Gastroprotective effect of hydromethanolic extract of
            <i>Ayapana triplinervis</i>
            leaves on indomethacin‐induced gastric ulcer in male Wistar rats

Maity, Ratnabali; Mondal, Prabir; Giri, Mukul Kumar; Ghosh, Chaitali; Mallick, Chhanda

doi:10.1111/jfbc.13859

Cited by 13 publications

(5 citation statements)

References 52 publications

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“…Elevation of CD and MDA levels in gastric tissue in indomethacin treated rats indicates the involvement of oxidative injury 46,47 . Hydromethanol extract of leaves of AT have the potent antioxidant activity that scavenged the free radicals and protects the rats from indomethacin induced oxidative damage 20 .…”

Section: Discussionmentioning

confidence: 99%

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2024

IJPSR

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Peptic ulcer disease (PUD) is increasing gradually in India as well as worldwide. Excess use of non-steroidal anti-inflammatory agents (NSAID) and lifestyle factors cause gastric mucosal damage. This study was designed to search out the gastro-protective efficacy of hydromethanolic extract of Ayapana triplinervis on different graded ulcers. Mild to severe grade of gastric ulcer has been developed by oral administration of IND at the doses of 10mg, 20mg and 40mg/kg body weight for 15 days. Gastric ulcer were assessed by elevation of ulcer score, ulcer index, pepsin activity and gastric volume in IND-treated groups in respect to control group. Tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6) levels were elevated in IND-treated groups in respect to control due to withdrawal of prostaglandin and cyclooxygenase (COX-2) enzyme induced suppression. Significant protection in the levels of these ulcerogenic parameters were observed after the treatment of Ayapana triplinervis (200mg/kg) when compared with the standard drug omeprazole (20mg/kg). The important antioxidant enzymes activities were decreased but lipid peroxidation levels elevated in all the ulcerated groups(p<0.05). Gastricoccult test was positive in 40mg IND-treated group. Glutamate oxaloacetate transaminase (GOT) and glutamate pyruvate transaminase (GPT) activities in serum measured for toxicity assessment. All the parameters that altered in ulcerated groups were significantly protected towards the control level after Ayapana triplinervis or omeprazole treatment. This significant protective efficacy observed in histopathological evaluation of gastric tissue. Ayapana triplinervis significantly protect the mild, moderate and severe grade ulcers by its antioxidant activity and withdrawal of IND-induced suppression of prostaglandin and COX-2 levels.INTRODUCTION: Gastric ulcer (GU) is a major public health burden in the present century 1 with a high morbidity of about 5-10 %. Annually 200,000 people are hospitalized with the diagnosis of ulcer and the financial cost of the treatment of this disease reaches about 4 billion dollars 2 .

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Section: Discussionmentioning

confidence: 99%

“…Estimation of Gastric pH: After sacrifice, gastric content was collected and centrifuged for 10 min at 1000 rpm (4°C) then the clear supernatant was collected and used for the determination of pH by using pH meter 20 .…”

Section: Group Xmentioning

confidence: 99%

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2024

IJPSR

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“…It has been shown that HCl/EtOH stimulation results in activating NF-κB which leads to phosphorylation of IκBα and P50/P65 heterodimer, followed by translocation to the nucleus, thus prompting cells to secrete infammatory factors (e.g., TNF-α, IL-1β, and IL-6) and inducing an infammatory response [39]. Elevated levels of TNF-α could stimulate leukocytes to secrete more IL-1β and IL-6, thus causing even more potent infammatory response [40]. IL-1β is involved in ulcer formation [41], and elevated IL-1β exacerbates ulcer formation.…”

Section: Discussionmentioning

confidence: 99%

Protective Effects of Aqueous Extracts of the Herb of Paederia scandens (Lour.) Merr. against HCl/EtOH-Induced Gastric Ulcer in Rats: Involvement and Inhibitors’ Identification of NF-κB Signaling

Guo

Gong

et al. 2023

Journal of Food Biochemistry

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Paederia scandens (Lour.) Merr. (P. scandens), as an edible and medicinal herb, is commonly used for treating gastric ulcer in China; however, its mechanistic and effective substances’ studies remain limited. This study aimed to investigate the antigastric ulcer effects of aqueous extracts of P. scandens (PS-AE) and explore the involvement of NF-κB signaling in this effect. In addition, the study aimed to identify the effective substances by exploring NF-κB inhibitors. The main components of PS-AE were identified using an UPLC-MS method. HCl/EtOH-induced acute gastric ulcer in rats, and LPS-stimulated RAW264.7 cells were used to determine the antigastric ulcer effects of PS-AE in vivo and in vitro, respectively. Histopathological changes of the ulcer tissues were observed using staining methods of HE and PAS; secretions of inflammatory cytokines and oxidative stress indexes were measured using biochemical test kits; protein levels of NF-κB signaling pathway-related molecules were measured using Western Blot. NF-κB inhibitors’ identification in P. scandens was performed by developing HipHop pharmacophore model, and the results were further validated by molecular docking. In vivo, PS-AE significantly attenuated gastric ulcer in rats; reduced inflammation by adjusting the inflammatory cytokines TNF-α, IL-1β, and IL-6 levels; and induced oxidative stress can be alleviated via adjusting the levels of SOD, MDA, and GSH-Px. In addition, PS-AE may play an anti-inflammatory role mainly through NF-κB signaling pathway. Following pharmacophore modeling and molecular docking, 13 potential inhibitors targeting NF-κB were identified. In addition, in vitro, PS-AE and paederoside could significantly inhibit the activation of NF-κB p65 and the productions of downstream cytokines including NO, TNF-α, and IL-6. PS-AE protective effects on gastric ulcer damage induced by HCl/EtOH in rats, which is probably associated with its antioxidant effect and the inhibitory effects on levels of inflammatory mediators regulated by NF-κB signaling pathway. 13 potential NF-κB-targeting inhibitors are probably effective substances contributing to the herb’s pharmacological effect. In practical application, Paederia scandens, a traditional food and medicinal plant, possessed diversified chemical components and multiple pharmacological activities (anti-inflammatory, bacteriostasis, and antioxidative.). In China, P. scandens has been used as a traditional medicine to treat gastritis for hundreds of years, and it may be a potentially effective dietary product to improve gastric ulcer. This study shows that P. scandens exerts protective effects on gastric ulcer damage induced by HCl/EtOH in rats, which is probably associated with its antioxidant effect and the inhibitory effects on levels of inflammatory mediators regulated by NF-κB signaling pathway, which provides pharmacological justifications for the functional food use of P. scandens for treating gastric disease.

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“…The data were represented as mean ± SEM. The comparisons between the mean values of different groups were assessed by oneway analysis of variance (ANOVA), followed by multiple comparison two-tailed t test using SPSS software, version 16.0, which is installed in Microsoft Windows 10 operating system (Maity et al, 2021). A significant difference in means has been expressed at p < 0.05.…”

Section: Discussionmentioning

confidence: 99%

In vitro spermicidal effect of Thevetia Peruviana leaves on human spermatozoa

2021

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An in vitro spermicidal effect of aqua-methanolic (2:3) extract of Thevetia peruviana leaves on human spermatozoa was evaluated in a dose-dependent manner (20, 40, 80 and 160 mg/ml) at a 1:1 ratio. Sperm motility, viability, hypo-osmotic swelling (HOS) and acrosomal status and function tests were performed immediately (20 s), and after 5 and 10 min of exposure of the spermatozoa to the extract of Thevetia peruviana

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Gastroprotective effect of hydromethanolic extract of Ayapana triplinervis leaves on indomethacin‐induced gastric ulcer in male Wistar rats

Cited by 13 publications

References 52 publications

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Protective Effects of Aqueous Extracts of the Herb of Paederia scandens (Lour.) Merr. against HCl/EtOH-Induced Gastric Ulcer in Rats: Involvement and Inhibitors’ Identification of NF-κB Signaling

In vitro spermicidal effect of Thevetia Peruviana leaves on human spermatozoa

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