GC–MS method for the simultaneous determination of β-blockers, flavonoids, isoflavones and their metabolites in human urine

Magiera, Sylwia; Uhlschmied, C.; Rainer, Matthias; Huck, Christian W.; Baranowska, Irena; Bonn, Guenther K.

doi:10.1016/j.jpba.2011.04.024

Cited by 60 publications

(40 citation statements)

References 40 publications

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“…Initially, in our studies, an ultra-performance liquid chromatography methods with UV or MS/MS detection and gas chromatography method with MS detection (GC-MS) were developed for determination of flavonoids and metabolites in human urine samples Magiera et al 2011;Magiera et al 2012). However, these methods were not suitable for the simultaneous determination of selected polyphenol compounds in L. barbarum L. fruits.…”

Section: Chromatographic Methods Developmentmentioning

confidence: 99%

Chromatographic Determination of Phenolic Acids and Flavonoids in Lycium barbarum L. and Evaluation of Antioxidant Activity

Magiera

Zareba

2015

Food Anal. Methods

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Polyphenols are the important active compounds present in the fruits of Lycium barbarum L. The objectives of this study were to develop an ultra-high performance liquid chromatography with UV detection (UHPLC-UV) method for qualitative and quantitative analyses of phenolic acids and flavonoids in fruits of L. barbarum L. The isolation and enrichment of polyphenols from fruits were carried out by ultrasound-assisted extraction (UAE) in conjunction with solid-phase extraction (SPE). The analytes were separated on a Poroshell 120 EC-C18 column using a binary mobile phase composed of aqueous 0.05 % trifluoroacetic acid and acetonitrile in the gradient elution mode. Under these conditions, phenolic acids and flavonoids were separated in 11 min. The selectivity of the developed UHPLC-UV method was confirmed by comparison with ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) analysis. The validation parameters such as linearity, sensitivity, precision, and accuracy were found to be highly satisfactory. The optimized method was successfully applied to the analysis of polyphenols in fruits of L. barbarum L. Additionally, their free radicalscavenging activity was assessed by 1,1-diphenyl-2-picrylhydrazyl radical (DPPH • ).

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Section: Chromatographic Methods Developmentmentioning

confidence: 99%

Chromatographic Determination of Phenolic Acids and Flavonoids in Lycium barbarum L. and Evaluation of Antioxidant Activity

Magiera

Zareba

2015

Food Anal. Methods

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“…1). Pharmacologically active substances require derivatization prior to GC-MS analysis and silylation is the most frequent choice for this purpose [4,11,17,19,23,30,31]. As shown in several studies, a mixture of BSTFA and TMCS can simultaneously silylate amino, alcohol and carboxyl groups in PAS in a single step [23,30,31,33].…”

Section: Optimization Of Microwave-assisted Derivatizationmentioning

confidence: 99%

“…A number of authors have used extraction, sample clean-up and derivatization procedures to facilitate the determination of PAS in various types of biological samples. Such procedures are based on solid-phase extraction (SPE) [4,[10][11][12][13][14][15][16][17][18][19], solid-phase microextraction [20,21], liquid-liquid extraction [13,[22][23][24][25], stir bar sorptive extraction [26,27] and supercritical fluid extraction in combination with solid phase extraction [10,28]. Solid-phase extraction is probably the most popular technique in this context by virtue of its expeditiousness, reproducibility and low cost.…”

Section: Introductionmentioning

confidence: 99%

“…During the last years a series of different polymer-based materials for the SPE of either acidic, neutral and basic compounds out of different sample matrices have been developed [29]. Oasis-HLB is seemingly the most commonly used sorbent for the solid-phase extraction of PAS from biological samples [11,13,14,19,30]. Oasis HLB is a macroporous copolymer consisting of two monomer components, the lipophilic divinylbenzene and the hydrophilic n-vinylpyrrolidone.…”

Section: Introductionmentioning

confidence: 99%

“…In fact, liquid chromatography (LC) has been used in combination with mass spectrometry [12][13][14][15][16]18,20,24,25], UV-visible and diode array detectors [3,13,15,22,26,27] to determine PAS in different types of biological fluids, and so has gas chromatography, mostly in conjunction with mass spectrometers detector (GC-MS) [4,11,17,19,23,28].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Gas chromatography–mass spectrometry determination of pharmacologically active substances in urine and blood samples by use of a continuous solid-phase extraction system and microwave-assisted derivatization

Azzouz

Ballesteros

2012

Journal of Chromatography B

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Chemiluminescence of off‐line and on‐line gold nanoparticle‐catalyzed luminol system in the presence of flavonoid

Zhang

Liu

et al. 2016

Luminescence

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It was found that flavonoids could remarkably inhibit the chemiluminescence (CL) intensity of an off-line gold nanoparticle (AuNP)-catalyzed luminol-H O CL system. By contrast, flavonoids enhanced the CL intensity of an on-line AuNP-catalyzed luminol-H O CL system. In the off-line system, the AuNPs were prepared beforehand, whereas in the on-line system, AuNPs were produced by on-line mixing of luminol prepared in a buffer solution of NaHCO - Na CO and HAuCl with no need for the preliminary preparation of AuNPs. The on-line system had prominent advantages over the off-line system, namely a lowering of the background noise and improvements in the stability of the CL system. The results show that differences in the signal suppression effect of flavonoids on the off-line AuNP-catalyzed CL system are influenced by the combined action of a free radical scavenging effect and occupy-sites function; the latter was proved to be predominant using controlled experiments. Enhancement of the on-line system was ascribed to the presence of flavonoids promoting the on-line formation of AuNPs, which better catalyzed the luminol-H O CL reaction, and the enhancement activity of the six flavonoids increased with the increase in reducibility. This work broadens the scope of practical applications of an AuNP-catalyzed CL system.

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GC–MS method for the simultaneous determination of β-blockers, flavonoids, isoflavones and their metabolites in human urine

Cited by 60 publications

References 40 publications

Chromatographic Determination of Phenolic Acids and Flavonoids in Lycium barbarum L. and Evaluation of Antioxidant Activity

Chromatographic Determination of Phenolic Acids and Flavonoids in Lycium barbarum L. and Evaluation of Antioxidant Activity

Gas chromatography–mass spectrometry determination of pharmacologically active substances in urine and blood samples by use of a continuous solid-phase extraction system and microwave-assisted derivatization

Chemiluminescence of off‐line and on‐line gold nanoparticle‐catalyzed luminol system in the presence of flavonoid

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