Gastroprotective Effect of Zingerone on Ethanol-Induced Gastric Ulcers in Rats

Karampour, Neda Sistani; Arzi, Ardeshir; Rezaie, Annahita; Pashmforoosh, Marzieh; Kordi, Fatemeh

doi:10.3390/medicina55030064

Cited by 78 publications

(51 citation statements)

References 31 publications

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“…The enhanced secretion of gastric mucus noted in SA- and omeprazole-pre-treated rats could be attributed to the increased production of PGE 2 and NO in the gastric mucosa. The findings of the present study are consistent with those of many previous reports, which have provided evidence that SA has anti-secretagogue, anti-ulcer, and gastroprotective activity in ethanol-induced GUs in rats ( Sistani Karampour et al, 2019 ; Zhou et al, 2020 ). The inflammatory response is a key process in the gastric mucosa defense mechanism ( Martin and Wallace, 2006 ).…”

Section: Discussionsupporting

confidence: 93%

“…In this research, an 80% ethanol (5 ml/kg)-induced gastric ulcer model was established. The ethanol-induced gastric ulcer experimental model represents many aspects of the disorder of human gastric ulceration and is, thus, useful in determining the anti-ulcer capacity of drugs, as well as the likely pathways involved in this process ( Kim et al, 2005 ; Huang et al, 2013 ; Vijayakumar et al, 2016 ; Sistani Karampour et al, 2019 ). In addition, ethanol is one of the most frequent causes of gastric ulceration.…”

Section: Discussionmentioning

confidence: 99%

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Gastroprotective Effect of Sinapic Acid on Ethanol-Induced Gastric Ulcers in Rats: Involvement of Nrf2/HO-1 and NF-κB Signaling and Antiapoptotic Role

et al. 2021

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Background: In the current study, we evaluated the therapeutic potential of sinapic acid (SA) in terms of the mechanism underlying its gastroprotective action against ethanol-induced gastric ulcers in rats.Methods: These effects were examined through gross macroscopic evaluation of the stomach cavity [gastric ulcer index (GUI)], alteration in pH, gastric juice volume, free acidity, total acidity, total gastric wall mucus, and changes in PGE2. In addition, we evaluated lipid peroxidation (malondialdehyde), antioxidant systems (catalase and glutathione), inflammatory markers [tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), and myeloperoxidase (MPO)], apoptotic markers (caspase-3, Bax, and Bcl-2), nuclear factor-κB [NF-κB (p65)], NO levels, and histopathological staining (H and E and PAS).Results: In rats with ethanol-induced ulcers, pre-treatment with SA (40 mg/kg p. o.) decreased the sternness of ethanol-induced gastric mucosal injuries by decreasing the GUI, gastric juice volume, free acidity, and total acidity. In addition, the pH and total gastric mucosa were increased, together with histopathological alteration, neutrophil incursion, and increases in PGE2 and NO2. These effects were similar to those observed for omeprazole, a standard anti-ulcer drug. SA was shown to suppress gastric inflammation through decreasing TNF-α, IL-6, and MPO, as well as curbing gastric oxidative stress through the inhibition of lipid peroxidation (MDA) and restoration of depleted glutathione and catalase activity. SA inhibited Bcl-2-associated X (Bax) and caspase-3 activity, and restored the antiapoptotic protein Bcl-2; these findings indicate the antiapoptotic potential of SA, leading to enhanced cell survival. SA also repressed NF-κB signaling and increased IκBα. Moreover, SA upregulated the nuclear factor erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1), thereby restoring depleted antioxidant defense enzymes and implicating the NRF2/HO-1 signaling pathways.Conclusion: These results suggest that the prophylactic administration of SA (40 mg/kg) can ameliorate ethanol-induced gastric ulcers in rats primarily via the modulation of Nrf2/HO-1 and NF-κB signaling and subsequent enhancement of cell viability.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Gastroprotective Effect of Sinapic Acid on Ethanol-Induced Gastric Ulcers in Rats: Involvement of Nrf2/HO-1 and NF-κB Signaling and Antiapoptotic Role

et al. 2021

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“…The results were promising, where nearly normal jejunal architecture were detected in ZO alcohol group with slight affection of the jejunal mucosa in the form of few areas of ulceration of the top of some villi and few enterocytes showed loss of their covering brush microvillous border. These findings were in agree with Sistani et al ,[ 8 ] who stated that oral pretreatment with ZO had decreased the number and the length of ethanol induced gastric ulcers.…”

Section: Discussionsupporting

confidence: 93%

The possible protective role of zingerone on ethanol induced entrotoxicity of jejunum in adult albino rats: Light and scanning electron microscopic study

Amer

2020

J Microsc Ultrastruct

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Introduction: Zingerone is a nontoxic important extract of dry ginger plant. It is reported that zingerone has an anticancer property, strong anti-inflammatory and antimicrobial properties. Aim of the Work: is to evaluate the possible protective effects of zingerone on ethanol-induced lesions on the jejunum of adult male albino rats. Materials and Methods: twenty four adult male albino rats were used, divided into 3 groups; A control group (I); consisted of 8 rats, ethanol group (II); contained 8 rats and each rat given 50% v/v alcohol at a dose of 4 g/kg.bw orally for 15 days. Ethanol zingerone group (III); consisted of 8 rats, each received zingerone at a dose of 50 mg/kg and alcohol at the same previous dose daily and orally for 15 days. At the appropriate time, the specimens were taken and prepared for light and electron microscope study. Results: Histological examination of jejunum sections of ethanol group (II) showed massive jejunal villi ulcerations with shedding of their surface epithelium, loss of the villous architecture and loss of the microvilli covering some enterocytes. Examination of ethanol zingerone group (III) showed evidence of improvement in the form of nearly normal architecture of the jejunal villi with few areas of ulcerations on the top of some villi and increased cells with mitotic activity. Conclusion: Accordingly, we can conclude that zingerone administration can remarkably ameliorate ethanol-induced enterotoxiciy and jejunal ulcerations in rats by its anti-inflammatory properties and by suppressing oxidative stress.

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“…One of the most important causes of the establishment and development of gastric ulcer in humans is ethanol, which is why gastric ulcer caused by the administration of ethanol in rats is a model essential for the preclinical study of new potentially antiulcer substances [ 31 ]. After administration of ethanol in rats, there are significant necrotic lesions and cell infiltration with significant reduction of defense factors (production of mucus, bicarbonate, and circulation of the mucous membranes) of the stomach wall [ 32 – 34 ]. However, it is known that one of the important elements in the protection of the stomach wall against multiple gastric lesions is the secretion of mucus [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

Methanolic Extract of Distemonanthus benthamianus (Caesalpiniaceae) Stem Bark Suppresses Ethanol/Indomethacin-Induced Chronic Gastric Injury in Rats

Marte

Ateufack

Mbiantcha

et al. 2020

Gastroenterology Research and Practice

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Distemonanthus benthamianus (Caesalpiniaceae) is a plant from the Cameroon pharmacopoeia very widely used in the treatment of many pathologies among which are gastrointestinal disorders. The main purpose of this study was to assess the healing properties of gastric ulcer from the methanolic extract of Distemonanthus benthamianus and its mechanisms of action. The healing properties of gastric ulcers (chronic ulcer model induced by ethanol and indomethacin) were evaluated in vivo in adult male rats, while the mechanisms of action were evaluated in vitro by anti-inflammatory assay (protein denaturation, cyclooxygenase, and lipoxygenase assays) and immunomodulatory assay (ROS production (using technical chemiluminescence), cytokine (TNF-α, IL-1β, IL-6) production (using ELISA), proliferation of T cells (using liquid scintillation counter), and cytotoxicity (using MTT assay)). The methanolic extract of Distemonanthus benthamianus inhibited protein denaturation (75.63%) and the activities of cyclooxygenase (78.92%) and 5-lipoxygenase (81.54%). The extract also significantly ( p < 0.001 ) inhibited intracellular and extracellular ROS production and T cell proliferation and reduced significantly ( p < 0.01 , p < 0.001 ) TNF-α, IL-1β, IL-6, and PGE2 production. At all doses (125, 250, and 500 mg/kg), the extract significantly reduces the ulceration index and the area of ulceration and significantly increases the mass of gastric mucus. In addition, the extract significantly decreases the level of MDA, significantly increases the activities of catalase and glutathione, and then improves the hematological parameters in sick animals. Histological micrographs show that in the presence of the extract, there is advanced reepithelialization with recovery of the ulcerated epithelium. Thus, the extract of Distemonanthus benthamianus has healing properties against gastric ulcers which are associated with its anti-inflammatory, immunomodulatory, and antioxidant effects.

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Gastroprotective Effect of Zingerone on Ethanol-Induced Gastric Ulcers in Rats

Cited by 78 publications

References 31 publications

Gastroprotective Effect of Sinapic Acid on Ethanol-Induced Gastric Ulcers in Rats: Involvement of Nrf2/HO-1 and NF-κB Signaling and Antiapoptotic Role

Gastroprotective Effect of Sinapic Acid on Ethanol-Induced Gastric Ulcers in Rats: Involvement of Nrf2/HO-1 and NF-κB Signaling and Antiapoptotic Role

The possible protective role of zingerone on ethanol induced entrotoxicity of jejunum in adult albino rats: Light and scanning electron microscopic study

Methanolic Extract of Distemonanthus benthamianus (Caesalpiniaceae) Stem Bark Suppresses Ethanol/Indomethacin-Induced Chronic Gastric Injury in Rats

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