Gavage approach to oxygen supplementation with oxygen therapeutic Ox66™ in a hypoventilation rodent model of respiratory distress

Nugent, William H.; Carr, Danuel A; MacBryde, Rosa; Bruce, Erica D.; Song, Bjorn K.

doi:10.1080/21691401.2021.2013251

Cited by 4 publications

(1 citation statement)

References 32 publications

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“…Measurements of the partial pressure of oxygen in interstitial fluid (P ISF O 2 ) of the exteriorized spinotrapezius muscle and the technical setup have been previously described in depth (13)(14)(15). Briefly, a phosphor (Oxyphore R0; Frontier Scientific, Newark, DE) bound to albumin (Bovine Serum Albumin; Sigma Aldrich, St Louis, MO) was topically diffused into the spinotrapezius' interstitium.…”

Section: Phosphorescence Quenching Microscopymentioning

confidence: 99%

Exchange Transfusion With vs-101: A New Pegylated-Hb Designed to Restore Perfusion and Increase O2 Carrying Capacity

Nugent,

Sheppard,

Vandegriff

et al. 2023

Shock

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Blood products are the current standard for resuscitation of hemorrhagic shock. However, logistical constraints of perishable blood limit availability and pre-hospital use meaning alternatives that provide blood-like responses remain an area of active investigation and development. VS-101 is a new PEGylated human hemoglobin-based oxygen carrier that avoids the logistical hurdles of traditional blood transfusion. This study sought to determine the safety and ability of VS-101 to maintain circulatory function and capillary oxygen delivery in a severe (50%) exchange transfusion (ET) model. Anesthetized, male Sprague Dawley rats were prepared for cardiovascular monitoring and phosphorescence quenching microscopy of interstitial fluid oxygen tension (PISFO2) in the spinotrapezius muscle. 50% isovolemic ET of estimated total blood volume with either Lactated Ringer’s (LRS; N = 8) or VS-101 (N = 8) at 1 mL/kg/min was performed, and animals were observed for 240 min. VS-101 maintained PISFO2 at baseline (BL) with a transient 18 ± 4 mmHg decrease (p < 0.05) in mean arterial pressure (MAP). In contrast, ET with LRS decreased PISFO2 by ~50% (p < 0.05) and MAP by 74 ± 10 mmHg (p < 0.05). All VS-101 animals survived 240 min, the experimental endpoint, while 100% of LRS animals expired by 142 min. VS-101 animals maintained normal tissue oxygenation through 210 min, decreasing by 25% (p < 0.05 vs. BL) thereafter, likely from VS-101 vascular clearance. No arteriolar vasoconstriction was observed following VS-101 treatment. In this model of severe exchange transfusion, VS-101 effectively maintained blood pressure, perfusion, and PISFO2 with no vasoconstrictive effects. Further elucidation of these beneficial resuscitation effects of VS-101 is warranted to support future clinical trials.

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Section: Phosphorescence Quenching Microscopymentioning

confidence: 99%