Gastroprotective effect and in vitro Antioxidant Activities of the Aqueous Extract from Artemisia absinthium L Aerial Parts

Kaoudoune, Chahrazed; Benchikh, Fatima; Benabdallah, Hassiba; Loucif, Karima; Mehlous, Soulaf; Amira, Smain

doi:10.22270/jddt.v10i4.4253

Cited by 8 publications

(4 citation statements)

References 19 publications

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“…Teas are known to have a high content of polyphenolics and it is known that total polyphenols have a strong correlation with antioxidant capacity. Previous studies of the antioxidant properties of aqueous extracts of the selected plants (S1–S4) reported TP content of 39.58 ± 6.01 mg GAE/g for A. annua [ 78 ], 58.66 ± 2.16 μg GAE/mg dw [ 79 ] and 134.47 mg GAE/100 g dw [ 80 ] for A. abisinhtium , 10.29 ± 0.04 mg GAE/g dw for O. vulgare [ 81 ] and 230.8 mg GAE/g dw for M. pipperita [ 82 ]. As it can be seen from Table 2 , higher TP content was recorded for plants harvested and processed in Herzegovina, which varied from 6.0343 mmoL GAE/g dw to 9.472 mmoL GAE/g dw.…”

Section: Resultsmentioning

confidence: 99%

Antioxidant Capacity of Herzegovinian Wildflowers Evaluated by UV–VIS and Cyclic Voltammetry Analysis

et al. 2022

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Considering the vast cultural and traditional heritage of the use of aromatic herbs and wildflowers for the treatment of light medical conditions in the Balkans, a comparison of the antioxidant capacity of wildflowers extracts from Herzegovina was studied using both cyclic voltammetry and spectrophotometry. The cyclic voltammograms taken in the potential range between 0 V and 800 mV and scan rate of 100 mV s−1 were used for the quantification of the electrochemical properties of polyphenols present in four aqueous plant extracts. Antioxidant capacity expressed as mmoL of gallic acid equivalents per gram of dried weight of the sample (mmoL GAE g−1 dw) was deduced from the area below the major anodic peaks (Q400 pH 6.0, Q500 pH 4.7, Q600 pH 3.6). The results of electrochemical measurements suggest that the major contributors of antioxidant properties of examined plants are polyphenolic compounds that contain ortho-dihydroxy-phenol or gallate groups. Using Ferric reducing-antioxidant power (FRAP) and 2,2′-azino-bis spectrophotometric methods (3-ethylbenzthiazoline-6-sulphonic acid) radical cation-scavenging activity (ABTS) additionally determined antioxidant capacity. The FRAP results ranged from 2.9702–9.9418 mmoL Fe/g dw, while the results for ABTS assays expressed as Trolox equivalents (TE) ranged from 14.1842–42.6217 mmoL TE/g dw. The Folin–Ciocalteu procedure was applied to determine the total phenolics content (TP). The TP content expressed as Gallic acid equivalents (GAE) ranged from 6.0343–9.472 mmoL GAE/g dw. The measurements of total flavonoid (TF) and total condensed tannin (TT) contents were also performed to obtain a broader polyphenolic profile of tested plant materials. Origanum vulgare L. scored the highest on each test, with the exception of TT content, followed by the Mentha × piperita L., Artemisia annua L., and Artemisia absinthium L., respectively. The highest TT content, expressed as mg of (−)catechin equivalents per gram of dried weight of sample (mg CE/g dw), was achieved with A. absinthium extract (119.230 mg CE/g dw) followed by O. vulgare (90.384 mg CE/g dw), A. annua (86.538 mg CE/g dw) and M. piperita (69.231 mg CE/g dw), respectively. In addition, a very good correlation between electrochemical and spectroscopic methods was achieved.

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Section: Resultsmentioning

confidence: 99%

Antioxidant Capacity of Herzegovinian Wildflowers Evaluated by UV–VIS and Cyclic Voltammetry Analysis

et al. 2022

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“…In other species belonging to the Artemisia genus, anti-inflammatory and gastric anti-ulcer effects have been evaluated in different models. For instance, gastroprotection has been reported for the aqueous extracts of A. absinthium (91% with 400 mg/kg) [43], A. capillaris (85% with 200 mg/kg) [44] and a pectic fraction of an aqueous extract from A. campestris (77% with 0.3 mg/kg) [45]. Their effect was attributed to their antioxidant action and anti-inflammatory capacity, and to the improvement of gastric mucosa protective mechanisms [43][44][45].…”

Section: Discussionmentioning

confidence: 99%

Anti-Helicobacter pylori Activity of Artemisia ludoviciana subsp. mexicana and Two of Its Bioactive Components, Estafiatin and Eupatilin

et al. 2021

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Artemisia ludoviciana subsp. mexicana, whose main etiological agent is Helicobacter pylori, has been traditionally used for the treatment of digestive ailments such as gastritis. In a previous screening study, the aqueous extract exhibited good in vitro anti-H. pylori activity. With the aim of determining the efficacy of this species as a treatment for H. pylori related diseases and finding bioactive compounds, its aqueous extract was subjected to solvent partitioning and the fractions obtained were tested for their in vitro anti-H. pylori effect, as well as for their in vivo gastroprotective and anti-inflammatory activities. The aqueous extract showed a MIC = 250 µg/mL. No acute toxicity was induced in mice. A gastroprotection of 69.8 ± 3.8%, as well as anti-inflammatory effects of 47.6 ± 12.4% and 38.8 ± 10.2% (by oral and topical administration, respectively), were attained. Estafiatin and eupatilin were isolated and exhibited anti-H. pylori activity with MBCs of 15.6 and 31.2 µg/mL, respectively. The finding that A. ludoviciana aqueous extract has significant anti-H. pylori, gastroprotective and anti-inflammatory activities is a relevant contribution to the ethnopharmacological knowledge of this species. This work is the first report about the in vivo gastroprotective activity of A. ludoviciana and the anti-H. pylori activity of eupatilin and estafiatin.

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“…One of the reasons is that this method is simple and highly sensitive. The DPPH radical is often used as an indicator to test the ability of the extract to give a hydrogen atom or an electron and therefore its anti-radical or antioxidant capacity 22 . The plant extract in study shows a powerful scavenging activity (IC = 0.050 ± 0.0018 mg/ml).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Antioxidant activities and total phenolic content of hydro-ethanol extract from Phlomis bovei De Noé areal parts

Amira

Amira²,

Benchikh³

et al. 2020

J. Drug Delivery Ther.

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The plants of the genus Phlomis are native to Turkey, North Africa, Europe and Asia. Phlomis bovei De Noé (Lamiaceae) is a rare Algerian endemic plant, commonly known as Kayat El Adjarah. The objective of this study is to quantify the polyphenol content and to evaluate the antioxidant activities of the ethanolic extract (EthE) of Phlomis bovei De Noé. The plant extract was prepared by macerating 100 g of ground material in 1000 ml of 85 % ethanol for 72 h, then the filtrate was evaporated using a rotary evaporator at a temperature of 45C and the filtrate was dried. The total content in polyphenols was determined using Folin Ciocalteu method. Flavonoid content in extracts was determined using Aluminum trichloride assay and the total content of tannins was determined according to Bate Smith method. The antioxidant activity was investigated in vitro by the DPPH (2, 2-diphenyl-1-picryl-hydrazyl), ABTS and iron chelating assays. The results indicate that the ethanol extract was rich in polyphenols and has an important capacity of scavenging the DPPH and ABTS free radical with an IC50 of 0.05 and 0.018 mg/ml, respectively. However, in the ferrous ions chelating ability test, PBEE extract showed moderate chelating activity (IC50 = 1.59 ± 0.05 mg/ml). To conclude, the obtained results may contribute to add possible scientific data to use Phlomis bovei industrially as herbal medicine and as supplementary sources for natural antioxidant drugs in food. Keywords: antioxidant activity, ethanol extract, Phlomis bovei De Noé, polyphenol content.

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Gastroprotective effect and in vitro Antioxidant Activities of the Aqueous Extract from Artemisia absinthium L Aerial Parts

Cited by 8 publications

References 19 publications

Antioxidant Capacity of Herzegovinian Wildflowers Evaluated by UV–VIS and Cyclic Voltammetry Analysis

Antioxidant Capacity of Herzegovinian Wildflowers Evaluated by UV–VIS and Cyclic Voltammetry Analysis

Anti-Helicobacter pylori Activity of Artemisia ludoviciana subsp. mexicana and Two of Its Bioactive Components, Estafiatin and Eupatilin

Evaluation of Antioxidant activities and total phenolic content of hydro-ethanol extract from Phlomis bovei De Noé areal parts

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