GC-MS Chemical Characterization of Main Components of Smilax Domingensis Wild. in Cuba

Soledispa, Pilar A.; González, José; Cuéllar, Armando Cuéllar; Pérez, Julio; Monan, Max

doi:10.5296/jas.v6i2.13099

Cited by 3 publications

(1 citation statement)

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“…The main chemical constituents identified in KKRE comprised palmitic acid, as reported in prior studies investigating the ethanolic leaf extract of P. amarus [ 52 ] and the methanolic extract of the Smilax China plant within the Smilax genus [ 53 ]. Furthermore, margarinic acid was documented in the methanolic extract of Smilax zeylanica [ 54 ], while malic acid and oleic acid ethyl ester were also detected in the rhizome of the ethanolic Smilax domingensis extract [ 55 ]. The chemical constituents of KKRE exhibited α -glucosidase inhibitory activity; for instance, palmitic acid demonstrated potential inhibitory effects on the α -glucosidase enzyme [ 35 ], while malic acid also showed similar inhibitory potential [ 56 ].…”

Section: Discussionmentioning

confidence: 99%

Alpha-Glucosidase Inhibition, Antioxidant Activities, and Molecular Docking Study of Krom Luang Chumphon Khet Udomsak, a Thai Traditional Remedy

Limcharoen,

Chaniad,

Chonsut

et al. 2024

Advances in Pharmacological and Pharmaceutical Sciences

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Krom Luang Chumphon Khet Udomsak remedy (KKR) has traditionally been used as an alternative treatment, particularly for hyperglycemia; however, its therapeutic efficacy has not been scientifically validated. Thus, this study aims to investigate the potential inhibitory and antioxidant effects of α-glucosidase enzyme and characterize the chemical profile of KKR extracts using gas chromatography-mass spectrometry (GC-MS). The investigation highlights both KKR extracts as potent inhibitors of α-glucosidase, with the ethanolic extract of KKR (KKRE) displaying an IC50 value of 46.80 µg/mL and a noncompetitive mode of action. The combination of ethanolic and aqueous extracts of KKR (KKRE and KKRA, respectively) with acarbose exhibited a synergistic effect against the α-glucosidase. The KKRE extract displayed strong scavenging effects in the DPPH assay (IC50 156.3 µg/mL) and contained significant total phenolic (172.82 mg GAE/g extract) and flavonoid (77.41 mg QE/g extract) contents. The major component of KKRE is palmitic acid (15.67%). Molecular docking revealed that the major compounds interacted with key amino acid residues (ASP215, GLU277, HIS351, ASP352, and ARG442), which are crucial for inhibiting α-glucosidase. Notably, campesterin had a more significant influence on α-glucosidase than acarbose, with low binding energy. These findings underscore the significance of KKR in traditional medicine and suggest that it is promising treatment for diabetes mellitus. Further studies using animal model will provide valuable insights for advancing this research.

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Section: Discussionmentioning

confidence: 99%

Alpha-Glucosidase Inhibition, Antioxidant Activities, and Molecular Docking Study of Krom Luang Chumphon Khet Udomsak, a Thai Traditional Remedy

Limcharoen,

Chaniad,

Chonsut

et al. 2024

Advances in Pharmacological and Pharmaceutical Sciences

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Plastome comparison reveals hotspots of nucleotide diversity and positive selection pressure on accD, matK, psaA and rbcL genes in Smilacaceae

Sukhramani,

Maurya,

Choudhary

2024

Braz. J. Bot

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Challenge of Utilization Vegetal Extracts as Natural Plant Protection Products

et al. 2020

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Natural plant protection products (known as biopesticides), derived from natural materials (plants, bacterial strains, and certain minerals) that can be used to control pests, are an alternative to plant protection chemicals (known as pesticides) due to certain advantages: less toxic to humans and the environment, no release/leaching of harmful residues, and usually much specific to the target pests. This review focuses on the systematization of information highlighting the main advantages related to the natural plant protection products used, the extractive methods of obtaining them, their physical-chemical analysis methodology, the specific constituents responsible for their pesticide effects, the mechanisms of action, and methods for direct application on vegetable crops or on seeds stored in warehouses, in order to eliminate the adverse effects occurred in the case of plant protection chemicals use. Special attention has been accorded to natural plant protection products from the spontaneous flora of Moldova (Romania’s macroeconomic region NE), which can be considered a resource of valuable secondary metabolites, especially in the form of vegetable essential oils, with biological effects and biopesticide routes of action. All presented information concludes that biopesticides can successfully replace the chemical plant protection products on small farms and especially in silos (seeds and cereals).

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GC-MS Chemical Characterization of Main Components of Smilax Domingensis Wild. in Cuba

Cited by 3 publications

References 2 publications

Alpha-Glucosidase Inhibition, Antioxidant Activities, and Molecular Docking Study of Krom Luang Chumphon Khet Udomsak, a Thai Traditional Remedy

Alpha-Glucosidase Inhibition, Antioxidant Activities, and Molecular Docking Study of Krom Luang Chumphon Khet Udomsak, a Thai Traditional Remedy

Plastome comparison reveals hotspots of nucleotide diversity and positive selection pressure on accD, matK, psaA and rbcL genes in Smilacaceae

Challenge of Utilization Vegetal Extracts as Natural Plant Protection Products

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