GB Virus C (GBV-C) / Hepatitis G Virus (HGV): Towards the Design of Synthetic Peptides-based Biosensors for Immunodiagnosis of GBV-C / HGV Infection

Abstract

In the present study, new putative epitopes located in structural (E2) and non-structural (NS3) proteins of GBV-C/HGV were identified by computer-aided prediction of antigenicity and synthesized in solid-phase, following an Fmoc/tBut strategy, for their use in immunoassays. The corresponding synthetic peptides were used as antigens in ELISA assays and in real-time biospecific interaction measurements. This last approach allowed direct detection of GBV-C/HGV-specific antibodies in human sera. Good correlations were obtained between the biospecific interaction analysis and the ELISA. To verify the performance of these new assays in comparison to the existing recombinant E2 protein commercial test, antibodies to synthetic peptides were searched for in different panels of serum samples. The main conclusion of this work is the usefulness of E2 peptides in the detection of antibodies. Moreover, the NS3 peptide could be exploited to improve the sensitivity of the currently available test. Our results offer a new approach to develop new diagnostic peptide based biosensors for serodiagnosis of GBV-C/HGV infection.

Liposome destabilization induced by synthetic lipopeptides corresponding to envelope and non-structural domains of GBV-C/HGV virus. Conformational requirements for leakage

“…This sequence was able, on one hand, to bind with high affinity to negatively charged membranes modifying the biophysical properties of phospholipid model membranes and, on t he other hand, to permeabilize efficiently negatively charged vesicles [20]. In our group, the membrane-interacting properties of two epitopes of GBV-C/HGV located, respectively, at the regions (99-118) of the E2 envelope protein and (440-460) of the NS3 non-structural protein and their lipoderivatives were recently studied [21]. We reported a higher affinity binding of parent and lipophilic envelope E2 peptides than non-structural NS3 peptides to model membranes composed of DPPC [16,22].…”

Use of [Cit312,314] filaggrin (306–324) analogue for the diagnosis of rheumatoid arthritis. Conformational study by circular dichroism and fourier transformed infrared spectroscopy

“…In the present work and as we previously reported [13,17] to overcome the low coating efficiency of highly hydrophilic linear peptides to microtiter plates in the ELISA and in order to avoid unpredictable orientation of the peptides on the surface, the hydrophobic derivatization of linear sequences by the introduction of a palmitic tail at the N-terminus was carried out. Moreover, the covalent binding of filaggrin belonging synthetic peptides to preactivated as N-oxisuccinimidyl active ester plates, that orients the immobilized molecule on the surface in a specific manner (covalent immobilization involving the peptide amino groups) was also assayed.…”

Optimization of surface plasmon resonance based biosensor for clinical diagnosis of the Epstein–Barr herpes virus disease

“…V. 19, N 1. P. 84-89. doi: 10.15407/spqeo19.01.084 important fields of SPR method application is rapid and accurate detection of viral agents and antibodies in blood serum [5][6][7]. The Epstein-Barr virus (EBV, 4 th type human herpevirus) is one of the most widespread and dangerous human viruses, named after the English virologist prof. Michael Anthony Epstein and his graduate student Yvonne Barr, who described the virus in 1964 [8].…”