Gel filtration chromatography of fluorescent phenolic and heterocyclic compounds

Chromatography

“…Techniques and Apparatus. A new gradient elution chromatography (86) has dual columns, a 9-chamber solvent programmer, and UV and differential refractometer detectors in series. A splitter inlet system (396), an automatic apparatus for loading a number of samples at preselected times (435), and other devices (119,236) for automating column chromatography have been described.…”

“…Nitrophenols have been separated on polyamidetreated thin-layers (143) or on silica gel G-amylopectin (430). Phenolic and heterocyclic fluorescent compounds have been studied by gel filtration chromatography on Sephadex G-15 (86).…”

Trace elemental distribution in soil and compost-derived humic acid molecular fractions and colloidal organic matter in municipal wastewater by flow field-flow fractionation-inductively coupled plasma mass spectrometry (flow FFF-ICP-MS)

“…22 Recently Evans et al 21 separated humic and fulvic acids in aquatic media and successfully introduced gel micro-samples by laser ablation for subsequent off-line ICP-MS determination of lead bound to humic and fulvic acid fractions. However, gel-based methods exhibit artifacts resulting from solute adsorption and chargerepulsion effects 24,25 and perhaps shearing of very large macromolecules 26 in the solid phase.…”

Polymerization of pentachlorophenol and ferulic acid by fungal extracellular lignin-degrading enzymes

“…In control reactions (i.e., those without enzyme preparation, H 2 O 2 , or MnSO 4 ) the PCP was eluted as a broad peak around 150 ml, which was beyond the total effective column volume (Vt). Aromatic hydroxyl and methoxyl substituents are known to increase adsorption onto Sephadex (11). Therefore, elution of PCP in fractions beyond the Vt was probably due to interactions of the phenolic group with the Sephadex gel matrix.…”