2020
DOI: 10.3389/fmed.2020.00044
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GATA4 Is Required for Budding Morphogenesis of Posterior Foregut Endoderm in a Model of Human Stomach Development

Abstract: Three-dimensional gastrointestinal organoid culture systems provide innovative and tractable models to investigate fundamental developmental biology questions using human cells. The goal of this study was to explore the role of the zinc-finger containing transcription factor GATA4 in gastric development using an organoid-based model of human stomach development. Given GATA4 ′ s vital role in the developing mouse gastrointestinal tract, we hypothesized that GATA4 plays an essential role in human stomach develop… Show more

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Cited by 6 publications
(9 citation statements)
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References 36 publications
(75 reference statements)
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“…A board-certified GI pathologist verified the presence of Barrett's esophagus or esophageal adenocarcinoma in tissues. Standard immunohistochemical procedures were followed 41 , 48 , 49 . Briefly, citric acid antigen retrieval was performed before immunohistochemistry for GATA4 (R&D Systems, AF2606).…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…A board-certified GI pathologist verified the presence of Barrett's esophagus or esophageal adenocarcinoma in tissues. Standard immunohistochemical procedures were followed 41 , 48 , 49 . Briefly, citric acid antigen retrieval was performed before immunohistochemistry for GATA4 (R&D Systems, AF2606).…”
Section: Methodsmentioning
confidence: 99%
“…Standard immunocytochemistry methods were used 49 . Briefly, cells were fixed with 4% PFA/1X PBS, permeabilized with 0.5% Triton X-100/1X PBS, blocked with 3% BSA/1X PBS, and incubated with antibodies (GATA4, R&D Systems, AF2606; Alexa-Fluor 594 Donkey anti-goat, Thermo Fisher) in 1% BSA/1X PBS.…”
Section: Methodsmentioning
confidence: 99%
“…Standard immunohistochemical procedures were followed. [32,45] Briefly, citric acid antigen retrieval was performed prior to immunohistochemistry for GATA4 (R&D Systems, AF2606). Staining was visualized using R.T.U.…”
Section: Tissue Immunohistochemistrymentioning
confidence: 99%
“…Standard immunocytochemistry methods were used. [45] Briefly, cells were fixed with 4% PFA/1X PBS, permeabilized with 0.5% Triton X-100/1X PBS, blocked with 3% BSA/1X PBS, and incubated with antibodies (GATA4, R&D Systems, AF2606; Alexa-Fluor 594 Donkey anti-goat, Thermo Fisher) in 1% BSA/1X PBS. DAPI was used to visualize nuclei.…”
Section: Immunocytochemistrymentioning
confidence: 99%
See 1 more Smart Citation