We study the Hankel determinant generated by the Laguerre weight with jump discontinuities at t k , k = 1, • • • , m. By employing the ladder operator approach to establish Riccati equations, we show that σ n (t 1 , • • • , t m ), the logarithmic derivative of the n-dimensional Hankel determinant, satisfies a generalization of the σ-from of Painlevé V equation. Through investigating the Riemann-Hilbert problem for the associated orthogonal polynomials and via Lax pair, we express σ n in terms of solutions of a coupled Painlevé V system. We also build relations between the auxiliary quantities introduced in the above two methods, which provides connections between the Riccati equations and Lax pair. In addition, when each t k tends to the hard edge of the spectrum and n goes to ∞, the scaled σ n is shown to satisfy a generalized Painlevé III equation.