Gastroprotective activity of Pradosia huberi on experimentally induced gastric lesions in rodents: Role of endogenous sulphydryls and nitric oxide

Kushima, Hélio; Hiruma-Lima, Clélia Akiko; Santos, Maria Aparecida dos; Viana, Elizabeth; Coelho–Ferreira, Márlia; Brito, Alba Regina Monteiro Souza

doi:10.1016/j.jep.2005.03.014

Cited by 24 publications

(21 citation statements)

References 17 publications

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“…Gastric lesions induced by NSAIDs are caused by the suppression of prostaglandin biosynthesis and gastric damage induced by ethanol causes impairment of gastric defensive factors such as mucus and mucosa circulation (Kushima et al, 2005). When we compared to the control value, Cc significantly inhibited ulcerative lesions in all doses, but the best result was obtained after pretreatment with Cc using the absolute ethanol model (Table 1).…”

Section: Resultsmentioning

confidence: 99%

Gastroprotective effect of Cissus sicyoides (Vitaceae): Involvement of microcirculation, endogenous sulfhydryls and nitric oxide

Ferreira¹,

Nishijima²,

Seito³

et al. 2008

Journal of Ethnopharmacology

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Section: Resultsmentioning

confidence: 99%

Gastroprotective effect of Cissus sicyoides (Vitaceae): Involvement of microcirculation, endogenous sulfhydryls and nitric oxide

Ferreira¹,

Nishijima²,

Seito³

et al. 2008

Journal of Ethnopharmacology

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“…The lesions were long, hemorrhic and confined to the glandular portions (Table 1). Alcoholinduced gastric lesions impaired gastric defensive factors such as mucus and mucosa circulation (Kushima et al, 2005). Treatment with S. lavandulifolia extract could partly reduce the ulcer index and promote gastric healing, but it could not significantly improve gastric lesions of Lvandu/alcohol group (Table 1).…”

Section: Discussionmentioning

confidence: 97%

Gastroprotective effects of Stachys Lavandulifolia extract on experimental gastric ulcer

Nabavizadeh¹

2011

Afr. J. Pharm. Pharmacol.

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Several studies have used Stachys Lavandulifolia vahl (S. lavandulifolia) as medicinal plant in Iranian folk medicine. The present investigation is designed to elucidate therapeutic and preventive effects of S. lavandulifolia extract on gastric acid and pepsin secretions in experimental gastric ulcer. Thirty two Wistar male rats were used to study therapeutic and preventive effects of S. lavandulifolia extract on alcohol-induced gastric ulcer. Animals were equally (n=8) divided into 4 groups: (I) Control (II) Alcohol (1 ml/200 g/bw) to induce gastric ulcer (III) Alcohol/Lvandu (100 mg/kg bw/daily, S. lavandulifolia extract was given for two weeks post alcohol administration) and (IV) Lvandu/alcohol (S. lavandulifolia extract was given for two weeks before alcohol administration). Ulcer index, gastric acid and pepsin secretions was measured. Ulcer index was significantly decreased in alcohol/Lvandu than Alcohol group. Also gastric acid and pepsin secretions, and gastric tissue's NO metabolites level were significantly lower in alcohol and Lvandu/alcohol groups than control (p<0/05). But changes in gastric acid and pepsin secretions have not been noticeable in alcohol/Lvandu group than control. The results of this study show that S. lavandulifolia extract protected gastric mucosa from alcohol-induced gastric ulcer. This gastroprotection may mediate via gastric mucosal nitric oxide production.

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“…There are few phytochemical and biological studies of P. huberi reported in the literature; however, the hydroalcoholic extract from P. huberi bark has shown antisecretory and gastroprotective activity, besides no acute toxicity (5000 mg/kg; p.o.) (Kushima et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Effects of extract, fractions and 2,3-dihydromyricetin-3-O-α-L-rhamnoside from Pradosia huberi (Ducke) Ducke on rat isolated mesenteric arteries

Medeiros

Queiroz³

et al. 2010

Rev. bras. farmacogn.

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RESUMO: "Efeito do extrato, frações e da 2,3-diidromiricetina-3-O-α-L-raminosídeo obtidos da Pradosia huberi (Ducke) Ducke em artéria mesentérica isolada de rato". Pradosia huberi (Ducke) Ducke (Sapotaceae), espécie Amazônica popularmente conhecida como "casca-doce" é utilizada na medicina tradicional no tratamento de gastrite. O extrato etanólico de suas cascas é rico em polifenóis que podem apresentar um grande número de atividades, incluindo efeito vasorelaxante e cardioprotetor. O objetivo deste estudo foi avaliar as propriedades farmacológicas do extrato etanólico (EPH), de frações e da 2,3-diidromiricetina-3-O-α-L-raminosídeo isolados de P. huberi, em artéria mesentérica isolada de rato. O EPH foi fracionado resultando nas seguintes frações: CHCl 3 , CHCl 3 :AcOEt (1:1), AcOEt, AcOEt:MeOH (1:1) e MeOH. Da fração MeOH foi isolada a 2,3-diidromiricetina-3-O-α-L-raminosídeo e identificada através de espectro de RMN de 1 H e 13 C, além de comparações com os dados de literatura. EPH (1-100 μg/mL) promoveu relaxamento dependente de concentração no tônus vascular induzido por 10 μM de fenilefrina (CE 50 =17,1±2,9 µg/ mL; E max =87,4±2,9 %, n=8). A fração MeOH também relaxou os anéis mesentéricos (CE 50 =31±2,0 µg/mL; E max =54±12,5%, n=6), porém com menor eficácia quando comparado ao efeito de EPH. Unitermos: Pradosia huberi, artéria mesentérica, vasodilatação, endotélio dependente, 2,3-diidromiricetina-3-O-α-L-raminosídeo, floresta amazônica.ABSTRACT: Pradosia huberi (Ducke) Ducke (Sapotaceae), an Amazonian species, is popularly known as "casca-doce" and used in the folk medicine for the treatment of gastritis. The ethanol extract of the bark contains mainly polyphenolic compounds, which are known to show a large number of activities, including cardioprotective and vasorelaxant effects. The aim of this study was to evaluate the pharmacological properties induced by P. huberi ethanol extract (PHEE) and fractions and 2-3-dihydromyricetin-3-O-α-L-rhamnoside derived from this extract, in isolated rat mesenteric arteries. PHEE was separated and the following fractions were obtained: CHCl 3 , CHCl 3 :AcOEt (1:1), AcOEt, AcOEt:MeOH (1:1) and MeOH. We isolated 2-3-dihydromyricetin-3-O-α-L-rhamnoside from the MeOH fraction, which was identified by 1 H and 13 C NMR spectra and compared with data in the literature. PHEE (1-100 μg/mL) induced concentration-dependent relaxations of 10 μM phenylephrine-induced tone (EC 50 =17,1±2,9 µg/mL; E max =87.4±2.9 %, n=8). The MeOH fraction also relaxed mesenteric rings (EC 50 =31±2.0 μg/mL; E max =54±12.5%, n=6) but less effectively when compared to PHEE. Both effects were completely abolished after removal of the vascular endothelium. The AcOEt:MeOH (1:1) fraction and the isolated flavonoid were ineffective in eliciting vasorelaxation. The study demonstrates that PHEE and MeOH fraction of Pradosia huberi possess a vasorelaxant effect, which may be completely dependent upon endothelium. The isolated flavonoid is not responsible for this vasorelaxant effect.

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Gastroprotective activity of Pradosia huberi on experimentally induced gastric lesions in rodents: Role of endogenous sulphydryls and nitric oxide

Cited by 24 publications

References 17 publications

Gastroprotective effect of Cissus sicyoides (Vitaceae): Involvement of microcirculation, endogenous sulfhydryls and nitric oxide

Gastroprotective effect of Cissus sicyoides (Vitaceae): Involvement of microcirculation, endogenous sulfhydryls and nitric oxide

Gastroprotective effects of Stachys Lavandulifolia extract on experimental gastric ulcer

Effects of extract, fractions and 2,3-dihydromyricetin-3-O-α-L-rhamnoside from Pradosia huberi (Ducke) Ducke on rat isolated mesenteric arteries

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