Gastrointestinal Spindle Cell Tumours of the Dog: Histological and Immunohistochemical Study

Bettini, Giuliano; Morini, Maria; Marcato, P.S

doi:10.1016/s0021-9975(03)00046-x

Cited by 39 publications

(65 citation statements)

References 16 publications

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“…14,29,33,40,42 Canine GISTs share similar histological features, immunohistochemical expression, and activating c-KIT mutation (exon 11) with their human counterparts. 4,16,20 The canine DOG1 protein has high homology with the human protein (93% amino-acid sequence identity). In this study, we explored the expression of DOG1 in canine gastrointestinal sarcomas and normal intestine and found similar immunoreactivity to that reported in corresponding human tissues.…”

Section: Discussionmentioning

confidence: 99%

“…All tumors were primary mesenchymal tumors located within the gastrointestinal tract with histologic features consistent with GIST or leiomyosarcoma as previously described in both human and veterinary literature. 4,15,32,37 Tumors with benign characteristics consistent with leiomyoma, or with histologic characteristics of other primary tumors (adenocarcinoma, lymphoma), were not included in this study.…”

Section: Tumor Selectionmentioning

confidence: 99%

“…Consistent with other reports, none were located in the stomach. 4,18,22 The majority of tumors diagnosed as GIST (29, 87.9%) were made up of spindle-shaped cells. The remaining tumors (4, 12.1%) were of epithelioid (2; dogs 7 and 55) or mixed morphology (1; dog 49) with 1 tumor (dog 32) containing areas of moderately pleomorphic cells.…”

Section: Gastrointestinal Stromal Tumormentioning

confidence: 99%

“…Leiomyosarcomas more commonly stain positive for SMA and desmin. 4,16,18,22,43 In humans, ~5-10% of GISTs are KIT negative or only weakly positive. 8,14,33,36,52 These KIT-negative tumors are clinicopathologically indistinct from KIT-positive GISTs and have characteristic loss of chromosomes 1p, 14, and/or 578878V DIXXX10.1177/1040638715578878DOG1 immunohistochemical marker for diagnosis of canine GISTsDailey et al…”

Section: Introductionmentioning

confidence: 99%

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DOG1 is a sensitive and specific immunohistochemical marker for diagnosis of canine gastrointestinal stromal tumors

et al. 2015

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Abstract. Gastrointestinal stromal tumors (GISTs) and leiomyosarcomas are histologically similar primary neoplasms commonly occurring in the gastrointestinal tract of dogs and humans. Immunohistochemical staining (IHC) is needed to differentiate between these 2 entities and positive reactivity for KIT (cluster of differentiation [CD]117) is regarded as the gold standard for diagnosis of canine GIST. Studies estimate 5-10% of human GISTs stain negative or only weakly positive for KIT and have identified DOG1 (discovered on gastrointestinal stromal tumors protein 1) as a highly sensitive and specific marker for human GISTs. The purpose of this study was to evaluate immunoreactivity of a commercially available DOG1 antibody for use in diagnosis of canine GISTs. Fifty-five primary mesenchymal gastrointestinal tumors with histologic features consistent with GIST or leiomyosarcoma were evaluated via IHC for KIT, DOG1, and desmin. A subset of tumors was additionally evaluated for reactivity for smooth muscle actin (SMA). Thirty-three tumors (60%) were diagnosed as GIST based on positive immunoreactivity for KIT or DOG1 regardless of reactivity for desmin or SMA. Most GISTs (32/33, 97.0%) had similar staining for both KIT and DOG1. DOG1 expression was identified in 2 tumors (1 study tumor and 1 additional tumor) negative for KIT and desmin that had histologic features consistent with KIT-negative, platelet-derived growth factor receptor-alpha (PDGFRA)-mutant human GISTs. Our results suggest that DOG1 has improved specificity and sensitivity to that of KIT for differentiating between canine GISTs and leiomyosarcomas. Inclusion of both DOG1 and KIT IHC in diagnostic panels will improve the accuracy of canine GIST diagnosis.

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Section: Discussionmentioning

confidence: 99%

Section: Tumor Selectionmentioning

confidence: 99%

Section: Gastrointestinal Stromal Tumormentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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DOG1 is a sensitive and specific immunohistochemical marker for diagnosis of canine gastrointestinal stromal tumors

et al. 2015

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“…Based on both immunophenotyping and ultrastructure, in its "Classification of Tumors of Domestic Animals" the World Health Organization separates both benign and malignant smooth muscle tumors from GISTs, which are composed of a mixture of cells with neurogenic and myogenic characters (Head, 2003-WHO), which probably arise from the interstitial cells of Cajal (Miettinen, 2001). In addition to this, leiomyomas and leiomyosarcomas in dogs typically are desminand α-SMA-positive and CD117-negative (Bettini, 2003), while GIST prove desmin-negative. …”

Section: Resultsmentioning

confidence: 99%