Gc-Ms Study of Two Column Fractions from Methanol Extracts of Loranthus Micranthus and Their In Vivo Antidiabetic Activity on Alloxan Induced Diabetic Rats

Abstract

The methanol extracts of Loranthus micranthus fractions were collected from column chromatography. From TLC, the fraction 17 and 20 were selected based on the clear distiguishable of bands and few phytochemicals. Same fractions when subjected to GC-MS, fraction-17 yielded 13 and fraction-20 yielded 11 prominent compounds. Diabetic rats that were treated with the fraction 17 and 20 showed significantly increase in body weight (16.52 and 12.43 % respectively). Hypoglycemic effect and antihyperlipidemic characters (decrease in the previously elevated levels of serum cholesterol and serum triglycerides) were observed. The two fractions treated rats have also showed the regeneration of pancreatic connective tissue, acini, blood vessels, blood capillaries, pancreatic ducts and their walls, islets capsule and cells in accordance to proper cytoarchitecture. The in vivo antidiabetic property may be due to the presence of single or all phytochemicals in the fraction. Greater activity was observed in fraction-17. However, further experimental studies are still needed for identification of the functionally relevant phytochemical(s) in management of diabetes in rats.sp. were the common host plants of L. micranthus [10]. Herbals are the choice over synthetic drugs from past centuries having multiple therapeutic functions and fewer side effects. In this context it was an effort to attend towards antidibetic studies of L. micranthus in an analytical and systematic ways. L. micranthus has been widely used in ethnomedicine for various purposes, including antihypertensive, anticancer, antispasmodic, and antidiabetic, in treatment of epilepsy, headache, infertility, menopausal syndrome and rheumatism [11,12] and many of medicinal activities are proven by recent researches. It decreases the blood glucose level and controls the loss of body weight in diabetes mellitus [13].Presently the study was aimed to carry out GS-MS and in vivo antidiabetic activities of Loranthus micranthus on alloxan induced diabetic rats. Based on our earlier studies of qualitative, quantitative and some biological activities, column fractions from methanol extracts of L. micranthus were selected for in vivo antidiabetic studies on rats. Body weight, blood glucose level, lipid profile and histopathological changes of rats were considered in the study. Materials and Methods Collection and processing of plantThe fresh leaves of Loranthus micranthus growing on the host plant

Unraveling the chemical profile, antioxidant, enzyme inhibitory, cytotoxic potential of different extracts from Astragalus caraganae

“…[28] In this regard, extracts from medicinal plants and their phytochemicals have been found to play an exceptional role in the management of diabetes, while having lesser side effects. [29][30][31] In the current work, all extracts displayed antidiabetic effect through inhibition of amylase and glucosidase (0.04-0.56 and 0.93-1.09 mmol ACAE/g, respectively) (Table 4). While the less polar extracts showed lower amylase inhibition compared to the polar ones, the polar extracts on the other hand, displayed slightly higher antiglucosidase effect than the less polar ones.…”

“…[ 28 ] In this regard, extracts from medicinal plants and their phytochemicals have been found to play an exceptional role in the management of diabetes, while having lesser side effects. [ 29–31 ]…”

Mechanistic evaluation of phytochemicals in breast cancer remedy: current understanding and future perspectives

“…They have been used since ancient times to cope with various ailments including cancer, diabetes, cardiovascular diseases, inammation, neurological disorders and skin diseases etc. [22][23][24][25][26][27][28] Numerous epidemiological studies have predicted reduced cancer incidence with the use of phytochemicals. [29][30][31] Phytochemicals such as curcumin, 32 resveratrol, 33,34 epigallocatechin gallate (EGCG), 35,36 silibinin, 37 benzyl isothiocyanate, 38,39 genistein, 40 kaempferol, 41,42 thymoquinone, 43 quercetin, 44 parthenolide, 45 sulforaphane, 46 naringenin, 47,327,330 isoliquiritigenin 48,49 and ginsenosides [50][51][52] have been shown to suppress breast carcinoma via modulation of various signalling transduction pathways, genes and gene products.…”