GC-MS analysis of volatile oil and fatty acids composition of Abutilon hirtum (Lam.) Sweet leaves

Gomaa, Alshymaa Abdel-Rahman; Desoukey, Samar Yehia; Kamel, Mohamed S.

doi:10.21608/jabps.2021.57135.1116

Cited by 3 publications

(2 citation statements)

References 3 publications

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“…A previous study [ 51 ] suggested that a decrease in MI to 20% induces sublethal effects on the test organisms, but a reduction in concentration to 50% causes fatal effects on the test organisms [ 52 ]. The inhibition of the division rate may be revealed by the phytochemical content of A. hirtum , such as phenolic acids, flavonoids, alkaloids, triterpenes, coumarins, sterols, iridoids, and essential volatile oils [ 19 , 53 ]. An interesting suggestion is that methoxyacetic acid (a part of one of these components) interferes with the metabolic pathways in the cell, resulting in cellular damage and cell abnormalities during cell division [ 54 ].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the antioxidant activity, genotoxic, and cytotoxic effects of the ethanolic leaves extract of Abutilon hirtum (Lam.) Sweet using in vitro assays

Qari¹

2023

Heliyon

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Section: Discussionmentioning

confidence: 99%

Evaluation of the antioxidant activity, genotoxic, and cytotoxic effects of the ethanolic leaves extract of Abutilon hirtum (Lam.) Sweet using in vitro assays

Qari¹

2023

Heliyon

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“…Fatty acid composition analysis was conducted using GC-MS on wild type and mutant 1. The identification of fatty acids relied on retention time and mass spectrum characteristics with the method proposed by Gomaa et al (2021). From the analysis, various fatty acids were successfully detected, and a comparison between the wild-type and the mutant can be made based on the total detected fatty acids.…”

Section: Lipid Composition In Wild Type and Mutantsmentioning

confidence: 99%

Random Mutagenesis of Lipomyces maratuensis InaCC Y720 Using Commercial UV Lamp To Increase Lipid Production

Lestari,

Kanti,

Ilmi

2024

Al-Kauniyah J. Biol.

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Oleaginous yeasts are capable of accumulating high lipid concentration up to 20% of dry cell weight. High lipid content, a shorter life cycle, and similar a fatty acid composition to vegetable oils makes oleaginous yeast a potential lipid producer. Lipomyces maratuensis InaCC Y720 is a novel species isolated from Maratua Island, East Kalimantan, which has been reported as a potential yeast lipid producer. However, lipid productivity of the yeast is needed to be increased to make it suitable for an industrial scale. The aim of this study is to obtain potential mutant strains for the biodiesel industry. Random mutagenesis was applied by using commercial UV-C lamp on the strain which resulting in an 80% death rate after three hours irradiation. Subsequent treatment was carried out using cerulenin as a selection agent for mutans, yielding six mutant strains. Among these strains, mutant 1 produced the highest lipid production, with a lipid concentration of 0.072 g/L and a lipid percentage of 8.603%. Nevertheless, when compared to the wild type, the lipid productivity of mutant 1 is low. Based on these results, the mutagenesis approach using commercial lamp UV-C has not obtained the expected mutants, so it is recommended to use different methods for future study.AbstrakKhamir oleaginous memiliki kemampuan dapat mengakumulasi lipid hingga 20% dari berat kering selnya. Tingginya kadar lipid yang diproduksi, siklus hidup yang pendek serta komposisi lipid yang mirip dengan minyak tumbuhan dapat menjadikan khamir sebagai alternatif penghasil lipid. Lipomyces maratuensis InaCC Y720 merupakan spesies baru yang diisolasi dari Pulau Maratua, Kalimantan Timur yang dilaporkan sebagai khamir penghasil lipid potensial. Namun, produktivitas lipid khamir tersebut perlu ditingkatkan agar sesuai untuk skala industri. Tujuan dalam studi ini adalah mendapatkan strain mutan yang potensial untuk industri biodiesel. Metode mutagenesis secara acak dilakukan dengan menggunakan lampu UV-C komersial pada strain yang menghasilkan tingkat kematian 80% selama tiga jam penyinaran. Setelah itu, dilakukan perlakuan lebih lanjut dengan penggunaan serulenin sebagai agen seleksi mutan. Proses seleksi menghasilkan enam strain mutan. Di antara keenam strain mutan, mutan 1 menghasilkan jumlah lipid tertinggi dengan berat lipid 0,072 g/L dengan persentase lipid yaitu 8,603%. Namun, dibandingkan dengan wild type, produktivitas lipid mutan 1 lebih rendah. Berdasarkan hasil ini, mutagenesis menggunakan lampu UV-C komersial belum mendapatkan mutan yang diharapkan sehingga disarankan penggunaan metode yang berbeda untuk penelitian selanjutnya.

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GC/MS chemical profiling and isolation of secondary metabolites from Leontice ewersmannii

Omirzakova,

Samy,

Kiyekbayeva

et al. 2024

Natural Product Research

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GC/MS chemical profiling of the aerial parts of Leontice ewersmanni revealed the presence of 20 compounds in n-hexane fraction and 3 compounds in basic DCM fraction. Among the identified compounds alkaloids, fatty acids and their esters, fatty alcohols, hydrocarbons and sterol were detected. Phytochemical investigation of the aerial parts of L. ewersmanni led to isolation of 17 compounds including two 1-heptacosanol (1), phytol (2), lupenone (3), palmitic acid (4), oleic acid (5), stigmasterol (6), stigmast-7-en-3-ol (7), methyl palmitate (8), methyl linoleate (9), butyl palmitate (10), butyl linoleate (11), dimethyl dodecanedioate (12), methyl cerebronate (13), β-sitosterol ( 14), β-sitosterol 3-O-β-glucopyranoside (15), sophoridine (16) and camoensine (17). The structures of the isolated compounds were determined by 1D NMR, HR-ESI-MS, and GC-MS. Additionally, the antimicrobial activity of the total extract and different fractions was screened, and they exhibited no inhibition of the growth at the tested concentration ranging from 8 to 200 μg/mL.

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GC-MS analysis of volatile oil and fatty acids composition of Abutilon hirtum (Lam.) Sweet leaves

Cited by 3 publications

References 3 publications

Evaluation of the antioxidant activity, genotoxic, and cytotoxic effects of the ethanolic leaves extract of Abutilon hirtum (Lam.) Sweet using in vitro assays

Evaluation of the antioxidant activity, genotoxic, and cytotoxic effects of the ethanolic leaves extract of Abutilon hirtum (Lam.) Sweet using in vitro assays

Random Mutagenesis of Lipomyces maratuensis InaCC Y720 Using Commercial UV Lamp To Increase Lipid Production

GC/MS chemical profiling and isolation of secondary metabolites from Leontice ewersmannii

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