GDF-15 Level Correlates with CMKLR1 and VEGF-A in Tumor-free Margin in Colorectal Cancer

Mielcarska, Sylwia; Stopińska, Kamila; Dawidowicz, Miriam; Kula, Agnieszka; Kiczmer, Paweł; Seńkowska, Alicja Prawdzic; Nowakowska-Zajdel, Ewa; Walkiewicz, Katarzyna; Waniczek, Dariusz; Świętochowska, Elżbieta

doi:10.1007/s11596-021-2335-0

Cited by 12 publications

(10 citation statements)

References 21 publications

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“…Our recent studies confirmed the positive correlation between CMKLR1 and MMP-9 (Matrix metallopeptidase 9), which is involved in angiogenesis by remodeling the extracellular matrix [16]. However, in our previous study [20], we did not observe any association between CMKLR1 and VEGF A levels in either the tumor or the margin tissue of CRC. In this study, we assessed the influence of CMKLR on MVD at the invasive front of the tumor.…”

Section: Discussionsupporting

confidence: 52%

“…Upregulation of CMKLR ligand, chemerin, was found in coronary arteries undergoing hypoxia [26]. In our previous study [20], we observed a positive correlation between levels of CMKLR1 and HIF-1α (hypoxia-inducible factor 1) in margin tissues of CRC. Therefore, we may suspect that CMKLR1 is upregulated in poorly vascularized tumors, probably in response to a hypoxic microenvironment.…”

Section: Discussionmentioning

confidence: 74%

“…Further studies should be performed to precisely determine the role of CMKLR in EMT and angiogenesis. Based on previous studies, we may suspect that upregulation of CMKLR in tumors may be associated with poor vasculature and hypoxia [20]. To the best of our knowledge, tumor budding is related to poorly developed vasculature.…”

Section: Discussionmentioning

confidence: 95%

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The Concentration of CMKLR1 Expression on Clinicopathological Parameters of Colorectal Cancer: A Preliminary Study

et al. 2021

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Background and Objectives: Colorectal cancer (CRC) is the second-most common cause of cancer-related deaths worldwide. Angiogenesis is crucial for cancer growth, infiltration of surrounding tissues, and metastasis and plays a key role in the pathogenesis of CRC. Chemerin/chemokine-like receptor 1 (CMKLR1) is one of the biochemical pathways involved in the regulation of angiogenesis in solid tumors. The aim of the study was to assess the CMKLR1 level in tumor and margin tissues of CRC in relation to histopathological parameters: microvessel density (MVD), budding, tumor-infiltrating lymphocytes (TILs), TNM scale, and grading. Materials and Methods: The study involved 43 samples of tumor and margin tissues obtained from CRC patients. To assess the concentration of CMKLR1 a commercially available enzyme-linked immunosorbent assay kit was used. For 35 cases, we performed CD34 immunostaining. The MVD, budding, and TILs were assessed using a light microscope. Results: The levels of CMKLR1 in both tumor and margin were negatively correlated with MVD and budding. CMKLR1 concentration in margin was higher in tissues with lymphocytic infiltration. Conclusions: Low vascularity and low budding are associated with higher CMKLR1 expression. CMKLR1 might play a multifunctional role in CRC pathogenesis by influencing tumor budding and peritumoral lymphocytic infiltration.

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Section: Discussionsupporting

confidence: 52%

Section: Discussionmentioning

confidence: 74%

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The Concentration of CMKLR1 Expression on Clinicopathological Parameters of Colorectal Cancer: A Preliminary Study

et al. 2021

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“…Serum VEGF scales of all sufferers are detected by enzyme-linked immunosorbent assay (ELISA), and miR-323and miR-409-3p scales are detected by real-time quantitative PCR (qPCR) [ 25 ]. Contrast-enhanced MRI scans are also performed in both sets [ 26 ]. There is no extensive disparity in the baseline data of the two sets of sufferers ( P > 0.05), which is feasible.…”

Section: Patients Information and Research Methodsmentioning

confidence: 99%

Investigation of the Disparities in Ultrasound Imaging Features of miR-323, miR-409-3p, and VEGF Expression Scales in Different Clinicopathological Features of Prostate Carcinoma and Their Correlation with Prognosis

Liu

Wang

Cui

et al. 2022

BioMed Research International

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Prostate carcinoma (PC) is a disease of the male genitourinary system and a relatively common malignant tumor. In order to investigate the disparities in the expression of microRNA-323 (miR-323), microRNA-409-3p (miR-409-3p), and vascular endothelial growth factor (VEGF) in prostate carcinoma with different clinicopathological features and analyze their correlation with prognosis. Thirty-two sufferers with prostate carcinoma and forty-three sufferers with benign prostatic hyperplasia are included. The results show that the detection of miR-323, miR-409-3p, and VEGF scales can provide reference value for clinical guidance of prostate carcinoma prognosis.

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“…In CRC, CMKLR1 was discovered at higher abundance in CRC tumor tissues than in margins [ 30 ]. In addition, CMKLR1 was found to correlate with GDF-15 and VEGF-A levels in CRC tumor-free margin [ 33 ]. Our own previous report revealed CMKLR1 to be highly expressed in the breast cancer cell line DU4475 [ 31 ].…”

Section: Discussionmentioning

confidence: 99%

A Chemerin Peptide Analog Stimulates Tumor Growth in Two Xenograft Mouse Models of Human Colorectal Carcinoma

Friebus‐Kardash

Schulz

Reinicke

et al. 2021

Cancers

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Background: Chemerin plasma concentration has been reported to be positively correlated with the risk of colorectal cancer. However, the potential regulation of CRC tumorigenesis and progression has not yet been investigated in an experimental setting. This study addresses this hypothesis by investigating proliferation, colony formation, and migration of CRC cell lines in vitro as well as in animal models. Methods: In vitro, microscopic assays to study proliferation, as well as a scratch-wound assay for migration monitoring, were applied using the human CRC cell lines HCT116, HT29, and SW620 under the influence of the chemerin analog CG34. The animal study investigated HCT116-luc and HT29-luc subcutaneous tumor size and bioluminescence during treatment with CG34 versus control, followed by an ex-vivo analysis of vessel density and mitotic activity. Results: While the proliferation of the three CRC cell lines in monolayers was not clearly stimulated by CG34, the chemerin analog promoted colony formation in three-dimensional aggregates. An effect on cell migration was not observed. In the treatment study, CG34 significantly stimulated both growth and bioluminescence signals of HCT116-luc and HT29-luc xenografts. Conclusions: The results of this study represent the first indication of a tumor growth-stimulating effect of chemerin signaling in CRC.

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GDF-15 Level Correlates with CMKLR1 and VEGF-A in Tumor-free Margin in Colorectal Cancer

Cited by 12 publications

References 21 publications

The Concentration of CMKLR1 Expression on Clinicopathological Parameters of Colorectal Cancer: A Preliminary Study

The Concentration of CMKLR1 Expression on Clinicopathological Parameters of Colorectal Cancer: A Preliminary Study

Investigation of the Disparities in Ultrasound Imaging Features of miR-323, miR-409-3p, and VEGF Expression Scales in Different Clinicopathological Features of Prostate Carcinoma and Their Correlation with Prognosis

A Chemerin Peptide Analog Stimulates Tumor Growth in Two Xenograft Mouse Models of Human Colorectal Carcinoma

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