Gc-MS Analysis of Bioactive Compounds in Ethanol Leaves Extract of Sphenocentrum Jollyanum and Their Biological Activities.

Abstract

Sphenocentrum jollyanum is a plant genus of the family Menispermaceae. It has high medicinal importance as it is used traditionally to treat various diseases such as jaundice, breast engorgement related to the menstrual cycle, tumour, fibroids and improve the health of people. The present investigation was carried out to analyze the bioactive compounds present in ethanol crude extract of Sphenocentrum jollyanum leaves using GC-MS analysis. GC-MS analysis of ethanol extract Sphenocentrum jollyanum was done using a 7890A GC system (Agilent Technologies), coupled with 5977B MSD (Agilent Technologies) while the mass spectra of the compounds found in the extract was matched with the National Institute of Standards and Technology (NIST) library. A total of 45 bioactive compounds representing 99.98% of the total extract based on the retention time, peak area, molecular formula, molecular weight, and biological activities were identified by GC-MS which ranges from high molecular weight to low molecular weight compounds. The major compounds identified with their peak area percentages were 2,4-Di-tertbutylphenol, (21.05%), Z-8-Methyl-9-tetradecenoic acid (19.12), Hexadecanoic acid, ethyl ester (7.86%), Diisooctyl phthalate (7.13%), Phytol, Oleic Acid (7.03), 6,9,12- Octadecatrien-1-ol (6.65%), 3-Eicosene, (E)-(4.63%), 2-Methyl-Z, Z-3,13-octadecadienol (4.24%), n- Hexadecanoic acid (4.09%), trans-13-Octadecenoic acid (3.81%), Cyclohexene, 4-(4-ethylcyclohexyl) -1- pentyl- (3.74%), Dibutyl phthalate (3.20%), and 9-Oxabicyclo (6.1.0) nonane, cis-(3.18%). The presence of these major phytoconstituents in the leaf extract provides various biological activities including antifungal, antibacterial, antioxidant, anti-inflammatory, and anti-tumour which supports the ethno-medicinal uses of the plant in curing diseases. We recommend

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