Gegen Qinlian decoction ameliorates murine colitis by inhibiting the expansion of Enterobacteriaceae through activating PPAR-γ signaling

Hu, Jingyi; Tong, Yiheng; Zheng, Shen; Li, Yanan; Cheng, Cheng; Au, Ryan; Xu, Feng; Liu, Yajun; Zhu, Lei; Shen, Hong

doi:10.1016/j.biopha.2022.113571

Cited by 12 publications

(3 citation statements)

References 26 publications

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“…The heatmap visualizes the overall gut flora of each group, and the changes in the abundance of intestinal flora reflect the effect of drugs on specific intestinal flora ( Cheng et al, 2023 ). AESc upregulated the abundance of beneficial Alcaligenaceae ( Collins et al, 2014 ), Coriobacteriaceae ( Sun et al, 2023 ), Ruminococcaceae ( Keshteli et al, 2022 ), Lachnospiraceae ( Herp et al, 2019 ), [Paraprevotellaceae], Mucispirillum ( Herp et al, 2019 ), Adlercreutzia ( Chen et al, 2019 ), Allobaculum ( van Muijlwijk et al, 2021 ), [ Ruminococcus ] ( Lee et al, 2017 ), and [ Prevotella ] ( Iljazovic et al, 2021 ) and downregulated the abundance of pathogenic Turicibacteraceae ( Tong et al, 2021 ), Enterobacteriaceae ( Hu et al, 2022 ), Proteus ( Zhang et al, 2021 ), Turicibacter ( Meng et al, 2019 ), Coprococcus ( Yang et al, 2023 ), Shigella ( Dekker and Frank, 2015 ), and Prevotella ( Sharma et al, 2022 ). However, the modulating effects on Desulfovibrionaceae ( Liu et al, 2023 ), Deferribacteraceae ( Berry et al, 2012 ), Helicobacteraceae ( Tong et al, 2021 ), Verrucomicrobiaceae ( Tong et al, 2021 ), Bacteroidaceae ( Peng et al, 2019 ), Lactobacillaceae ( Ma L. et al, 2022 ), Prevotellaceae ( Liu et al, 2020 ), Oscillospira ( Chen et al, 2021 ), Akkermansia ( Qu et al, 2021 ), Bacteroides ( Shao et al, 2021 ), and Lactobacillus ( Jia et al, 2022 ) were inconsistent with the literature reports.…”

Section: Discussionmentioning

confidence: 99%

Aqueous extract of Sargentodoxa cuneata alleviates ulcerative colitis and its associated liver injuries in mice through the modulation of intestinal flora and related metabolites

Xu,

Yu,

et al. 2024

Front. Microbiol.

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BackgroundUlcerative colitis (UC) is a refractory disease worldwide. Liver injury can be found clinically with UC, and now, it is found that gut dysbiosis is an important mechanism in the pathogenesis of UC. Sargentodoxa cuneata has been used as a traditional Chinese medicine and is commonly used clinically for the treatment of UC. The main objective of this study was to investigate the intrinsic mechanisms of Sargentodoxa cuneata in the treatment of UC and its associated liver injuries from the perspective of intestinal flora and related metabolites.MethodsUltra-performance liquid chromatography-mass spectrometry was used to identify the components in the aqueous extract of Sargentodoxa cuneata (AESc). Mice with UC induced by dextran sulfate sodium were used to study the effects of AESc on UC and its associated liver injuries. Furthermore, 16S rRNA gene sequencing and analysis were performed on intestinal contents, and correlation analysis of intestinal flora with short-chain fatty acids (SCFAs) and organic acids was performed.ResultsA total of 114 compounds were identified in AESc. AESc improved disease activity index scores, liver index, and colon length in mice with UC and had a good protective effect on intestine and liver injuries. Moreover, the administration of AESc regulated gut microbiota dysbiosis and the levels of a few SCFAs and organic acids in mice with UC. In addition, the correlation analysis results showed that the Megamonas and Bifidobacterium were the key intestinal flora related to the levels of differential SCFAs and organic acids in mice with UC after AESc intervention.ConclusionAESc has a good protective effect on UC and UC related liver injuries. Modulation of the intestinal flora and its metabolites (SCFAs and a few organic acids) is an important pathway for AESc in the treatment of UC and also provides a rationale for the clinical use of Sargentodoxa cuneata in the treatment of UC.

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Section: Discussionmentioning

confidence: 99%

Aqueous extract of Sargentodoxa cuneata alleviates ulcerative colitis and its associated liver injuries in mice through the modulation of intestinal flora and related metabolites

Xu,

Yu,

et al. 2024

Front. Microbiol.

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“…59,60 Additionally, XOSs modified both the PPAR and NF-kappa B signaling pathways, both being known to play a role in preserving intestinal inflammation and health. 61 Hu et al 62 discovered that Gegen Qinlian might alleviate colitis in mice by activating the PPAR signaling pathway to restore the intestinal barrier. Moreover, the NF-kappa B pathway was found to be a key regulator of cytokine production, closely related to inflammatory bowel disease pathophysiology.…”

Section: Discussionmentioning

confidence: 99%

Integrated metabolomics and transcriptomics revealed the anti-constipation mechanisms of xylooligosaccharides from corn cobs

Song,

Guo,

Sun

et al. 2024

Food Funct.

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“…The tags were then compared with the Silva database using the UCHIME Algorithm to remove chimera sequences and obtain effective tags. The QllME software was used to process the sequences and microbial composition analysis ( Hu et al, 2022 ; Zhang et al, 2022 ).…”

Section: Methodsmentioning

confidence: 99%

Tongbian decoction restores intestinal microbiota and activates 5-hydroxytryptamine signaling: implication in slow transit constipation

Li,

Lv,

et al. 2024

Front. Microbiol.

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IntroductionSlow transit constipation (STC) is a type of functional constipation. The detailed mechanism of STC, for which there is currently no effective treatment, is unknown as of yet. Tongbian decoction (TBD), a traditional Chinese medicinal formula, is commonly used to treat STC in clinical settings. However, the potential impact of TBD on the management of STC via modulation of the gut microbiota remains unclear.MethodsPseudo-germ-free rats were constructed after 6 days of treatment with bacitracin, neomycin, and streptomycin (abbreviated as ABX forthwith). Based on the successful construction of pseudo-germ-free rats, the STC model (ABX + STC) was induced using loperamide hydrochloride. After successful modeling, based on the different sources of donor rat microbiota, the ABX + STC rats were randomly divided into three groups: Control → ABX + STC, STC → ABX + STC, and STC + TBD → ABX + STC for fecal microbiota transplant (FMT). Body weight, fecal water content, and charcoal power propelling rate of the rats were recorded. Intestinal microbiota was detected by 16S rRNA sequencing, and the 5-hydroxytryptamine (5-HT) signaling pathway was examined by western blots, immunofluorescence, and immunohistochemical analysis.ResultsAfter treatment with fecal bacterial solutions derived from rats treated with Tongbian decoction (TBD), there was an increase in body weight, fecal water content, and the rate of charcoal propulsion in the rats. Additionally, activation of the 5-hydroxytryptamine (5-HT) signaling pathway was observed. The 16S rRNA sequencing results showed that the fecal bacterial solution from TBD-treated rats affected the intestinal microbiota of STC rats by increasing the proliferation of beneficial bacteria and suppressing the expansion of harmful bacteria.ConclusionOur study showed that TBD alleviated constipation in STC rats by modulating the structure of the intestinal microbiota.

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Gegen Qinlian decoction ameliorates murine colitis by inhibiting the expansion of Enterobacteriaceae through activating PPAR-γ signaling

Cited by 12 publications

References 26 publications

Aqueous extract of Sargentodoxa cuneata alleviates ulcerative colitis and its associated liver injuries in mice through the modulation of intestinal flora and related metabolites

Aqueous extract of Sargentodoxa cuneata alleviates ulcerative colitis and its associated liver injuries in mice through the modulation of intestinal flora and related metabolites

Integrated metabolomics and transcriptomics revealed the anti-constipation mechanisms of xylooligosaccharides from corn cobs

Tongbian decoction restores intestinal microbiota and activates 5-hydroxytryptamine signaling: implication in slow transit constipation

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