GC/MS analysis and potential synergistic effect of mandarin and marjoram oils on <i>Helicobacter pylori</i>

Elkousy, Rawah H.; Mostafa, Nada M.; Abd-Alkhalek, Ahmed M; Hassab, Mahmoud A. El; Al-Rashood, Sara T.; Eldehna, Wagdy M.; Eldahshan, Omayma A.

doi:10.1080/14756366.2022.2081846

Cited by 9 publications

(8 citation statements)

References 50 publications

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“…The combined oil sample showed the highest inhibitory effect against H. pylori. Clarithromycin demonstrated the same MIC value as the combined oil, at the same concentration used [31].…”

Section: Discussionmentioning

confidence: 66%

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Composition and Anti-Helicobacter pylori Properties of Essential Oils Obtained from Selected Mentha Cultivars

et al. 2023

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Helicobacter pylori infections are highly common amongst the global population. Such infections have been shown to be the cause of gastric ulcers and stomach carcinoma and, unfortunately, most cases are asymptomatic. Standard treatment requires antibiotics such as metronidazole or azithromycin to which many strains are now resistant. Mentha species have been used as a natural treatment for gastrointestinal diseases throughout history and essential oils (EOs) derived from these plants show promising results as potential antimicrobial agents. In this study, EOs obtained from the leaves and flowers of five cultivars of Mentha × piperita and M. spicata were examined by GC-MS. The investigated mints are representatives of four chemotypes: the menthol chemotype (M. × piperita ‘Multimentha’ and M. × piperita ‘Swiss’), the piperitenone oxide chemotype (M. × piperita ‘Almira’), the linalool chemotype (M. × piperita ‘Granada’), and the carvone chemotype (M. spicata ‘Moroccan’). The chemical composition of EOs from mint flowers and leaves was comparable with the exception of the Swiss cultivar. Menthol was the most abundant component in the leaves while menthone was highest in flowers. The H. pylori ATCC 43504 reference strain and 10 other H. pylori clinical strains were examined for their sensitivity to the EOs in addition to their major monoterpenoid components (menthol, menthone, carvone, dihydrocarvone, linalool, 1,8-cineole, and limonene). All tested mint EOs showed inhibitory activity against both the reference H. pylori ATCC 43504 strain (MIC 15.6–31.3 mg/L) and clinical H. pylori strains (MIC50/90 31.3–250 mg/L/62.5–500 mg/L). Among the reference monoterpenes, menthol (MIC50/90 7.8/31.3 mg/L) and carvone (MIC50/90 31.3/62.5 mg/L) had the highest anti-H. pylori activity, which also correlated with a higher activity of EOs containing these compounds (M. × piperita ‘Swiss’ and M. spicata ‘Moroccan’). A synergistic and additive interaction between the most active EOs/compounds and antibiotics possibly points to a new plant-based anti-H. pylori treatment.

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“…The combined oil sample showed the highest inhibitory effect against H. pylori. Clarithromycin demonstrated the same MIC value as the combined oil, at the same concentration used [31].…”

Section: Discussionmentioning

confidence: 66%

“…The results showed additive or synergistic effects. The synergistic effect of marjoram and petitgrain mandarin oils on anti-H. pylori activity was observed by Elkousy and colleagues [31]. The combined oil sample showed the highest inhibitory effect against H. pylori.…”

Section: Discussionmentioning

confidence: 78%

Composition and Anti-Helicobacter pylori Properties of Essential Oils Obtained from Selected Mentha Cultivars

et al. 2023

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“…The phytochemical approach for inhibiting the urease enzyme was used by Tawalbeh et al (2022) and Quy et al (2022) in their studies, while Elkousy et al (2022) employed the same approach to inhibit both urease and CagA, as outlined below. Elkousy et al (2022) examined the effectiveness of mandarin leaves and marjoram herb essential oils against H. pylori in comparison to clarithromycin using an in vitro method, and discovered that the combined effects of the oils were similar to those of clarithromycin.…”

Section: The In Silico Approaches Takenmentioning

confidence: 99%

IN SILICO INHIBITION OF UREASE AND CagA ENZYMES AS TARGETS AGAINST HELICOBACTER PYLORI: AN OVERVIEW ON CAPES 2022 DATABASE

Wanzeler,

Santos

2024

Arq. Ciênc. Saúde Unipar

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The proliferation, adaptive capability, and action of the Helicobacter pylori bacterium in the human stomach make it a worldwide public health issue. This bacterium is associated with gastrointestinal diseases such as stomach cancer. Adding to this challenge is the emergence of multi-drug-resistant and pan-drug-resistant pathogens among gram-negative bacteria, a category to which Helicobacter pylori belongs, making the elimination of this microorganism a daunting task. Therefore, there is an urgent need for the development of new treatment strategies to address this issue, and one potential way is the in silico method, specifically Computer-Aided Drug Design (CADD), which suggests new starting points in the search for innovative therapies and can be combined with traditional research methods (in vitro, in vivo, and ex-vivo). In this context, this review, utilizing data from the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) database, provides an overview of the various proposals discussed in 2022 that focus on the inhibition of proteins favorable to the colonization, virulence, and pathogenicity of H. pylori using in silico methods. Thus, several articles were inspected in the CAPES database. With a focus on molecular docking and/or molecular dynamics, pharmacokinetics, drug-like characteristics, and toxicity, 15 articles were identified that indicated the most studied enzymes in 2022 were urease and CagA. Furthermore, it was observed that the nature of the compounds used in inhibition was diverse - essential oils, flavonoids, pyrazolines, benzimidazoles, tetrahydropyrimidines, and benzothiazoles. All of these compounds were found to have the potential to be anti-urease and/or anti-CagA agents.

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“…including Salvia sclarea L., Origanum majorana, Mentha citrata Ehrh., Myrtus communis L. and Citrus aurantium L. (Elkousy et al, 2022). The chemical is usually one of the main components found within the essential oils of lavender.…”

Section: Acute Diuretic Activitymentioning

confidence: 99%

Evaluation and estimation of diuretic activity of the linalyl acetate in the rats

Rafique,

Mushtaq,

Ahmed

et al. 2023

Braz. J. Biol.

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This study aimed to explore the diuretic activity of linalyl acetate (LA). LA is an essential oil, it is an integral phyto-constituent of various plants. In this study, acute and chronic diuretic activities were explored by measuring the levels of different electrolytes and pH in the urine of experimental rats. Rats were divided into five groups. The control group was given 10 mg/kg normal saline, the treated group was given 10 mg/kg furosemide, and the remaining 3 groups received different doses of LA including 25, 50, and 75 mg/kg through intraperitoneal route, to determine its diuretic potential. Urine volume for acute diuretic activity was measured for 6 hours however for chronic diuretic activity was measured for 6 days. For a comparative study of LA with a control group and treated group with reference drug, diuretic index was used. Moreover, the underlying mechanism of the diuretic activity was also explored by comparing atropine, L-NAME, and indomethacin. The results of each group with 6 rats in each group were obtained by ± standard error of the mean of every group. Analysis of Variance (ANOVA) was used for statistical analysis. Results revealed that the LA 75 mg/kg dose showed comparable results as of furosemide. Moreover, this study revealed the involvement of muscarinic receptors to produce diuresis in comparison with atropine with very little involvement of prostanoids and no effect on NO pathway induced by indomethacin and L-NAME respectively. It is concluded that LA possess anti-diuretic potential. Muscarinic receptors might be involved in producing diuretic effects.

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GC/MS analysis and potential synergistic effect of mandarin and marjoram oils on Helicobacter pylori

Cited by 9 publications

References 50 publications

Composition and Anti-Helicobacter pylori Properties of Essential Oils Obtained from Selected Mentha Cultivars

Composition and Anti-Helicobacter pylori Properties of Essential Oils Obtained from Selected Mentha Cultivars

IN SILICO INHIBITION OF UREASE AND CagA ENZYMES AS TARGETS AGAINST HELICOBACTER PYLORI: AN OVERVIEW ON CAPES 2022 DATABASE

Evaluation and estimation of diuretic activity of the linalyl acetate in the rats

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