GDNF-pretreatment enhances the survival of neural stem cells following transplantation in a rat model of Parkinson's disease

Wang, F.; Kameda, Masahiro; Yasuhara, Takao; Tajiri, Naoki; Kikuchi, Yoichiro; Liang, Hanbai; Tayra, Judith Thomas; Shinko, Aiko; Wakamori, Takaaki; Agari, Takashi; Date, Isao

doi:10.1016/j.neures.2011.05.019

Cited by 35 publications

(28 citation statements)

References 25 publications

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“…3,4 Therefore, there are now active pursuits in the eld of tissue engineering to utilize biomaterials as scaffolds to assist stem cell survival and guided integration along with supplying sustained and localized biomolecules to injured CNS sites. [5][6][7] The eld of tissue engineering combines scaffolds, cells and regulatory molecules to create ideal bio-mimic microenvironments for restoring and regenerating injured body tissues. Currently, the materials used for fabrication of scaffolds in the eld mainly focused on naturally derived or synthetic ones.…”

Section: Introductionmentioning

confidence: 99%

Chemically modified electrospun silica nanofibers for promoting growth and differentiation of neural stem cells

Chen

Hsieh²,

Yang

et al. 2014

J. Mater. Chem. B

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In this study, pure silica nanofibers (SNFs) were fabricated by the electrospinning technique. Subsequently, the as-prepared SNFs were modified with (3-aminopropyl) trimethoxysilane (APTS) for applications in neural tissue engineering. The structure and properties of the as-prepared SNFs and the modified SNFs (SNFAPxM, x ¼ 1-3) were evaluated with FTIR, TGA, nitrogen adsorption/desorption isotherms, and SEM. It was found that the surface hydrophilicity of SNF-APxM was lowered upon increasing the amino alkyl group content. The SEM and confocal images revealed that neural stem cells (NSCs) cultured on the electrospun SNFs and SNF-APxM substrates were elongated along the fibers in comparison to poly-Dlysine-coated (PDL-coated) substrate. In addition, a higher degree of proliferation and more responsive cells were observed for the NSCs cultured on the SNF-AP3M substrate than those on the SNFs and the PDL-coated substrates. The results indicated that the APTS-modified silica nanofibers can be potential substrates for regulating growth and differentiation of NSCs in culture.

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Section: Introductionmentioning

confidence: 99%

Chemically modified electrospun silica nanofibers for promoting growth and differentiation of neural stem cells

Chen

Hsieh²,

Yang

et al. 2014

J. Mater. Chem. B

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“…Parkinson's disease is resulted from the loss of dopaminergic neurons in the substantia nigra1821. Stem cells express glial-cell-line-derived neurotrophic factor, which has been shown to improve the survival and function of dopaminergic neurons that may be one approach to stop the death of dopaminergic neurons2022. Furthermore, stem cells can generate cholinergic neurons to improve the cognitive function of Alzheimer's disease patients1819.…”

mentioning

confidence: 99%

Labeling of neuronal differentiation and neuron cells with biocompatible fluorescent nanodiamonds

Hsu

Liu

Chang

et al. 2014

Sci Rep

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Nanodiamond is a promising carbon nanomaterial developed for biomedical applications. Here, we show fluorescent nanodiamond (FND) with the biocompatible properties that can be used for the labeling and tracking of neuronal differentiation and neuron cells derived from embryonal carcinoma stem (ECS) cells. The fluorescence intensities of FNDs were increased by treatment with FNDs in both the mouse P19 and human NT2/D1 ECS cells. FNDs were taken into ECS cells; however, FNDs did not alter the cellular morphology and growth ability. Moreover, FNDs did not change the protein expression of stem cell marker SSEA-1 of ECS cells. The neuronal differentiation of ECS cells could be induced by retinoic acid (RA). Interestingly, FNDs did not affect on the morphological alteration, cytotoxicity and apoptosis during the neuronal differentiation. Besides, FNDs did not alter the cell viability and the expression of neuron-specific marker β-III-tubulin in these differentiated neuron cells. The existence of FNDs in the neuron cells can be identified by confocal microscopy and flow cytometry. Together, FND is a biocompatible and readily detectable nanomaterial for the labeling and tracking of neuronal differentiation process and neuron cells from stem cells.

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“…Currently, many different types of stem cell can be cultured and transplanted, including induced pluripotent stem cells (iPS cells) (Takahashi & Yamanaka, 2006), embryonic stem cells (ES cells) (Wang et al, 2011), neural stem cells (NSCs) (Kameda et al, 2007;Muraoka et al, 2006) , mesenchymal stem cells (MSCs) (Kurozumi et al, 2004;Wang et al, 2010) , and hematopoietic stem cells (Shyu et al, 2006), and some of these have already been used in clinical trials. Every type of stem cell has unique advantages and disadvantages.…”

Section: Approaches To Regenerative Medicine For Cerebral Infarctionmentioning

confidence: 99%

“…During transplantation, stem cells are subject to hypoxic-ischemic injury. Wang et al showed that graft survival was enhanced by pretreatment with GDNF for three days before NSC transplantation (Wang et al, 2011). Because brain tissue architecture is disrupted in the ischemic brain, the use of biodegradable scaffolds may help transplanted stem cells regenerate and/or restore damaged brain structures and functions, by affecting cell differentiation, morphology, adhesion, or gene expression (Kleinman & Martin, 2005;Steindler, 2002).…”

Section: Graft Survivalmentioning

confidence: 99%

Regenerative Medicine for Cerebral Infarction

Kameda¹,

Date²

2011

Advances in Regenerative Medicine

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GDNF-pretreatment enhances the survival of neural stem cells following transplantation in a rat model of Parkinson's disease

Cited by 35 publications

References 25 publications

Chemically modified electrospun silica nanofibers for promoting growth and differentiation of neural stem cells

Chemically modified electrospun silica nanofibers for promoting growth and differentiation of neural stem cells

Labeling of neuronal differentiation and neuron cells with biocompatible fluorescent nanodiamonds

Regenerative Medicine for Cerebral Infarction

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