GC-MS combined with AMDIS and Koáts retention index to investigate dynamic change rules of volatile components from Atractylodis Macrocephalae Rhizoma with different stir-baking degrees

Abstract

To investigate the dynamic change rules of volatile components from Atractylodis Macrocephalae Rhizoma with different stir-baking degrees (from slight stir-baking, stir-baking to yellow, stir-baking to brown, to stir-baking to scorch). In the present experiment, the Atractylodis Macrocephalae Rhizoma samples with different stir-baking degrees were collected at different processing time points. The contents of volatile oil in various samples were determined by steam distillation method, and the volatile compounds were extracted by using static headspace sampling method. Gas chromatography-mass spectrography (GC-MS) and automated mass spectral deconrolution and identification system (AMDIS) were combined with Kováts retention index to analyze the chemical constituents of the volatile compounds. The results showed that with the deepening of the stir-baking degree, the content of volatile oil was decreased step by step in 4 phases, and both the compositions and contents of volatile components from Atractylodis Macrocephalae Rhizoma showed significant changes. The results showed that the dynamic change rules of volatile components from Atractylodis Macrocephalae Rhizoma in the process of stir-baking were closely related to the processing degree; in addition, Atractylodis Macrocephalae Rhizoma and honey bran had adsorption on each other. These results can provide a scientific basis for elucidating the stir-baking (with bran) mechanism of Atractylodis Macrocephalae Rhizoma.

Application of digital-intelligence technology in the processing of Chinese materia medica

“…In the PCMM, heat refers to the size and duration of fire when the drug is heated, which is a key factor affecting the quality of decoction pieces. Studies have shown that with the change of fire degree, the content of volatile components, amino acids and reduced sugars in drugs have changed significantly ( Chen et al, 2016 ; Hu et al, 2016 ). Therefore, through the digital technology, the various influencing factors in the processing are objectively quantified, and then the artificial intelligence technology is used to adjust and control the fire, time, temperature and processing end point, which is conducive to the standardization of processing technology and the quality of decoction pieces.…”