GDF15 induced by compressive force contributes to osteoclast differentiation in human periodontal ligament cells

Li, Shuo; Li, Qian; Zhu, Ye; Hu, Wei

doi:10.1016/j.yexcr.2019.111745

Cited by 34 publications

(41 citation statements)

References 39 publications

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“…Whereas soluble OPG molecules bind to RANKL to block the binding of RANKL to RANK, thereby preventing osteoclast formation and maturation [36]. Interestingly, compressive force induced the upregulatino of RANKL/OPG ratio in human periodontal ligament cells [37]. Similarly, the in vitro experiments suggested that silencing OPG reversed the role of proper stress on the differentiation of ex vivo cells of femoral head.…”

Section: Discussionmentioning

confidence: 99%

Proper mechanical stress promotes femoral head recovery from steroid-induced osteonecrosis in rats through the OPG/RANK/RANKL system

Qin

Yang

et al. 2020

BMC Musculoskelet Disord

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Background: Long-term use of steroid may lead to osteonecrosis of the femoral head (ONFH). Mechanical stress may help bone formation and remodeling. This study aimed to probe the role of mechanical stress in the femoral head recovery in rats. Methods: Rat models with ONFH were induced by steroid. Rats were subjected to different levels of mechanical stress (weight-bearing training), and then the morphology and bone density of femoral head of rats were measured. The mRNA and protein levels of the OPG/RANK/RANKL axis in rat femoral head were assessed. Gain-and loss-of function experiments of OPG were performed to identify its role in femoral head recovery following stress implement. The ex vivo cells were extracted and the effects of stress and OPG on osteogenesis in vitro were explored. Results: Steroid-induced ONFH rats showed decreased bone density and increased bone spaces, as well as necrotic cell colonies and many cavities in the cortical bones and trabeculars. Proper mechanical stress or upregulation of OPG led to decreased RANK/RANKL expression and promoted femoral head recovery from steroid-induced osteonecrosis. However, excessive mechanical stress might impose too much load on the femurs thus leading even retard femoral head recovery process. In addition, the in vitro experimental results supported that proper stress and overexpression of OPG increased the osteogenesis of ex vivo cells of femoral head. Conclusion: This study provided evidence that proper mechanical stress promoted femoral head recovery from steroid-induced osteonecrosis through the OPG/RANK/RANKL system, while overload might inhibit the recovery process. This study may offer novel insights for ONFH treatment.

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Section: Discussionmentioning

confidence: 99%

Proper mechanical stress promotes femoral head recovery from steroid-induced osteonecrosis in rats through the OPG/RANK/RANKL system

Qin

Yang

et al. 2020

BMC Musculoskelet Disord

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“…It has been proposed that tensile force associated with cytoskeletal organization leads to Yap nuclear localization and activation, promoting cell proliferation and osteogenic differentiation 21 . Meanwhile, compressive force can result in decreased expression of Yap and facilitate osteoclast formation 10 . However, the mechanism of how specific PDLC subpopulation respond to orthodontic force in vivo remains unclear.…”

Section: Discussionmentioning

confidence: 99%

“…Given the human periodontal ligament stromal/stem cells (hPDLSCs) are obtained and cultured easily in vitro, they have been intensively evaluated to partly elucidate the mechanism of OTM 7,8 . It has been proposed that hPDLSCs respond to mechanical force including differentiating into osteoblasts under tensile stress 5 and promoting maturation of osteoclasts under compressive stress 9,10 . However, there are limited functional evidence about how PDLC subpopulations participate in force‐induced bone remodelling.…”

Section: Introductionmentioning

confidence: 99%

Mechanosensing by Gli1⁺ cells contributes to the orthodontic force‐induced bone remodelling

et al. 2020

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Objectives: Gli1 + cells have received extensive attention in tissue homeostasis and injury mobilization. The aim of this study was to investigate whether Gli1 + cells respond to force and contribute to bone remodelling. Materials and methods:We established orthodontic tooth movement (OTM) model to assess the bone response for mechanical force. The transgenic mice were utilized to label and inhibit Gli1 + cells, respectively. Additionally, mice that conditional ablate Yes-associated protein (Yap) in Gli1 + cells were applied in the present study. The tooth movement and bone remodelling were analysed. Results:We first found Gli1 + cells expressed in periodontal ligament (PDL). They were proliferated and differentiated into osteoblastic cells under tensile force. Next, both pharmacological and genetic Gli1 inhibition models were utilized to confirm that inhibition of Gli1 + cells led to arrest of bone remodelling. Furthermore, immunofluorescence staining identified classical mechanotransduction factor Yap expressed in Gli1 + cells and decreased after suppression of Gli1 + cells. Additionally, conditional ablation of Yap gene in Gli1 + cells inhibited the bone remodelling as well, suggesting Gli1 + cells are force-responsive cells. Conclusions:Our findings highlighted that Gli1 + cells in PDL directly respond to orthodontic force and further mediate bone remodelling, thus providing novel functional evidence in the mechanism of bone remodelling and first uncovering the mechanical responsive property of Gli1 + cells.

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“…The periodontal ligament is a layer of connective tissue between the root of the tooth and the alveolar bone 40,41 . PDLCs contribute to the regeneration of periodontal ligament, cementum and alveolar bone 42,43 and are recognized as the histological basis for OTM 29,44,45 . However, previous studies explaining the bone‐specific anabolic property of SMV mostly based on observation of static‐culturing osteoblasts 46,47 .…”

Section: Discussionmentioning

confidence: 99%

Local delivery of simvastatin maintains tooth anchorage during mechanical tooth moving via anti‐inflammation property and AMPK/MAPK/NF‐kB inhibition

Sun

Zhu

et al. 2020

J Cellular Molecular Medi

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GDF15 induced by compressive force contributes to osteoclast differentiation in human periodontal ligament cells

Cited by 34 publications

References 39 publications

Proper mechanical stress promotes femoral head recovery from steroid-induced osteonecrosis in rats through the OPG/RANK/RANKL system

Proper mechanical stress promotes femoral head recovery from steroid-induced osteonecrosis in rats through the OPG/RANK/RANKL system

Mechanosensing by Gli1⁺ cells contributes to the orthodontic force‐induced bone remodelling

Local delivery of simvastatin maintains tooth anchorage during mechanical tooth moving via anti‐inflammation property and AMPK/MAPK/NF‐kB inhibition

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GDF15 induced by compressive force contributes to osteoclast differentiation in human periodontal ligament cells

Cited by 34 publications

References 39 publications

Proper mechanical stress promotes femoral head recovery from steroid-induced osteonecrosis in rats through the OPG/RANK/RANKL system

Proper mechanical stress promotes femoral head recovery from steroid-induced osteonecrosis in rats through the OPG/RANK/RANKL system

Mechanosensing by Gli1+ cells contributes to the orthodontic force‐induced bone remodelling

Local delivery of simvastatin maintains tooth anchorage during mechanical tooth moving via anti‐inflammation property and AMPK/MAPK/NF‐kB inhibition

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Mechanosensing by Gli1⁺ cells contributes to the orthodontic force‐induced bone remodelling