GATA3 induces the upregulation of UCP-1 by directly binding to PGC-1α during adipose tissue browning

Son, Min Jeong; Oh, Kyoung-Jin; Park, Anna; Kwon, Min‐Gi; Suh, Jae Myoung; Kim, Il-Chul; Kim, Se-Yun; Lee, Sang Chul; Kim, Won Kon; Bae, Kwang‐Hee

doi:10.1016/j.metabol.2020.154280

Cited by 17 publications

(6 citation statements)

References 53 publications

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“…Interestingly, mitochondrial reactive oxygen species could induce the activation of primary human T cells by regulating IL-2 and IL-4 expression [ 60 ]. GATA3, a cell-specific marker of TH2 cells, could modulate mitochondrial biogenesis and increase the mitochondrial content [ 61 , 62 ]. The abovementioned studies show that mitochondrial regulation, cell metabolism, and oxidative stress may play certain roles in the differentiation and activation of TH2 cells, which may be related to the difference in TH2 cell infiltration between the high- and low-risk groups.…”

Section: Discussionmentioning

confidence: 99%

Mitochondria-Related Transcriptome Characterization Associated with the Immune Microenvironment, Therapeutic Response and Survival Prediction in Pancreatic Cancer

Dong

Liu

Zhang

et al. 2023

IJMS

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(1) Background: Pancreatic cancer (PC) is one of the most lethal tumors. Mitochondrial dysfunction has been reported to be involved in cancer development; however, its role in PC has remained unclear. (2) Methods: The differentially expressed NMGs were selected between PC and normal pancreatic tissue. The NMG-related prognostic signature was established by LASSO regression. A nomogram was developed based on the 12-gene signature combined with other significant pathological features. An extensive analysis of the 12 critical NMGs was performed in multiple dimensions. The expression of some key genes was verified in our external cohort. (3) Results: Mitochondria-related transcriptome features was obviously altered in PC compared with normal pancreas tissue. The 12-NMG signature showed good performance in predicting prognosis in various cohorts. The high- and low-risk groups exhibited notable diversity in gene mutation characteristics, biological characteristics, chemotherapy response, and the tumor immune microenvironment. Critical gene expression was demonstrated in our cohort at the mRNA and protein levels and in organelle localization. (4) Conclusions: Our study analyzed the mitochondrial molecular characterization of PC, proving the crucial role of NMGs in PC development. The established NMG signature helps classify patient subtypes in terms of prognosis prediction, treatment response, immunological features, and biological function, providing a potential therapeutic strategy targeting mitochondrial transcriptome characterization.

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Section: Discussionmentioning

confidence: 99%

Mitochondria-Related Transcriptome Characterization Associated with the Immune Microenvironment, Therapeutic Response and Survival Prediction in Pancreatic Cancer

Dong

Liu

Zhang

et al. 2023

IJMS

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“…Evidence for GATA3 being a metabolic regulator is growing. Son et al recently demonstrated that GATA3 directly binds to PGC1α to regulate thermogenesis in white adipose tissue via increased UCP-1 expression 33 . We demonstrate here that in CD4 + T cells, PGC1α is activated by both GATA3 and AMPK.…”

Section: Discussionmentioning

confidence: 99%

GATA3 induces mitochondrial biogenesis in primary human CD4+ T cells during DNA damage

et al. 2021

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GATA3 is as a lineage-specific transcription factor that drives the differentiation of CD4+ T helper 2 (Th2) cells, but is also involved in a variety of processes such as immune regulation, proliferation and maintenance in other T cell and non-T cell lineages. Here we show a mechanism utilised by CD4+ T cells to increase mitochondrial mass in response to DNA damage through the actions of GATA3 and AMPK. Activated AMPK increases expression of PPARG coactivator 1 alpha (PPARGC1A or PGC1α protein) at the level of transcription and GATA3 at the level of translation, while DNA damage enhances expression of nuclear factor erythroid 2-related factor 2 (NFE2L2 or NRF2). PGC1α, GATA3 and NRF2 complex together with the ATR to promote mitochondrial biogenesis. These findings extend the pleotropic interactions of GATA3 and highlight the potential for GATA3-targeted cell manipulation for intervention in CD4+ T cell viability and function after DNA damage.

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“…Seperti halnya peptida dari fraksi kefir dengan BM (Berat Molekul) > 3 kD, 3-30kD dan < 3 kD dapat menghambat diferensiasi sel adiposit 3T3-L1 [40]. Pemberian peptida kokon ulat sutra (Bombyx mori cocoons) (1 mg/mL) dapat menghambat TG (86,10%) dibanding kontrol, melalui mekanisme meningkatkan sensitifitas insulin dan penyerapan glukosa pada adiposit 3T3-L1 [61] dan aktivasi protein AMP-kinase pada tikus dengan HFD [62] [3].…”

Section: Pengaruh Pemberian Whey-ksk Terhadap Total Tgunclassified

Potensi Whey Kefir Susu Kambing Sebagai Anti-Obesitas Melalui Penghambatan Sintesis Lipid dan Aktivitas Phosphoenolpyruvate Carboxykinase (PEPCK) pada Sel Model Adiposit 3T3-L1

Radiati

Hati

Andarini

et al. 2022

IJHN

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Obesitas menjadi salah satu penyebab utama sindrom metabolik dislipidemia, yang dapat sebagai komorbid berbagai penyakit. Penggunaan obat-obatan untuk mengurangi obesitas memiliki akibat yang merugikan, oleh karena itu dikembangkan produk komplementer dari susu fermentasi sebagai strategi non-farmakologis untuk pengelolaan dislipidemia. Pemecahan masalah obesitas dapat dilakukan melalui pendekatan adipogenesis pada sel model adiposit 3T3-L1. Tujuan penelitian ini adalah menganalisis pemberian whey-KSK terhadap TG (Total Trigliserida), TC (Total Kolesterol) dan aktivitas PEPCK (Phosphoenol pyruvate Carboxykinase) sel adiposit 3T3-L1. Metode penelitian adalah percobaan pemberian dosis whey-KSK yang berbeda yaitu P1 (25mg/ml), P2 (50 mg/ml), P3 (75 mg/ml), P4 (100 mg/ml), dan kelompok KN (kontrol negatif) dan KP (kontrol positif) pada adiposit 3T3-L1, dengan empat kali ulangan. Hasil penelitian menunjukkan whey-KSK 25 – 100 µg/mL dapat menurunkan TG sebesar 35,39 – 55,32%, menurunkan TC sebesar 30,46-62,12%, menurunkan aktivitas PEPCK sebesar 27,10-82,52% dan menurunkan aktivitas spesifik PEPCK sebesar 33,06-63,34%. Kesimpulan whey-KSK dapat menghambat adipogenesis sel adiposit 3T3-L1 dan berpotensi sebagai antiobesitas.

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GATA3 induces the upregulation of UCP-1 by directly binding to PGC-1α during adipose tissue browning

Cited by 17 publications

References 53 publications

Mitochondria-Related Transcriptome Characterization Associated with the Immune Microenvironment, Therapeutic Response and Survival Prediction in Pancreatic Cancer

Mitochondria-Related Transcriptome Characterization Associated with the Immune Microenvironment, Therapeutic Response and Survival Prediction in Pancreatic Cancer

GATA3 induces mitochondrial biogenesis in primary human CD4+ T cells during DNA damage

Potensi Whey Kefir Susu Kambing Sebagai Anti-Obesitas Melalui Penghambatan Sintesis Lipid dan Aktivitas Phosphoenolpyruvate Carboxykinase (PEPCK) pada Sel Model Adiposit 3T3-L1

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