Gastrulation: Its Principles and Variations

Kondoh, Hisato

doi:10.1007/978-3-031-39027-2_3

Results and Problems in Cell Differentiation

2024

DOI: 10.1007/978-3-031-39027-2_3

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Gastrulation: Its Principles and Variations

Hisato Kondoh

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Chicken embryo cultures in the dorsal‐upward orientation for the manipulation of epiblasts

Konya,

Watanabe,

Kawamura

et al. 2024

Dev Growth Differ

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Chicken embryos have many advantages in the study of amniote embryonic development. In particular, culture techniques developed for early‐stage embryos have promoted the advancement of modern developmental studies using chicken embryos. However, the standard technique involves placing chicken embryos in the ventral‐upward (ventral‐up) orientation, limiting manipulation of the epiblast at the dorsal surface, which is the primary source of ectodermal and mesodermal tissues. To circumvent this limitation, we developed chicken embryo cultures in the dorsal‐up orientation and exploited this technique to address diverse issues. In this article, we first review the history of chicken embryo culture techniques to evaluate the advantages and limitations of the current standard technique. Then, the dorsal‐up technique is discussed. These technological discussions are followed by three different examples of experimental analyses using dorsal‐up cultures to illustrate their advantages: (1) EdU labeling of epiblast cells to assess potential variation in the cell proliferation rate; (2) migration behaviors of N1 enhancer‐active epiblast cells revealed by tracking cells with focal fluorescent dye labeling in dorsal‐up embryo culture; and (3) neural crest development of mouse neural stem cells in chicken embryos.

show abstract

Chicken embryo cultures in the dorsal‐upward orientation for the manipulation of epiblasts

Konya,

Watanabe,

Kawamura

et al. 2024

Dev Growth Differ

View full text Add to dashboard Cite

show abstract

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Gastrulation: Its Principles and Variations

Cited by 1 publication

References 76 publications

Chicken embryo cultures in the dorsal‐upward orientation for the manipulation of epiblasts

Chicken embryo cultures in the dorsal‐upward orientation for the manipulation of epiblasts

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