Gear-shaped carbonate apatite granules with a hexagonal macropore for rapid bone regeneration

Hayashi, Koichiro; Yanagisawa, Toshiki; Kishida, Reiji; Tsuchiya, Akira; Ishikawa, Kunio

doi:10.1016/j.csbj.2023.03.053

Cited by 9 publications

(6 citation statements)

References 92 publications

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“…The volume percentages of new bone in the defect in the empty and CAp-CB scaffold-implanted groups were 21.5 ± 4.6 and 0.5 ± 0.2% at four weeks and 23.7 ± 7.5 and 0.6 ± 0.3% at twelve weeks, respectively. The new bone volume in the CAp-CB scaffold-implanted groups at four and twelve weeks was equal to that in the normal bone (20-30%) [46]. Histological images clarified that fibrous tissues filled the bone defect four weeks after implantation in the empty group (Figure 8A), which coincides with the results in the same bone defect model [47].…”

Section: In Vivo Evaluations Of Bone Formationsupporting

confidence: 80%

“…Moreover, Hayashi et al reported bone formation in clinically used dense CAp granules of different sizes using the same bone defect model as that used in this study [26,46,47,52]. At week four after implantation, the bone percentages in the defects implanted with clinically used dense Cap granules that were 1-2, 0.6-1, and 0.3-0.6 mm in size were approximately 12% [45,52], 20% [26], and 21% [46], respectively. Thus, CAp-CB scaffolds can regenerate bone faster than clinically used dense CAp granules.…”

Section: In Vivo Evaluations Of Bone Formationmentioning

confidence: 53%

Section: In Vivo Evaluations Of Bone Formationmentioning

confidence: 81%

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Lamellar Septa-like Structured Carbonate Apatite Scaffolds with Layer-by-Layer Fracture Behavior for Bone Regeneration

Taleb Alashkar,

Hayashi,

Ishikawa

2024

Biomimetics

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Generally, ceramics are brittle, and porosity is inversely correlated with strength, which is one of the challenges of ceramic scaffolds. Here, we demonstrate that lamellar septum-like carbonate apatite scaffolds have the potential to overcome these challenges. They were fabricated by exploiting the cellular structure of the cuttlebone, removing the organic components from the cuttlebone, and performing hydrothermal treatment. Scanning electron microscopy revealed that the scaffolds had a cellular structure with walls between lamellar septa. The interwall and interseptal sizes were 80–180 and 300–500 μm, respectively. The size of the region enclosed by the walls and septa coincided with the macropore size detected by mercury intrusion porosimetry. Although the scaffold porosity was extremely high (93.2%), the scaffold could be handled without disintegration. The compressive stress–strain curve demonstrated that the scaffolds showed layer-by-layer fracture behavior, which seemed beneficial for avoiding catastrophic failure under impact. When the scaffolds were implanted into rabbit femurs, new bone and blood vessels formed within the scaffold cells at 4 weeks. At 12 weeks, the scaffolds were almost entirely replaced with new bone. Thus, the lamellar septum-like cellular-structured carbonate apatite is a promising scaffold for achieving early bone regeneration and compression resistance.

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Section: In Vivo Evaluations Of Bone Formationsupporting

confidence: 80%

Section: In Vivo Evaluations Of Bone Formationmentioning

confidence: 53%

Section: In Vivo Evaluations Of Bone Formationmentioning

confidence: 81%

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Lamellar Septa-like Structured Carbonate Apatite Scaffolds with Layer-by-Layer Fracture Behavior for Bone Regeneration

Taleb Alashkar,

Hayashi,

Ishikawa

2024

Biomimetics

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“…Among these, porous scaffolds and granules have been used in filling various bone defects; however, in clinical practice, the size and shape of the bone defect cavities often vary and are irregular. The customized bioceramic scaffolds tend to have a regular and orderly shape, and easily adapt to large-sized bone defects, but bioceramic granules can fill random bone defect cavities to meet a wider range of clinical applications ( CHOI et al, 2014 ; Dong et al, 2022 ; Huang et al, 2022 ; Hayashi et al, 2023 ). Unfortunately, the osteoconductive capability of the two types of implants with similar internal pore architectures has not yet been designed for comparison.…”

Section: Discussionmentioning

confidence: 99%

Comparison of osteogenic capability of 3D-printed bioceramic scaffolds and granules with different porosities for clinical translation

Yue,

Zhao,

Yang

et al. 2023

Front. Bioeng. Biotechnol.

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Pore parameters, structural stability, and filler morphology of artificial implants are key factors influencing the process of bone tissue repair. However, the extent to which each of these factors contributes to bone formation in the preparation of porous bioceramics is currently unclear, with the two often being coupled. Herein, we prepared magnesium-doped wollastonite (Mg-CSi) scaffolds with 57% and 70% porosity (57-S and 70-S) via a 3D printing technique. Meanwhile, the bioceramic granules (57-G and 70-G) with curved pore topography (IWP) were prepared by physically disrupting the 57-S and 70-S scaffolds, respectively, and compared for in vivo osteogenesis at 4, 10, and 16 weeks. The pore parameters and the mechanical and biodegradable properties of different porous bioceramics were characterized systematically. The four groups of porous scaffolds and granules were then implanted into a rabbit femoral defect model to evaluate the osteogenic behavior in vivo. 2D/3D reconstruction and histological analysis showed that significant bone tissue production was visible in the central zone of porous granule groups at the early stage but bone tissue ingrowth was slower in the porous scaffold groups. The bone tissue regeneration and reconstruction capacity were stronger after 10 weeks, and the porous architecture of the 57-S scaffold was maintained stably at 16 weeks. These experimental results demonstrated that the structure-collapsed porous bioceramic is favorable for early-stage osteoconduction and that the 3D topological scaffolds may provide more structural stability for bone tissue growth for a long-term stage. These findings provide new ideas for the selection of different types of porous bioceramics for clinical bone repair.

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“…The importance of pore connectivity in scaffolds for bone regeneration is widely recognized. − A structure with high pore connectivity includes a honeycomb structure with continuous uniaxial pores, i.e., channels. ,,− Previous studies demonstrated that honeycomb scaffolds with an adequate channel aperture (∼300 μm) can prevent the intrusion of soft tissues around a bone defect and guide only bone into the scaffold. − A comparison with previous studies revealed that honeycomb scaffolds show superior osteogenesis and angiogenesis in three-dimensional (3D) porous scaffolds. − …”

Section: Introductionmentioning

confidence: 99%

Superiority of Triply Periodic Minimal Surface Gyroid Structure to Strut-Based Grid Structure in Both Strength and Bone Regeneration

Hayashi

Kishida

Tsuchiya

et al. 2023

ACS Appl. Mater. Interfaces

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The aging population has rapidly driven the demand for bone regeneration. The pore structure of a scaffold is a critical factor that affects its mechanical strength and bone regeneration. Triply periodic minimal surface gyroid structures similar to the trabecular bone structure are considered superior to strut-based lattice structures (e.g., grids) in terms of bone regeneration. However, at this stage, this is only a hypothesis and is not supported by evidence. In this study, we experimentally validated this hypothesis by comparing gyroid and grid scaffolds composed of carbonate apatite. The gyroid scaffolds possessed compressive strength approximately 1.6-fold higher than that of the grid scaffolds because the gyroid structure prevented stress concentration, whereas the grid structure could not. The porosity of gyroid scaffolds was higher than that of the grid scaffolds; however, porosity and compressive strength generally have a trade-off relationship. Moreover, the gyroid scaffolds formed more than twice the amount of bone as grid scaffolds in a critical-sized bone defect in rabbit femur condyles. This favorable bone regeneration using gyroid scaffolds was attributed to the high permeability (i.e., larger volume of macropores or porosity) and curvature profile of the gyroid structure. Thus, this study validated the conventional hypothesis using in vivo experiments and revealed factors that led to this hypothetical outcome. The findings of this study are expected to contribute to the development of scaffolds that can achieve early bone regeneration without sacrificing the mechanical strength.

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Gear-shaped carbonate apatite granules with a hexagonal macropore for rapid bone regeneration

Cited by 9 publications

References 92 publications

Lamellar Septa-like Structured Carbonate Apatite Scaffolds with Layer-by-Layer Fracture Behavior for Bone Regeneration

Lamellar Septa-like Structured Carbonate Apatite Scaffolds with Layer-by-Layer Fracture Behavior for Bone Regeneration

Comparison of osteogenic capability of 3D-printed bioceramic scaffolds and granules with different porosities for clinical translation

Superiority of Triply Periodic Minimal Surface Gyroid Structure to Strut-Based Grid Structure in Both Strength and Bone Regeneration

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