GDSL LIPASE1 Modulates Plant Immunity through Feedback Regulation of Ethylene Signaling

Kim, Hye Gi; Kwon, Sung-Kyu; Jang, Young Jin; Nam, Myung Hee; Chung, Joo Hee; Na, Yun-Cheol; Guo, Hongwei; Park, Ohkmae K.

doi:10.1104/pp.113.225649

Cited by 52 publications

(73 citation statements)

References 72 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It was reported recently that EIN3 is greatly elevated in expression and transcriptional activity during leaf senescence and accelerates senescence by repressing miR164 through direct binding to the miR164 promoter (Li et al ., ). We previously demonstrated that GDSL lipase 1 (GLIP1) promotes EIN3 degradation, thereby downregulating EIN3 (Kim et al ., ). The decrease in miR164 levels in the glip1‐1 mutant, as shown in microarray analysis of miRNAs and qRT‐PCR expression analysis, may be explained by GLIP1‐mediated EIN3 downregulation (Kim et al ., ) and EIN3‐mediated miR164 downregulation (Li et al ., ).…”

Section: Discussionmentioning

confidence: 97%

“…We previously demonstrated that GDSL lipase 1 (GLIP1) promotes EIN3 degradation, thereby downregulating EIN3 (Kim et al ., ). The decrease in miR164 levels in the glip1‐1 mutant, as shown in microarray analysis of miRNAs and qRT‐PCR expression analysis, may be explained by GLIP1‐mediated EIN3 downregulation (Kim et al ., ) and EIN3‐mediated miR164 downregulation (Li et al ., ).…”

Section: Discussionmentioning

confidence: 97%

“…Arabidopsis GDSL lipase 1 (GLIP1) was found to be an important regulator of plant immune responses (Kwon et al ., ; Kim et al ., , ). Here, the expression of miR164c , identified by microarray analysis, was correlated with GLIP1 .…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

An Arabidopsis NAC transcription factor NAC4 promotes pathogen‐induced cell death under negative regulation by microRNA164

et al. 2016

Self Cite

View full text Add to dashboard Cite

Hypersensitive response (HR) is a form of programmed cell death (PCD) and the primary immune response that prevents pathogen invasion in plants. Here, we show that a microRNAmiR164 and its target gene NAC4 (At5g07680), encoding a NAC transcription factor, play essential roles in the regulation of HR PCD in Arabidopsis thaliana. Cell death symptoms were noticeably enhanced in NAC4-overexpressing (35S:NAC4) and mir164 mutant plants in response to avirulent bacterial pathogens. NAC4 expression was induced by pathogen infection and negatively regulated by miR164 expression. NAC4-binding DNA sequences were determined by in vitro binding site selection using random oligonucleotide sequences. Microarray, chromatin immunoprecipitation and quantitative real time polymerase chain reaction (qRT-PCR) analyses, followed by cell death assays in protoplasts, led to the identification of NAC4 target genes LURP1, WRKY40 and WRKY54, which act as negative regulators of cell death. Our results suggest that NAC4 promotes hypersensitive cell death by suppressing its target genes and this immune process is fine-tuned by the negative action of miR164.

show abstract

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 97%

See 1 more Smart Citation

An Arabidopsis NAC transcription factor NAC4 promotes pathogen‐induced cell death under negative regulation by microRNA164

et al. 2016

Self Cite

View full text Add to dashboard Cite

show abstract

“…Total and free SA were quantified as previously described (Kim et al , ; Quentin et al , ). Leaf samples (150 mg), together with o ‐anisic acid (50 ng) as an internal standard, were ground in liquid nitrogen and extracted with 90% methanol.…”

Section: Methodsmentioning

confidence: 99%

Lignin‐based barrier restricts pathogens to the infection site and confers resistance in plants

et al. 2019

Self Cite

View full text Add to dashboard Cite

Pathogenic bacteria invade plant tissues and proliferate in the extracellular space. Plants have evolved the immune system to recognize and limit the growth of pathogens. Despite substantial progress in the study of plant immunity, the mechanism by which plants limit pathogen growth remains unclear. Here, we show that lignin accumulates in Arabidopsis leaves in response to incompatible interactions with bacterial pathogens in a manner dependent on Casparian strip membrane domain protein (CASP)‐like proteins (CASPLs). CASPs are known to be the organizers of the lignin‐based Casparian strip, which functions as a diffusion barrier in roots. The spread of invading avirulent pathogens is prevented by spatial restriction, which is disturbed by defects in lignin deposition. Moreover, the motility of pathogenic bacteria is negatively affected by lignin accumulation. These results suggest that the lignin‐deposited structure functions as a physical barrier similar to the Casparian strip, trapping pathogens and thereby terminating their growth.

show abstract

“…Genes belonging to PF00067 were up-regulated in all pathosystems and this Pfam comprises the cytochrome P450 protein family, which includes members involved in the synthesis of plant defence compounds. Genes encoding members of the GDSL-like lipase family (PF00657) were also upregulated in all pathosystems and are required for systemic ethylene-mediated resistance (Kim et al, 2013;Kwon et al, 2009).…”

Section: Similar Transcript Regulation In Selected Host-potyvirus Andmentioning

confidence: 99%

Molecular insights into Cassava brown streak virus susceptibility and resistance by profiling of the early host response

Anjanappa

Mehta

Okoniewski

et al. 2017

Molecular Plant Pathology

View full text Add to dashboard Cite

Cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV) are responsible for significant cassava yield losses in eastern sub-Saharan Africa. To study the possible mechanisms of plant resistance to CBSVs, we inoculated CBSV-susceptible and CBSV-resistant cassava varieties with a mixed infection of CBSVs using top-cleft grafting. Transcriptome profiling of the two cassava varieties was performed at the earliest time point of full infection (28 days after grafting) in the susceptible scions. The expression of genes encoding proteins in RNA silencing, salicylic acid pathways and callose deposition was altered in the susceptible cassava variety, but transcriptional changes were limited in the resistant variety. In total, the expression of 585 genes was altered in the resistant variety and 1292 in the susceptible variety. Transcriptional changes led to the activation of β-1,3-glucanase enzymatic activity and a reduction in callose deposition in the susceptible cassava variety. Time course analysis also showed that CBSV replication in susceptible cassava induced a strong up-regulation of RDR1, a gene previously reported to be a susceptibility factor in other potyvirus-host pathosystems. The differences in the transcriptional responses to CBSV infection indicated that susceptibility involves the restriction of callose deposition at plasmodesmata. Aniline blue staining of callose deposits also indicated that the resistant variety displays a moderate, but significant, increase in callose deposition at the plasmodesmata. Transcriptome data suggested that resistance does not involve typical antiviral defence responses (i.e. RNA silencing and salicylic acid). A meta-analysis of the current RNA-sequencing (RNA-seq) dataset and selected potyvirus-host and virus-cassava RNA-seq datasets revealed that the conservation of the host response across pathosystems is restricted to genes involved in developmental processes.

show abstract

GDSL LIPASE1 Modulates Plant Immunity through Feedback Regulation of Ethylene Signaling

Cited by 52 publications

References 72 publications

An Arabidopsis NAC transcription factor NAC4 promotes pathogen‐induced cell death under negative regulation by microRNA164

An Arabidopsis NAC transcription factor NAC4 promotes pathogen‐induced cell death under negative regulation by microRNA164

Lignin‐based barrier restricts pathogens to the infection site and confers resistance in plants

Molecular insights into Cassava brown streak virus susceptibility and resistance by profiling of the early host response

Contact Info

Product

Resources

About