The most valuable information of Hyperspectral Image (HSI) should be processed properly. Using dimensionality reduction techniques in two distinct approaches, we created a structure for HSI to collect physiological and diagnostic information. The tissue Oxygen Saturation Level (StO2) was extracted using the HSI approach as a physiological characteristic for stress detection. Our research findings suggest that this unique characteristic may not be affected by humidity or temperature in the environment. Comparing the standard StO2 reference and pressure concentrations, the social stress assessments showed a substantial variance and considerable practical differentiation. The proposed system has already been evaluated on tumor images from rats with head and neck cancers using a spectrum from 450 to 900 nm wavelength. The Fourier transformation was developed to improve precision, and normalize the brightness and mean spectrum components. The analysis of results showed that in a difficult situation where awareness could be inexpensive due to feature possibilities for rapid classification tasks and significant in measuring the structure of HSI analysis for cancer detection throughout the surgical resection of wildlife. Our proposed model improves performance measures such as reliability at 89.62% and accuracy at 95.26% when compared with existing systems.