GC-MS analysis of phytochemical compounds of Opuntia megarrhiza (Cactaceae), an endangered plant of Mexico

Abstract

Opuntia megarrhiza is an endemic plant used in Mexican traditional medicine for the treatment of bones fractures in humans and domestic animals. One of the most used technique for the detection and characterization of the structure of phytochemical compounds is the Gas Chromatography Coupled to Mass Spectrometry. The goals of the present study were to identify and characterize the phytochemical compounds present in wild individuals of O. megarrhiza using this analysis. We used chloroform and methanol extracts from cladodes, and they were analyzed by gas chromatography-electron impact-mass spectrometry. We obtained 53 phytochemical compounds, 19 have been previously identified with some biological activity. Most of these compounds are alkanes, alkenes, aromatic hydrocarbons, fatty acids, and ketones. We detected some fragmentation patterns that are described for the first time for this species. The variety of metabolites presents in O. megarrhiza justifies the medicinal use of this plant in traditional medicine and highlight it as a source of phytochemical compounds with potential in medicine and biotechnology.

Identification, validation and quantification of thymoquinone in conjunction with assessment of bioactive possessions and GC-MS profiling of pharmaceutically valuable crop Nigella (Nigella sativa L.) varieties

“…The chemical composition of the seed oil isolated from Nigella sativa L., varieties utilized in the study was determined through GC-MS analysis following the AOCS Method CE 1-62 and Cupido et al (2022) with minor modifications. The GC-MS profiling was done using Agilent (GC-7820 A, MS-5975; Agilent Technologies, Santa Clara, CA, USA) equipped with an HP 5 (universal column; 30 m × 0.325 mm, film thickness 0.25 μm) Agilent J&W GC column with an autosampler.…”

“…Considering that it is a very accurate method for characterising and identifying metabolites. The GC-MS is a useful tool for metabolite profiling because it is vital for the qualitative and quantitative characterization of chemical compound (metabolite) present in plant cells ( Cupido et al, 2022 ). Genetic variability is an important factor for varying extents of metabolite obtained in different varieties of crop plants.…”

Aproximaciones evolutivas en etnobotánica de plantas medicinales y bioprospección

“…Tal es el caso de Opuntia megarrhiza Rose (Cactaceae) (Figura 2), una especie endémica del desierto Chihuahuense en México, la cual se utiliza como medicina tradicional para el tratamiento de fracturas de huesos tantos en animales de granja como en seres humanos. Recientemente se detectaron 19 com-puestos bioactivos volátiles por medio de la GC-MS (Cromatografía de gases acoplada a espectrometría de masas) en cladodios de O. megarrhiza por lo que esta especie y sus parientes cercanos podría representar una alternativa para encontrar compuestos fitoquímicos con potencial para la medicina y biotecnología (Cupido et al 2022). Casos como este resaltan la relevancia de estudiar otras especies del género Opuntia, cuya diversificación ocurrió en México hace al menos 4.9 millones de años atrás, generando cientos de especies (Hernández-Hernández et al 2014), lo que exhorta aplicar nuevas técnicas filogenéticas que permitan el descubrimiento de nuevas drogas en linajes diversos y de distribución restringida.…”

“…Compuestos fitoquímicos predominantes en extractos de cladodios en Opuntia megarrhiza, especie endémica del desierto Chihuahuense, utilizada como medicina tradicional en el tratamiento de fracturas de huesos tanto de seres humanos como animales. Modificado deCupido et al 2022.…”

Phytochemical profiling of ethanolic extract of bee pollen using Gas Chromatography Mass Spectroscopy and its in silico analysis of hypocholeterolemic activity

“…These compounds were reported for their therapeutic efficiency. Hexadecanoic acid (16.5%) (saturated fatty acids) has potential anti-inflammatory, antiallopactic and antioxidant properties (Cupido et al, 2022).…”