GC-MS assay for acetaminophen in human hair segments

Saito, Takeshi; Morita, Seiji; Inoue, Shigeaki; Yamamoto, Ikuo; Inokuchi, Sadaki

doi:10.1007/s11419-008-0044-z

Cited by 9 publications

(8 citation statements)

References 12 publications

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“…Methylphenidate was detected only in the proximal segment at 1.0 pg/mg, while the result was negative in the other two segments. These results support 1 3 ment to these analyses to increase the window of detection, and segmentation of the hair permits differentiation of a single exposure from chronic use of the drug [5][6][7][8]. Moreover, due to the long delays that are frequently encountered between exposure and reporting to the police, hair is often the only matrix capable of providing corroborative evidence of a crime.…”

Section: Introductionsupporting

confidence: 57%

Violence under the influence of methylphenidate as determined by hair analysis

Kintz¹,

Villain²

2010

Forensic Toxicol

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Section: Introductionsupporting

confidence: 57%

Violence under the influence of methylphenidate as determined by hair analysis

Kintz¹,

Villain²

2010

Forensic Toxicol

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“…Penelitian Saito (2008) telah berhasil melakukan analisis acetaminophen menggunakan spesimen rambut pada kasus keracunan dalam kondisi overdose (OD). Dari penelitian ini diketahui pula bahwa limit deteksi GC-MS adalah 0,1 ng/mg dengan jumlah spesimen rambut yang digunakan sebanyak 20 mg.…”

Section: Parasetamolunclassified

“…Sebanyak 200 mg spesimen rambut ditimbang. Kemudian didekontaminasi dengan 5 mL diklorometana selama 2 menit pada suhu ruang, 5 mL air hangat selama 2 menit dan 5 mL diklorometana selama 2 menit (Saito, 2008). Setelah didekontaminasi, spesimen rambut digunting menjadi kecil dan diinkubasi pada 45 0 C selama 2 jam dalam 1 mL metanol.…”

Section: Preparasi Dan Analisis Spesimen Rambutunclassified

Pengembangan Metode GC-MS untuk Penetapan Kadar Acetaminophen pada Spesimen Rambut Manusia

Darmapatni

2016

JBP

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ABSTRAKRambut dapat digunakan sebagai pilihan dalam melakukan analisis senyawa obat di dalam tubuh khususnya pada kasus-kasus penyalahgunaan obat atau keracunan yang bersifat kronik. Telah dilakukan penelitian yang bertujuan untuk mengembangan metode untuk penetapan kadar acetaminophen pada spesimen rambut manusia menggunakan instrumen GC-MS. Sampel rambut diambil pada panjang 0-3 cm, 0-6 cm dan 0-10 cm pada 10 orang pasien yang mendapatkan terapi parasetamol. Preparasi sampel terdiri dari tahap dekontaminasi, destruksi, ekstraksi dan derivatisasi pada masing-masing spesimen rambut. Kemudian ekstrak diinjeksikan pada sistem GC-MS. Hasil penelitian menunjukkan bahwa acetaminophen dapat terdeteksi pada spesimen rambut dalam bentuk acetaminophen-TMS akibat adanya perlakuan derivatisasi menggunakan BSTFA yang mengandung TMCS 1%. Pada panjang spesimen rambut 0-3 cm diperoleh konsentrasi 0,1761-0,3392 ng/mg spesimen rambut, pada panjang spesimen rambut 0-6 cm diperoleh hasil 0,2081-0.4845 ng/mg spesimen rambut dan pada panjang spesimen rambut 0-10 cm konsentrasi acetaminophen-TMS dalam sampel adalah 0,2473-0,5782 ng/mg spesimen rambut. Kata kunci : acetaminophen, rambut, Gas Chromatography - Mass Spectrometry

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“…Due to the possible side effects for humans as well as the negative impact on the environment, it is extremely important to develop sensitive and accurate methods for the determination of the presented drugs in samples of biological fluids and environmental samples. There are many analytical methods such as high-performance liquid chromatography (HPLC) [7][8][9], liquid chromatography-tandem mass spectrometry (LC-MS/MS) [10][11][12], gas chromatography-mass spectrometry (GC-MS) [13][14][15], and spectrophotometry [16][17][18], which are used to determine paracetamol, diclofenac, and tramadol. However, these methods are generally costly, requiring a time-consuming sample preparation step.…”

Section: Introductionmentioning

confidence: 99%

Electrochemically Activated Screen-Printed Carbon Sensor Modified with Anionic Surfactant (aSPCE/SDS) for Simultaneous Determination of Paracetamol, Diclofenac and Tramadol

et al. 2021

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In this work, an electrochemically activated screen-printed carbon electrode modified with sodium dodecyl sulfate (aSPCE/SDS) was proposed for the simultaneous determination of paracetamol (PA), diclofenac (DF), and tramadol (TR). Changes of surface morphology and electrochemical behaviour of the electrode after the electrochemical activation with H2O2 and SDS surface modification were studied by scanning electron microscopy (SEM), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). The influence of various parameters on the responses of the aSPCE/SDS such as pH and concentration of the buffer, SDS concentration, and techniques parameters were investigated. Using optimised conditions (Eacc. of −0.4 V, tacc. of 120 s, ΔEA of 150 mV, ν of 250 mV s−1, and tm of 10 ms), the aSPCE/SDS showed a good linear response in the concentration ranges of 5.0 × 10−8–2.0 × 10−5 for PA, 1.0 × 10−9–2.0 × 10−7 for DF, and 1.0 × 10−8–2.0 × 10−7 and 2.0 × 10−7–2.0 × 10−6 mol L−1 for TR. The limits of detection obtained during the simultaneous determination of PA, DF, and TR are 1.49 × 10−8 mol L−1, 2.10 × 10−10 mol L−1, and 1.71 × 10−9 mol L−1, respectively. The selectivity of the aSPCE/SDS was evaluated by examination of the impact of some inorganic and organic substances that are commonly present in environmental and biological samples on the responses of PA, DF, and TR. Finally, the differential pulse adsorptive stripping voltammetric (DPAdSV) procedure using the aSPCE/SDS was successfully applied for the determination of PA, DF, and TR in river water and serum samples as well as pharmaceuticals.

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GC-MS assay for acetaminophen in human hair segments

Cited by 9 publications

References 12 publications

Violence under the influence of methylphenidate as determined by hair analysis

Violence under the influence of methylphenidate as determined by hair analysis

Pengembangan Metode GC-MS untuk Penetapan Kadar Acetaminophen pada Spesimen Rambut Manusia

Electrochemically Activated Screen-Printed Carbon Sensor Modified with Anionic Surfactant (aSPCE/SDS) for Simultaneous Determination of Paracetamol, Diclofenac and Tramadol

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