GC-FID phytochemical analysis of selected antidiabetic herbal preparations sold in Onitsha metropolis, Anambra state, Nigeria

Ojukwu, Stephen Udoka; Onyegbule, FA; Ce, Umeyor

doi:10.22271/plants.2021.v9.i4a.1313

Cited by 3 publications

(2 citation statements)

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“…Ojukwu et al 35 . As 15 mL ethanol and 10 mL 50% w/v KOH was added to a test tube containing one (1 g) of sample and the test tube was allowed to sit in a water bath at 60°C for 1 hr.…”

Section: Extraction Of Chemical Constituents: Extraction Of Chemical ...mentioning

confidence: 99%

In vitro Antidiabetic Studies of Aqueous Extract of Pleurotus ostreatus Grown on Different Substrates

Chukwurah,

Nnemolisa,

Chibuogwu

et al. 2023

Res. J. Phytochem

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Background and Objective: Nature-derived products are finding increased application for therapeutic purposes. Apart from their use as food, mushrooms have been reported in various research to contain bioactive compounds with medicinal capabilities. This study evaluated the in vitro antidiabetic and antioxidant activities of an aqueous extract of Pleurotus ostreatus (mushroom) cultivated on rice bran/sawdust (RB/SD, 70:30 w/w) mixture and sawdust (SD) substrates. Materials and Methods: Pleurotus ostreatus was cultivated on two different substrates followed by aqueous extraction of mature mushrooms.In vitro antidiabetic, (α-amylase and α-glucosidase inhibition) and antioxidant (DPPH radical-scavenging assay and ferric reducing power (FRAP) activities were done using standard methods. Results were analyzed using IBM Statistical Product and Service Solutions (SPSS) software, version 23 and the significance level was established at p<0.05. Results: The extracts showed a concentration-dependent inhibitory effect on α-amylase and α-glucosidase activities in vitro. There was a stronger inhibition of α-amylase activity by the RB/SD sample when compared to SD and a weaker inhibitory effect of the RB/SD sample on α-glucosidase activity when compared to SD. Again, the samples showed a concentration-dependent antioxidant effect with increased DPPH-scavenging activity and FRAP observed with the SD sample compared to the RB/SD sample. The GC-FID analysis of the P. ostreatus samples also revealed the presence of a number of bioactive compounds in different concentrations. Conclusion: These results highlight the effect of cultivation substrate on the potential therapeutic effect of mushrooms the aqueous extract of P. ostreatus could be a promising raw material for the development of anti-diabetes therapeutics.

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“…Ojukwu et al 35 . As 15 mL ethanol and 10 mL 50% w/v KOH was added to a test tube containing one (1 g) of sample and the test tube was allowed to sit in a water bath at 60°C for 1 hr.…”

Section: Extraction Of Chemical Constituents: Extraction Of Chemical ...mentioning

confidence: 99%

In vitro Antidiabetic Studies of Aqueous Extract of Pleurotus ostreatus Grown on Different Substrates

Chukwurah,

Nnemolisa,

Chibuogwu

et al. 2023

Res. J. Phytochem

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“…Tannins have been also reported to possess antibacterial activity 18 . Sparteine is a quinoline alkaloid that possesses antimicrobial and antimalarial properties and it has been reported that both proanthocynin and spartein have antibacterial activity 19 . Sparteine has been studied to possess antimicrobial activity 20 .…”

Section: Antioxidant Activitymentioning

confidence: 99%

Antimicrobial and Antioxidant Activities of Secondary Metabolites of an Endophytic fungus of Azadirachta indica

Okonkwo-Uzor

Obi

Enwelum

et al. 2022

J. Drug Delivery Ther.

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Background: Recent upsurge in the mortality rate and long hospital stays as a result of antimicrobial resistance caused by multi-drug resistant bacteria is a huge burden to public health sector. Intensive search for more effective and newer agents to deal with these problems. Endophytes are a novel source of potentially useful medicinal compounds. Aim: The study aimed at evaluating the antimicrobial and antioxidant activities of an endophytic fungus isolated from Azadirachta indica against multi drug resistant bacteria species. Method: Endophytic fungus was isolated from fresh leaves of Azadirachta indica. The fungus was fermented in rice medium, and the secondary metabolites were extracted. The antimicrobial activity of the extract against laboratory strains of Staphylococcus aureus, Escherichia coli, Klebsiella pneumonia and Bacillus subtilis, Candida albicans, Aspergillus niger) was determined. The fungal extract was also evaluated for antioxidant activity using the DPPH assay method. Using GC-FID analysis, some constituents of the fungal extracted were identified. Result: At 1 and 0.5 mg/mL, the fungal extract demonstrated antibacterial activity against Klebsiella pneumoniae but no activity against the other test microbes. However, the fungal extract of A. indica showed excellent antioxidant activity due to possession of bioactive secondary metabolites. Conclusion: The result of GC-FID showed the presence of Rutin, Lunamarin, Anthocyanin, Catechin, Naringenin, Flavan 3 ol, Sapogernin, Tannin, Spartein, Naringin, Flavonones, Steroids, Kaemferol, Cyanogenic glycoside, Phytate, Epicatechin, Reseveratol, Cardic glycoside and Epihedrine at different concentrations. This study reveals the dynamic potentials of the endophytic fungus of A. indica as the bedrock of naturally occurring bioactive agent. Keywords: Azadirachta indica, endophytes, GC-FID, secondary metabolites, medicinal plants, phytochemicals.

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Antidiabetic and antioxidant potentials of Pleurotus ostreatus -derived compounds: An in vitro and in silico approach

Nnemolisa,

Chukwurah,

Edeh

et al. 2024

Food Chemistry Advances

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GC-FID phytochemical analysis of selected antidiabetic herbal preparations sold in Onitsha metropolis, Anambra state, Nigeria

Cited by 3 publications

References 40 publications

In vitro Antidiabetic Studies of Aqueous Extract of Pleurotus ostreatus Grown on Different Substrates

In vitro Antidiabetic Studies of Aqueous Extract of Pleurotus ostreatus Grown on Different Substrates

Antimicrobial and Antioxidant Activities of Secondary Metabolites of an Endophytic fungus of Azadirachta indica

Antidiabetic and antioxidant potentials of Pleurotus ostreatus -derived compounds: An in vitro and in silico approach

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