GC–MS Analysis of Bio-active Molecules Derived from Paracoccus pantotrophus FMR19 and the Antimicrobial Activity Against Bacterial Pathogens and MDROs

Abstract

The present investigation is focused on the study of chemical composition of a bioactive compound derived from a rumen isolate Paracoccus pantotrophus FMR19 using GC-MS and to find out the antibacterial activity of the extracted crude bioactive compounds against multidrug resistant organisms (MDROs) and other clinical pathogens. GC-MS analysis revealed that P. pantotrophus FMR19 produced eight major compounds that have been reported to exhibit antimicrobial property. The main components identified from hexane fraction are long chain alkanes, fatty alcohols, fatty acid methyl ester and aromatic hydrocarbons. These molecules are not only active against clinical pathogens such as Salmonella sp. and Proteus sp. and also effective against MDROs such as Metallo b lactamase and Pan drug resistant bacterial strains and Methicillin resistant Staphylococcus aureus.

Antibacterial activity of Centaurea pumilio L. root and aerial part extracts against some multidrug resistant bacteria

“…Pentadecane, tetradecane, hexadecane, nonadecane, heneicosane, eicosane, and heptacosane have previously been detected in C. napifolia, C. iconiensis, C. antiochia, C. aladaghensis, C. lanigera, C. iconiensis, and C. solstitialis, respectively [15,21,27]. The anticandidal activity of the C. pumilio chloroform aerial part extract might be accredited to 3oxo-10(14)-epoxyguai-11(13)-en-6,12-olide and curan-17-oic acid,2,16-didehydro-20-hydroxy-19-oxo,methylester which have been described as antifungal agents [41,42]. In addition, Cis-13-eicosenoic acid was detected in Camilla sinesis extract that was described for its antimicrobial activity against MRSA and MDR P. aeureginosa with MIC of 400 μg/mL and 800 μg/mL, respectively [43].…”

“…The GC-MS analysis of the C. pumilio chloroform aerial part extract reported hydrocarbons as the most abundant constituents and showed antibacterial activity against the clinical and MDR pathogens [37][38][39][40][41]. Pentadecane and heptadecane are the major hydrocarbons and these have been reported for their antimicrobial activity [39][40][41]. Tetradecane, hexadecane, nonadecane, and heneicosane were recorded as antimicrobial agents [41].…”

“…Pentadecane and heptadecane are the major hydrocarbons and these have been reported for their antimicrobial activity [39][40][41]. Tetradecane, hexadecane, nonadecane, and heneicosane were recorded as antimicrobial agents [41]. Eicosane, a long-chain fatty acid, has been reported for its antibacterial activity [41].…”

“…Tetradecane, hexadecane, nonadecane, and heneicosane were recorded as antimicrobial agents [41]. Eicosane, a long-chain fatty acid, has been reported for its antibacterial activity [41]. Pentadecane, tetradecane, hexadecane, nonadecane, heneicosane, eicosane, and heptacosane have previously been detected in C. napifolia, C. iconiensis, C. antiochia, C. aladaghensis, C. lanigera, C. iconiensis, and C. solstitialis, respectively [15,21,27].…”

Antimicrobial potentiality of actinobacteria isolated from two microbiologically unexplored forest ecosystems of Northeast India

“…no. Retention time (min) Compound Molecular weight (MW) Area % Reported activity References 1 20.098 2,5-bis(1,1-dimethylethyl) Phenol 206 21.11 Antifungal, Antioxidant, Antimicrobial [ 64 , 65 , 70 ] 2 16.433 Benzeneacetic acid 136 10.22 Antifungal, Antimicrobial [ 66 , 67 ] 3 24.217 1-Nonadecene 266 8.95 Antifungal, Antimicrobial [ 69 ] 4 28.123 Dibutyl phthalate 278 6.61 Antimicrobial [ 71 ] 5 26.933 7,9-Di-tert-butyl-1-oxaspiro(4,5)deca-6,9-diene-2,8-dione 276 3.95 No reported antimicrobial This study 6 35.18 1-Heptacosanol 396 3.39 Antimicrobial, Antioxidant [ 72 ] 7 25.631 Phthalic acid, hex-3-yl isobutyl ester 320 2.72 No reported antimicrobial This study 8 24.725 Isopropyl myristate 270 2.37 Antimicrobial, Antioxidant [ 73 ] 9 40.539 Heptacosyl heptafluorobutyrate 592 1.51 No reported antimicrobial This study 10 39.928 Phthalic acid, di(2-propylpentyl) ester …”