2012
DOI: 10.1038/nature10752
|View full text |Cite
|
Sign up to set email alerts
|

Gated regulation of CRAC channel ion selectivity by STIM1

Abstract: Two defining functional features of ion channels are ion selectivity and channel gating. Ion selectivity is generally considered an immutable property of the open channel structure, whereas gating involves transitions between open and closed channel states typically without changes in ion selectivity 1. In store-operated Ca2+ release-activated Ca2+ (CRAC) channels, the molecular mechanism of channel gating by the CRAC channel activator, STIM1 (stromal interaction molecule 1) remains unknown. CRAC channels are … Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
4
1

Citation Types

17
244
1

Year Published

2014
2014
2020
2020

Publication Types

Select...
4
2
1

Relationship

0
7

Authors

Journals

citations
Cited by 193 publications
(262 citation statements)
references
References 30 publications
17
244
1
Order By: Relevance
“…In silico calculations for the Drosophila V174A channel—corresponding to a human V102A channel—suggest that it retains the closed pore conformation of the wildtype Drosophila channel but presents a markedly lower energetic barrier to ion conductance (Dong et al 2013). Consistent with the idea that the channel is not already in the STIM-gated conformation, the relatively nonselective V102X channels undergo a STIM-dependent conformational change to more selective channels (McNally et al 2012; Derler et al 2013). The Tb 3+ luminescence assay also detects a STIM-dependent conformational change in the V102A channel (Gudlur et al 2014).…”
Section: 7 Channel Gatingsupporting
confidence: 63%
See 3 more Smart Citations
“…In silico calculations for the Drosophila V174A channel—corresponding to a human V102A channel—suggest that it retains the closed pore conformation of the wildtype Drosophila channel but presents a markedly lower energetic barrier to ion conductance (Dong et al 2013). Consistent with the idea that the channel is not already in the STIM-gated conformation, the relatively nonselective V102X channels undergo a STIM-dependent conformational change to more selective channels (McNally et al 2012; Derler et al 2013). The Tb 3+ luminescence assay also detects a STIM-dependent conformational change in the V102A channel (Gudlur et al 2014).…”
Section: 7 Channel Gatingsupporting
confidence: 63%
“…A circumstantial case could be made that the SCID mutation blocks ion flux through an otherwise open channel in store-depleted cells. The R91W tryptophan ring is seen to occlude the channel in the Drosophila Orai crystal structure (Hou et al 2012), and experimentally the R91W replacement overrides the constitutive conductance of the V102C mutant (McNally et al 2012). However, both observations refer to the closed conformation of the channel.…”
Section: 7 Channel Gatingmentioning
confidence: 99%
See 2 more Smart Citations
“…The Orai channel protein has four transmembrane helices and crystallography reveals the channel to be a hexamer [16]. The N-terminal helices of each of the six Orai subunits form the central Orai channel pore [1720]. The Orai1 cytoplasmic N- and C-termini extend into the cytosol in close proximity to each other [16, 21].…”
Section: Introductionmentioning
confidence: 99%