Gastrointestinal stromal tumors (GISTs): CD117, DOG-1 and PKCθ expression. Is there any advantage in using several markers?

Rios-Moreno, María José; Jaramillo, Sara; Gallardo, S. Pereira; Vallejo, Ana Isabel; Mora, M. Molina; García-Escudero, Antonio; Amérigo, J; González‐Cámpora, Ricardo

doi:10.1016/j.prp.2011.11.006

Cited by 34 publications

(42 citation statements)

References 29 publications

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“…The rate of DOG1 positivity was 20-100% in CD117 negative GIST cases in different studies, all of which reported that DOG1 should be added to the immunohistochemical panel in the diagnostic steps independent of the mutation status (2,(6)(7)(8)29,31,38,42). In the present study, positivity with CD117 was provided by applying the immunohistochemical studies on multiple different paraffin blocks in those cases which could be negative for CD117 (focally and weak reaction).…”

Section: Table 5 Correlation Between Dog1 Immunoexpression and Risk mentioning

confidence: 69%

“…However, the DOG1 positivity rate varies between 37 and 100% in CD117 negative cases even in studies performed with the DOG1/K9 clone (2,7,8,38). The wide range of DOG1 positivity in CD117 negative cases in such series may be due to the low number of cases, differences among the DOG1 clones used or other unknown factors.…”

Section: Table 5 Correlation Between Dog1 Immunoexpression and Risk mentioning

confidence: 89%

“…32, No. 4,2015 been produced and used in various studies (2)(3)(4)(5)(6)(7)(8)29,31,38). As summarized in the article of Wong et al (15), the difference in positivity rates is thought to stem from the sensitivity features of the clone used.…”

Section: Table 5 Correlation Between Dog1 Immunoexpression and Risk mentioning

confidence: 99%

“…As summarized in Table 6, collective evaluation of the results of the series where CD117 and DOG1 antibodies were studied together (if we ignore the fact that these studies were performed under different laboratory conditions at different centers and by using different antibody clones) in GISTs (2)(3)(4)(6)(7)(8)(29)(30)(31)38) revealed that the CD117 positivity rate was 91% and DOG1 positivity 93% in about three thousand GIST cases. DOG1 specificity was reported to be a little lower than, equal to or greater than CD117 specificity in different studies.…”

Section: Table 5 Correlation Between Dog1 Immunoexpression and Risk mentioning

confidence: 99%

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Histopathological Features of Gastrointestinal Stromal Tumors and the Contribution of DOG1 Expression to the Diagnosis

Guler¹,

Özyilmaz²,

Tokuç³

et al. 2015

Balkan Med J

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Background:Gastrointestinal stromal tumors (GIST) have KIT or platelet-derived growth factor receptor α (PDGFRα) mutations affecting receptor tyrosine kinase activity and do not benefit from classic treatment regimens. Aims: The aim of this study was to review the algorithm that may be followed for the diagnosis and differential diagnosis in GISTs by investigating the histomorphological parameters and expression characteristics of classical immunohistochemical antibodies used in routine tests in addition to DOG1 expression. Study Design: Diagnostic accuracy study. Methods: We reevaluated the histological and immunohistochemical parameters of 37 GISTs. The standard immunohistochemical diagnosis and differential diagnosis panel antibodies (CD117, PDGFRα, CD34, vimentin, desmin, SMA, S-100, and Ki67) were studied on the tumor sections. We also used the popular marker DOG1 antibody with accepted sensitivity for GISTs in recent years and the PDGFRα immune marker for which the benefit in routine practice is discussed. Results: Classification according to progressive disease risk groups of the 37 cases revealed that 54% were in the high risk, 19% in the moderate risk, 16% in the low risk, 8% in the very low risk and 8% in the no risk group. Cytological atypia, necrosis, mucosal invasion and the Ki67 index were found to be related to the progressive disease risk groups of the tumors (p<0.05). Positive immunoreaction was observed with CD117 and PDGFRα in all GISTs in the study (100%). Positivity with the DOG1 antibody was found in 33 (89%) cases. CD34 was positive in 62% (23) of the cases. Conclusion: The CD117 antibody still plays a key role in GIST diagnosis. However, the use of DOG1 and PDGFRα antibodies combined with CD117 as sensitive markers can be beneficial.

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Section: Table 5 Correlation Between Dog1 Immunoexpression and Risk mentioning

confidence: 69%

Section: Table 5 Correlation Between Dog1 Immunoexpression and Risk mentioning

confidence: 89%

Section: Table 5 Correlation Between Dog1 Immunoexpression and Risk mentioning

confidence: 99%

Section: Table 5 Correlation Between Dog1 Immunoexpression and Risk mentioning

confidence: 99%

See 2 more Smart Citations

Histopathological Features of Gastrointestinal Stromal Tumors and the Contribution of DOG1 Expression to the Diagnosis

Guler¹,

Özyilmaz²,

Tokuç³

et al. 2015

Balkan Med J

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“…The diagnosis was verified by immunohistochemical staining for CD117 and DOG1, which are known to be markers for GISTs (12).…”

Section: Discussionmentioning

confidence: 95%

Primary extragastrointestinal stromal tumor of the pleura: A case report

Zhang

Liu

et al. 2016

Oncology Letters

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Abstract. Gastrointestinal stromal tumors (GISTs) are the most common type of mesenchymal tumor of the gastrointestinal tract. The stomach and small intestine are the most common sites of occurrence. GISTs are mesenchymal neoplasms originating from the interstitial cells of Cajal (ICCs), and are characterized by positivity for cluster of differentiation (CD) 117, also known as proto-oncogene c-Kit. While the majority of GISTs develop in the alimentary tract, in rare cases they may also be found in extragastrointestinal tissues. This type of GIST is known as an extragastrointestinal stromal tumor (EGIST). Despite the fact that EGISTs have been reported in the mesentery, omentum and retroperitoneum, primary intrathoracic EGISTs, arising from the pleura or lungs, are rare. The patient presented in the current study was a 40-year-old man, who presented with a cough and pyrexia, with pleural effusion on the left side. Multiple nodules throughout the parietal pleura were identified by thoracoscopy and a diagnosis of primary GIST of pleura was established, since they were positive for CD117 and discovered on GIST-1 and there was no evidence of gastrointestinal tumors. Subsequently, the patient was administered with imatinib and had no signs of disease recurrence 2 years later.

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Gastrointestinal stromal tumors (GISTs): SEAP–SEOM consensus on pathologic and molecular diagnosis

et al. 2016

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Gastrointestinal stromal tumors (GISTs) are the most common mesenchymal neoplasms of the digestive tract, with an incidence of 1.1 cases/100,000 inhabitants/year. A group of experts from the Spanish Society of Pathology and the Spanish Society of Oncology met to discuss a brief update on GISTs and agree on aspects relating to the pathological and molecular diagnosis of these tumors. GISTs are generally solitary, well-circumscribed lesions of variable size (<10 mm-35 cm) that may present with intra- or extra-luminal parietal growth or a mixed-type (hourglass) growth pattern. Histologically, they are unencapsulated neoplasms displaying expansive growth and spindle-shaped (70%), epithelioid (20%), or mixed cellularity (10%). Mitotic activity is generally moderate or low and should be evaluated only in areas with high cellularity or higher mitotic frequency. The great majority of GISTs harbour mutually exclusive activating mutations in genes coding for the type III receptor tyrosine kinases KIT and PDGFRA; less commonly, GISTs have also been reported to display mutations elsewhere, including BRAF and NF1 and SDH-complex genes. The method most widely used to detect KIT and PDGFRA mutations is amplification of the exons involved by polymerase chain reaction followed by direct sequencing (Sanger method) of these amplification products. Molecular analyses should always specify the type of analysis performed, the region or mutations evaluated, and the sensitivity of the detection method employed.

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Gastrointestinal stromal tumors (GISTs): CD117, DOG-1 and PKCθ expression. Is there any advantage in using several markers?

Cited by 34 publications

References 29 publications

Histopathological Features of Gastrointestinal Stromal Tumors and the Contribution of DOG1 Expression to the Diagnosis

Histopathological Features of Gastrointestinal Stromal Tumors and the Contribution of DOG1 Expression to the Diagnosis

Primary extragastrointestinal stromal tumor of the pleura: A case report

Gastrointestinal stromal tumors (GISTs): SEAP–SEOM consensus on pathologic and molecular diagnosis

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