GC-MS based targeted metabolomics approach for studying the variations of phenolic metabolites in artificially ripened banana fruits

Tallapally, Maheshwari; Sadiq, Asif Shaikh; Mehtab, Vazida; Chilakala, Shireesha; Vemula, Madhu; Sumana, C.; Upadhyayula, Vijayasarathi

doi:10.1016/j.lwt.2020.109622

Cited by 20 publications

(4 citation statements)

References 22 publications

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“…Sinapic acid has been identi ed through LCMS/MS analysis in banana roots (Sebatian Fenolik dan Komposisinya dalam Tindak Balas Pertahanan Pokok Pisang Berangan yang et al 2021). Furthermore, it has been observed that sinapic acid levels increase in bananas treated with arti cial ripeners (Tallapally et al 2020). The LARESI method revealed pyruvic acid to be the compound with the lowest molecular weight in this study.…”

Section: Resultsmentioning

confidence: 99%

Ambient mass spectrometry imaging of banana (Musa spp.) tissue with infrared laser-based selected reaction monitoring mass spectrometry imaging

Nizioł

Misiorek

Ruman

2023

Preprint

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In this study, for the first time we present the application of an infrared (IR) laser ablation-remote-electrospray ionization (LARESI) platform coupled to a tandem mass spectrometer (MS/MS) operated in selected reaction monitoring (SRM) or multiple reaction monitoring (MRM) modes for targeted metabolite imaging in intact plant tissues. The primary objective of this study was to investigate the spatial distribution of specific metabolites in banana tissue sections from two commercially available banana varieties: Red Dacca and Cavendish banana (Musa acuminata from AAA banana cultivar group). To support MSI results, an extensive analysis of banana tissue extracts was conducted using ultra-high-performance liquid chromatography and ultra-high-resolution mass spectrometry (UHPLC-UHRMS). In the Cavendish banana, 12 metabolites were successfully identified, while Red Dacca Bananas were found to contain 16 amino acids. The spatial distribution of some of these compounds found in bananas was presented for the first time. The results highlight the potential of the LARESI MSI technique as a highly sensitive and rapid method for targeted imaging of plant tissues. Importantly, this approach eliminates the need for high vacuum conditions and the pretreatment of biological materials, making it an efficient and promising tool for studying metabolites in plant tissues.

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Section: Resultsmentioning

confidence: 99%

Ambient mass spectrometry imaging of banana (Musa spp.) tissue with infrared laser-based selected reaction monitoring mass spectrometry imaging

Nizioł

Misiorek

Ruman

2023

Preprint

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“…Sinapic acid has been identified through LC MS/MS analysis in banana roots (Shi Ming et al 2021). Furthermore, it has been observed that sinapic acid levels increase in bananas treated with artificial ripeners (Tallapally et al 2020). Previous research on extracts from different morphological parts of bananas indicated that ferulic acid is more abundant in leaf sheaths and floral stalks, whereas rachis contains it in smaller quantities (Oliveira et al 2006).…”

Section: Resultsmentioning

confidence: 99%

Infrared Laser-Based Selected Reaction Monitoring Mass Spectrometry Imaging of Banana (Musa spp.) Tissue—New Method for Detection and Spatial Localization of Metabolites in Food

Nizioł,

Misiorek,

Krupa

et al. 2023

Food Anal. Methods

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In this study, for the first time, we present the application of an infrared (IR) laser ablation-remote-electrospray ionization (LARESI) platform coupled to a tandem mass spectrometer (MS/MS) operated in selected reaction monitoring (SRM) mode for targeted metabolite imaging in intact plant tissues. We examined the distribution of specific metabolites in two banana varieties: Red Dacca and Cavendish. To support MSI results, an extensive analysis of banana tissue extracts was conducted using ultra-high-performance liquid chromatography and ultra-high-resolution mass spectrometry (UHPLC-UHRMS). In the Cavendish banana, 12 metabolites were successfully identified, while Red Dacca bananas were found to contain 16 amino acids. The spatial distribution of some of these compounds found in bananas was presented for the first time. This approach eliminates the need for high vacuum conditions and the pretreatment of biological materials, making it an efficient and promising tool for studying metabolites in plant tissues.

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“…Metabolomics has been around for a while, but it hasn't been applied to many important food groups, including fruits. The analysis of volatiles is useful for a variety of tasks in the quality control of fruit and nutraceuticals, such as genotype or geographic origin differentiation and maturity stage differentiation (Khalil et al ., 2017; Tallapally et al ., 2020). GC in tandem with high‐throughput MS enabled the identification of volatiles, particularly in fruit flavour compounds to better understand their complex interactions (Yao et al ., 2018; Colantonio et al ., 2022).…”

Section: Metabolomics Techniques For Exploring the Complexity Of Frui...mentioning

confidence: 99%

Recent progress in metabolomics research: insights into fruit metabolome, harvesting techniques and health‐promoting bioactives

Dadwal,

Aroor,

Joshi

et al. 2024

Int J of Food Sci Tech

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SummaryThe use of cutting‐edge omics technology to edible fruits has transformed the disciplines of fruit biology, pre‐ and post‐harvest investigations, metabolite biosynthesis and the identification of novel therapeutic fruit bioactives for health by leveraging varied omics data. Combining modern analytical chromatography tools (LC, GC) with mass spectrometry has significantly improved our ability to examine complex fruit tissues or extracted components, advancing our understanding of the fruit metabolome. Studies aiming at understanding the full metabolome and future quality characteristics have concentrated on quantifying the number of metabolites in edible fruit species and cultivars from diverse geographical locations. These studies have also helped to develop new databases for precise and comprehensive qualitative analysis of metabolites, allowing for the analysis of metabolite biosynthesis pathways to identify differences in metabolites among developed hybrids, metabolite origins and potential derivatives. Bioactive metabolite information is currently being utilised to manage illnesses, provide nutrition and creation of novel food products. Furthermore, this research has helped us better understand fruit quality and how metabolites interact with biological systems. In conclusion, this review emphasises the importance of metabolomics approaches in studying fruit metabolomes in the context of current research perspectives.

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GC-MS based targeted metabolomics approach for studying the variations of phenolic metabolites in artificially ripened banana fruits

Cited by 20 publications

References 22 publications

Ambient mass spectrometry imaging of banana (Musa spp.) tissue with infrared laser-based selected reaction monitoring mass spectrometry imaging

Ambient mass spectrometry imaging of banana (Musa spp.) tissue with infrared laser-based selected reaction monitoring mass spectrometry imaging

Infrared Laser-Based Selected Reaction Monitoring Mass Spectrometry Imaging of Banana (Musa spp.) Tissue—New Method for Detection and Spatial Localization of Metabolites in Food

Recent progress in metabolomics research: insights into fruit metabolome, harvesting techniques and health‐promoting bioactives

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