pythiosis. Medical treatment for pythiosis was successful with a combination of oral terbinafine and prednisolone. However, therapy with itraconazole in case 1 did not improve the clinical signs, and in case 2, itraconazole was used after all clinical signs have improved for clinical control. Since then, there has been no recurrence of clinical signs until the time of preparing this report (19 months for case 1, 11 months for case 2 since the cessation of treatment). The treatment was successful based on clinical signs and ultrasonographic data, and the disease remission was not confirmed by advance imaging, monitoring of pythiosis enzyme‐linked immunosorbent essay concentration or repeat sampling.

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Section: Discussionmentioning

confidence: 99%

Successful management of colonic pythiosis in two dogs in Thailand using antifungal therapy

Sukanan¹,

Suparp²,

Yongsiri³

et al. 2022

Veterinary Medicine & Sci

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“…P. insidiosum inhabits freshwater, where it colonizes a water plant and produces zoospores to complete its life cycle [3][4][5][6]. Clinical manifestations of pythiosis include cutaneous granulomatous ulcers [7,8], gastrointestinal lesion [9,10], corneal ulcers [11,12], arteritis [13,14], and disseminated infection [15,16]. A definitive diagnosis of pythiosis relies on culture-based identification [17][18][19][20], histopathological examination [21,22], serological assays [23][24][25][26][27], molecular methods [28][29][30][31][32][33], and proteomic assessment [34,35].…”

Section: Introductionmentioning

confidence: 99%

Selection of an Appropriate In Vitro Susceptibility Test for Assessing Anti-Pythium insidiosum Activity of Potassium Iodide, Triamcinolone Acetonide, Dimethyl Sulfoxide, and Ethanol

Yolanda

Lohnoo

Rujirawat

et al. 2022

JoF

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The orphan but highly virulent pathogen Pythium insidiosum causes pythiosis in humans and animals. Surgery is a primary treatment aiming to cure but trading off losing affected organs. Antimicrobial drugs show limited efficacy in treating pythiosis. Alternative drugs effective against the pathogen are needed. In-house drug susceptibility tests (i.e., broth dilution, disc diffusion, and radial growth assays) have been established, some of which adapted the standard protocols (i.e., CLSI M38-A2 and CLSI M51) designed for fungi. Hyphal plug, hyphal suspension, and zoospore are inocula commonly used in the drug susceptibility assessment for P. insidiosum. A side-by-side comparison demonstrated that each method had advantages and limitations. Minimum inhibitory and cidal concentrations of a drug varied depending on the selected method. Material availability, user experience, and organism and drug quantities determined which susceptibility assay should be used. We employed the hyphal plug and a combination of broth dilution and radial growth methods to screen and validate the anti-P. insidiosum activities of several previously reported chemicals, including potassium iodide, triamcinolone acetonide, dimethyl sulfoxide, and ethanol, in which data on their anti-P. insidiosum efficacy are limited. We tested each chemical against 29 genetically diverse isolates of P. insidiosum. These chemicals possessed direct antimicrobial effects on the growth of the pathogen in a dose- and time-dependent manner, suggesting their potential application in pythiosis treatment. Future attempts should focus on standardizing these drug susceptibility methods, such as determining susceptibility/resistant breakpoints, so healthcare workers can confidently interpret a result and select an effective drug against P. insidiosum.

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“…Direct exposure to the pathogen habitat (i.e., stagnant water, rice field, pond) could increase the risk of the infection [43][44][45][46][47]. Patients with pythiosis usually manifest with clinical features associated with an infection of the skin [11,23,48,49], artery [13,50,51], eye [47,[52][53][54], gastrointestinal tract [28,[55][56][57], or another internal organ [5,12,[58][59][60]. Diagnostic methods have been established for pythiosis [61], such as culture identification [62][63][64], histopathological examination [65][66][67][68], serological assays [69][70][71][72][73][74][75], molecular techniques [76][77][78][79][80][81][82], and proteomic analysis…”

Section: Introductionmentioning

confidence: 99%

History and Perspective of Immunotherapy for Pythiosis

Yolanda

Krajaejun

2021

Vaccines

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The fungus-like microorganism Pythium insidiosum causes pythiosis, a life-threatening infectious disease increasingly reported worldwide. Antimicrobial drugs are ineffective. Radical surgery is an essential treatment. Pythiosis can resume post-surgically. Immunotherapy using P. insidiosum antigens (PIA) has emerged as an alternative treatment. This review aims at providing up-to-date information of the immunotherapeutic PIA, with the focus on its history, preparation, clinical application, outcome, mechanism, and recent advances, in order to promote the proper use and future development of this treatment modality. P. insidiosum crude extract is the primary source of immunotherapeutic antigens. Based on 967 documented human and animal (mainly horses) pythiosis cases, PIA immunotherapy reduced disease morbidity and mortality. Concerning clinical outcomes, 19.4% of PIA-immunized human patients succumbed to vascular pythiosis instead of 41.0% in unimmunized cases. PIA immunotherapy may not provide an advantage in a local P. insidiosum infection of the eye. Both PIA-immunized and unimmunized horses with pythiosis showed a similar survival rate of ~70%; however, demands for surgical intervention were much lesser in the immunized cases (22.8% vs. 75.2%). The proposed PIA action involves switching the non-protective T-helper-2 to protective T-helper-1 mediated immunity. By exploring the available P. insidiosum genome data, synthetic peptides, recombinant proteins, and nucleic acids are potential sources of the immunotherapeutic antigens worth investigating. The PIA therapeutic property needs improvement for a better prognosis of pythiosis patients.

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Gastrointestinal pythiosis with concurrent presumptive gastrointestinal basidiobolomycosis in a Boxer dog

Cited by 6 publications

References 25 publications

Successful management of colonic pythiosis in two dogs in Thailand using antifungal therapy

Successful management of colonic pythiosis in two dogs in Thailand using antifungal therapy

Selection of an Appropriate In Vitro Susceptibility Test for Assessing Anti-Pythium insidiosum Activity of Potassium Iodide, Triamcinolone Acetonide, Dimethyl Sulfoxide, and Ethanol

History and Perspective of Immunotherapy for Pythiosis

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