GC-MS profiling of volatile metabolites produced by Klebsiella pneumoniae

Filipiak, Wojciech; Żuchowska, Karolina; Marszałek, M.; Depka, Dagmara; Bogiel, Tomasz; Warmuzińska, Natalia; Bojko, Barbara

doi:10.3389/fmolb.2022.1019290

Cited by 18 publications

(31 citation statements)

References 49 publications

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“…In recent years, VOCs have been increasingly utilized for strain characterization (Drees et al 2019 ; Kunze et al 2013 ; Lu et al 2022 ). In addition, it is possible to differentiate CRKP and CSKP in TSB media using changes in VOCs (Filipiak et al 2022 ). Nevertheless, we note that there are few studies employing commercial BC bottles emitting VOCs to identify CRKP and determine its CBPM phenotype, further emphasizing the strengths of the present study.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have demonstrated a correlation between the growth metabolism of K . pneumoniae and VOCs, including propionic acid (BCs) (Julak et al 2000 ), acetic acid (BCs/LB) (Julak et al 2000 ), 3-methyl-1-butanol (sheep blood agar/TSB/LB) (Filipiak et al 2022 ; Junger et al 2012 ; Luo et al 2023 ), butane-2,3-dione (diacetyl) (TSB) (Filipiak et al 2022 ), and 3-hydroxy-2-butanone (malt extract agar and dichloran glycerol agar/TSB supplemented with 5% hemolyzed human blood) (Kiviranta et al 1998 ; Rees et al 2016 ). These findings align with the outcomes of the present study.…”

Section: Discussionmentioning

confidence: 99%

“…In our previous study, we achieved early identification of CBPM-producing CRKP by examining changes in 3-methyl-1-butanol levels in trypticase soy broth (TSB) cultures (Luo et al 2023 ). Furthermore, another study reported that carbapenem-susceptible Klebsiella pneumoniae (CSKP) can be differentiated from CRKP based on VOCs changes in TSB cultures (Filipiak et al 2022 ). However, these studies employed gas chromatography–mass spectrometry (GC–MS), which has multiple pre-processing steps and long MS analysis time, making it unsuitable for widespread adoption.…”

Section: Introductionmentioning

confidence: 99%

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GC–IMS facilitates identification of carbapenem-resistant Klebsiella pneumoniae in simulated blood cultures

Li,

Zheng,

Zhao

et al. 2024

AMB Expr

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This study aimed to identify carbapenem-resistant Klebsiella pneumoniae (CRKP) based on changes in levels of its volatile organic compounds (VOCs) in simulated blood cultures (BCs) using the gas chromatography–ion mobility spectrometry (GC–IMS) technique. A comprehensive analysis of volatile metabolites produced by Klebsiella pneumoniae (K. pneumoniae) in BC bottles was conducted using GC–IMS. Subsequently, the released VOCs were analyzed to examine differences in VOC release between CRKP and carbapenem-susceptible Klebsiella pneumoniae (CSKP). A total of 54 VOCs were detected, of which 18 (6 VOCs found in both monomer and dimer forms) were successfully identified. The VOCs produced by K. pneumoniae in BC bottles (BacT/ALERT® SA) were primarily composed of organic acids, alcohols, esters, and ketones. The content of certain VOCs was significantly different between CRKP and CSKP after the addition of imipenem (IPM). Moreover, the inclusion of carbapenemase inhibitors facilitated the identification of carbapenemase-producing K. pneumoniae based on the variations in VOCs. This study demonstrates the utility of GC–IMS technology in identifying CRKP, and reveals that changes in VOCs are closely related to the growth and metabolism of K. pneumoniae, indicating that they can be leveraged to promote early identification of CRKP bacteremia. However, further in-depth studies and experiments are needed to validate our findings.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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GC–IMS facilitates identification of carbapenem-resistant Klebsiella pneumoniae in simulated blood cultures

Li,

Zheng,

Zhao

et al. 2024

AMB Expr

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“…Benzene is a possible marker for lung carcinoma [58] and 2-nonanone is a possible marker for bacterial infections, especially Pseudomonas aeruginosa and Staph aureus [59] and Klebsiella pneumoniae [60] were also detectable in room air. If only their concentration in exhaled air is considered, inhalation exposure to these markers would be ignored.…”

Section: Runmentioning

confidence: 99%

Alveolar gradients in breath analysis. A pilot study with comparison of room air and inhaled air by simultaneous measurements using ion mobility spectrometry

Westhoff,

Keßler,

Baumbach

2023

J. Breath Res.

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Analyzing exhaled breath samples, especially using a highly sensitive method such as MCC/IMS (multi-capillary column/ion mobility spectrometry), may also detect analytes that are derived from exogenous production. In this regard, there is a discussion about the optimal interpretation of exhaled breath, either by considering volatile organic compounds (VOCs) only in exhaled breath or by additionally considering the composition of room air and calculating the alveolar gradients. However, there are no data on whether the composition and concentration of VOCs in room air are identical to those in truly inhaled air directly before analyzing the exhaled breath. The current study aimed to determine whether the VOCs in room air, which are usually used for the calculation of alveolar gradients, are identical to the VOCs in truly inhaled air. For the measurement of inhaled air and room air, two IMS, each coupled with an MCC that provided a pre-separation of the VOCs, were used in parallel. One device was used for sampling room air and the other for sampling inhaled air. Each device was coupled with a newly invented system that cleaned room air and provided a clean carrier gas, whereas formerly synthetic air had to be used as a carrier gas. In this pilot study, a healthy volunteer underwent three subsequent runs of sampling of inhaled air and simultaneous sampling and analysis of room air. Three of the selected 11 peaks (P4 - unknown, P5 - 1-Butanol, and P9 - Furan, 2-methyl-) had significantly higher intensities during inspiration than in room air, and four peaks (P1 - 1-Propanamine, N-(phenylmethylene), P2 - 2-Nonanone, P3 - Benzene, 1,2,4-trimethyl-, and P11 - Acetyl valeryl) had higher intensities in room air. Furthermore, four peaks (P6 - Benzaldehyde, P7 - Pentane, 2-methyl-, P8 - Acetone, and P10 - 2-Propanamine) showed inconsistent differences in peak intensities between inhaled air and room air. To the best of our knowledge, this is the first study to compare simultaneous sampling of room air and inhaled air using MCC/IMS. The simultaneous measurement of inhaled air and room air showed that using room air for the calculation of alveolar gradients in breath analysis resulted in different alveolar gradient values than those obtained by measuring truly inhaled air.

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“…Gas chromatography combined with mass spectrometry (GC–MS) is usually used to analyze the volatile metabolome ( Filipiak et al, 2022 ). Composition of SCFAs in stool samples are well described but most of the published data were obtained by the solid phase microextraction method (SPME) ( Marrubini et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Investigating volatile compounds in the Bacteroides secretome

Shagaleeva¹,

Kashatnikova²,

Kardonsky³

et al. 2023

Front. Microbiol.

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Microorganisms and their hosts communicate with each other by secreting numerous components. This cross-kingdom cell-to-cell signaling involves proteins and small molecules, such as metabolites. These compounds can be secreted across the membrane via numerous transporters and may also be packaged in outer membrane vesicles (OMVs). Among the secreted components, volatile compounds (VOCs) are of particular interest, including butyrate and propionate, which have proven effects on intestinal, immune, and stem cells. Besides short fatty acids, other groups of volatile compounds can be either freely secreted or contained in OMVs. As vesicles might extend their activity far beyond the gastrointestinal tract, study of their cargo, including VOCs, is even more pertinent. This paper is devoted to the VOCs secretome of the Bacteroides genus. Although these bacteria are highly presented in the intestinal microbiota and are known to influence human physiology, their volatile secretome has been studied relatively poorly. The 16 most well-represented Bacteroides species were cultivated; their OMVs were isolated and characterized by NTA and TEM to determine particle morphology and their concentration. In order to analyze the VOCs secretome, we propose a headspace extraction with GC–MS analysis as a new tool for sample preparation and analysis of volatile compounds in culture media and isolated bacterial OMVs. A wide range of released VOCs, both previously characterized and newly described, have been revealed in media after cultivation. We identified more than 60 components of the volatile metabolome in bacterial media, including fatty acids, amino acids, and phenol derivatives, aldehydes and other components. We found active butyrate and indol producers among the analyzed Bacteroides species. For a number of Bacteroides species, OMVs have been isolated and characterized here for the first time as well as volatile compounds analysis in OMVs. We observed a completely different distribution of VOC in vesicles compared to the bacterial media for all analyzed Bacteroides species, including almost complete absence of fatty acids in vesicles. This article provides a comprehensive analysis of the VOCs secreted by Bacteroides species and explores new perspectives in the study of bacterial secretomes in relation the intercellular communication.

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GC-MS profiling of volatile metabolites produced by Klebsiella pneumoniae

Cited by 18 publications

References 49 publications

GC–IMS facilitates identification of carbapenem-resistant Klebsiella pneumoniae in simulated blood cultures

GC–IMS facilitates identification of carbapenem-resistant Klebsiella pneumoniae in simulated blood cultures

Alveolar gradients in breath analysis. A pilot study with comparison of room air and inhaled air by simultaneous measurements using ion mobility spectrometry

Investigating volatile compounds in the Bacteroides secretome

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