GDF-15 gene expression alterations in human lymphoblastoid cells and peripheral blood lymphocytes following exposure to ionizing radiation

Abstract

The identification of rapid, sensitive and high-throughput biomarkers is imperative in order to identify individuals harmed by radiation accidents, and accurately evaluate the absorbed doses of radiation. DNA microarrays have previously been used to evaluate the alterations in growth/differentiation factor 15 (GDF15) gene expression in AHH-1 human lymphoblastoid cells, following exposure to γ-rays. The present study aimed to characterize the relationship between the dose of ionizing radiation and the produced effects in GDF-15 gene expression in AHH-1 cells and human peripheral blood lymphocytes (HPBLs). GDF-15 mRNA and protein expression levels following exposure to γ-rays and neutron radiation were assessed by reverse transcription-quantitative polymerase chain reaction and western blot analysis in AHH-1 cells. In addition, alterations in GDF-15 gene expression in HPBLs following ex vivo irradiation were evaluated. The present results demonstrated that GDF-15 mRNA and protein expression levels in AHH-1 cells were significantly upregulated following exposure to γ-ray doses ranging between 1 and 10 Gy, regardless of the dose rate. A total of 48 h following exposure to neutron radiation, a dose-response relationship was identified in AHH-1 cells at γ-ray doses between 0.4 and 1.6 Gy. GDF-15 mRNA levels in HPBLs were significantly upregulated following exposure to γ-ray doses between 1 and 8 Gy, within 4–48 h following irradiation. These results suggested that significant time- and dose-dependent alterations in GDF-15 mRNA and protein expression occur in AHH-1 cells and HPBLs in the early phases following exposure to ionizing radiation. In conclusion, alterations in GDF-15 gene expression may have potential as a biomarker to evaluate radiation exposure.

Systemic modulation of stress and immune parameters in patients treated for prostate adenocarcinoma by intensity-modulated radiation therapy or stereotactic ablative body radiotherapy

“…GDF-15 is a member of the TGFβ family with both pro- and anti-tumor activities [ 41 ]. Its expression is associated with cellular stress conditions like irradiation in human PBLs [ 42 ], primary fibroblasts exposed ex vivo [ 43 , 44 ], and murine splenocytes following whole-body exposure [ 45 ]. Our results now show that it is also transiently induced in vivo during RT, with specific regulation in each RT modality.…”

Large-Scale Fabrication of Highly Filled Polymer Fibers for Radiation Protection Safety and Wear Comfort of Multilayer Fabrics in Complex Radiations

“…1,2 We benefit great conveniences from these flourishing technologies, although they bear huge threats in daily life in that radiations have the ability to induce gene mutation and even death. 3,4 It is well known that several nuclear accidents have caused many deaths in recent decades. In particular, Japan government plans to discharge the nuclear wastewater of Fukushima nuclear plants into the sea.…”

“…Complex radiations are composed of α/β particles, X-rays, γ-rays, neutrons, and other high energy particles, which widely exist in diverse industries across nuclear power plants, health care, and aerospace. , We benefit great conveniences from these flourishing technologies, although they bear huge threats in daily life in that radiations have the ability to induce gene mutation and even death. , It is well known that several nuclear accidents have caused many deaths in recent decades. In particular, Japan government plans to discharge the nuclear wastewater of Fukushima nuclear plants into the sea. , Namely, not only nuclear personnel but also the public across the world is faced with the increasing risk of direct exposure to radiations .…”

Role of blood derived cell fractions, temperature and sample transport on gene expression-based biological dosimetry

“…All these studies have identified sets of responsive genes in human PBMCs, whole blood or WBC (Kabacik, Mackay, et al 2011;Manning et al 2013;Beer et al 2014;Chauhan and Howland 2014;Li et al 2017). Some being cross validated in vivo in other species (Port et al 2018).…”