Gel-free proteomics reveal potential biomarkers of priming-induced salt tolerance in durum wheat

Fercha, Azzedine; Capriotti, Anna Laura; Caruso, Giuseppe; Cavaliere, Chiara; Gherroucha, Hocine; Samperi, Roberto; Stampachiacchiere, Serena; Laganà, Aldo

doi:10.1016/j.jprot.2013.08.010

Cited by 64 publications

(44 citation statements)

References 59 publications

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“…Transcriptomic, proteomic, and metabolomic studies, including lipidomics, have shed some light on the importance of lipids in the response of plants to salt treatment, providing evidence that plant lipid metabolism is responsive to salinity at the gene, protein, and metabolite levels (Fercha et al, ; Kosová, Práil, & Vítámvás, ; Natera, Hill, Rupasinghe, & Roessner, ; Veeranagamallaiah et al, ). However, these studies have generally focused on salt‐sensitive plants.…”

Section: Introductionmentioning

confidence: 99%

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Single cell‐type analysis of cellular lipid remodelling in response to salinity in the epidermal bladder cells of the model halophyte Mesembryanthemum crystallinum

Barkla

Garibay-Hernández

Melzer

et al. 2018

Plant Cell & Environment

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Salt stress causes dramatic changes in the organization and dynamic properties of membranes, however, little is known about the underlying mechanisms involved. Modified trichomes, known as epidermal bladder cells (EBC), on the leaves and stems of the halophyte Mesembryanthemum crystallinum can be successfully exploited as a single-cell-type system to investigate salt-induced changes to cellular lipid composition. In this study, alterations in key molecular species from different lipid classes highlighted an increase in phospholipid species, particularly those from phosphatidylcholine and phosphatidic acid (PA), where the latter is central to the synthesis of membrane lipids. Triacylglycerol (TG) species decreased during salinity, while there was little change in plastidic galactolipids. EBC transcriptomic and proteomic data mining revealed changes in genes and proteins involved in lipid metabolism and the upregulation of transcripts for PIPKIB, PI5PII, PIPKIII, and phospholipase D delta suggested the induction of signalling processes mediated by phosphoinositides and PA. TEM and flow cytometry showed the dynamic nature of lipid droplets in these cells under salt stress. Altogether, this work indicates that the metabolism of TG might play an important role in EBC response to salinity as either an energy reserve for sodium accumulation and/or driving membrane biosynthesis for EBC expansion.

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Section: Introductionmentioning

confidence: 99%

“…lipid metabolism is responsive to salinity at the gene, protein, and metabolite levels (Fercha et al, 2013;Kosová, Práil, & Vítámvás, 2013;Natera, Hill, Rupasinghe, & Roessner, 2016;Veeranagamallaiah et al, 2008). However, these studies have generally focused on salt-sensitive plants.…”

mentioning

confidence: 99%

Single cell‐type analysis of cellular lipid remodelling in response to salinity in the epidermal bladder cells of the model halophyte Mesembryanthemum crystallinum

Barkla

Garibay-Hernández

Melzer

et al. 2018

Plant Cell & Environment

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“…Sequential extraction based on different solubility of protein classes in buffers is a method particularly suited [54][55][56][57][58][59][60][61][62]. A subcellular fractionation of tissue sample may be essential before extraction to simplify the protein identification as well as to retain their primary localization.…”

Section: Sample Preparationmentioning

confidence: 99%

“…Stress tolerance induced by priming is probably achieved through two strategies: seed priming sets in motion germination-related processes that facilitate the transition of quiescent dry seeds into germinating state and lead to improved potential of germination [58]. Successful rooting of plants largely depends on successful seed germination.…”

Section: Abiotic Stressmentioning

confidence: 99%

Food Proteins and Peptides

Samperi

Capriotti

Cavaliere

et al. 2015

Advanced Mass Spectrometry for Food Safety and Quality

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The qualitative and quantitative determination of proteins and peptides in raw or processed food is experiencing a growing interest and importance from both scientific and economic point of view. Proteomics and peptidomics are relatively new entries in the field of food security, safety and authenticity, and themselves can contribute to the emergence of new branches of the science of food, such as foodomics and the just born nutriomics, digestomics, and gut metagenomics/metaproteomics. Mass spectrometry, in combination with a wide variety of separation methods and bioinformatic tools, is the principal methodology for proteomics. Both the so-called “in-gel” and “gel-free shotgun” bottom-up approaches are widely used.\ud Among the arguments described in this chapter there are: stress effects on gene expression, postharvest (plant) and postmortem (livestock) protein modification, food safety, quality and authentication, food processing and quality control, frauds discovery, food peptidomics and digestomics

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“…The only metaproteomic paper already published was focused on ambient aerosol samples collected on the roof of the Max Planck Institute for Chemistry and exploited a gel‐based, time‐consuming proteomic approach which provided a very small number of protein identifications. Shotgun gel‐free separation is nowadays the best choice to analyze highly complex protein mixtures as it provides, compared to gel‐based approaches, a considerable reduction to the complexity of the sample without any loss of information . The focus of the current study was the development of a comprehensive metaproteomic platform for the determination of protein present in bioaerosol from work environments, for example, CP, WWTP, and agricultural holding (AH).…”

Section: Introductionmentioning

confidence: 99%

Development of an Analytical Method for the Metaproteomic Investigation of Bioaerosol from Work Environments

et al. 2019

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The metaproteomic analysis of air particulate matter provides valuable information about the properties of bioaerosols in the atmosphere and their influence on climate and public health. In this work, a new method for the extraction and analysis of proteins in airborne particulate matter from quartz microfiber filters is developed. Different protein extraction procedures are tested to select the best extraction protocol based on protein recovery. The optimized method is tested for the extraction of proteins from spores of ubiquitous bacteria species and used for the metaproteomic characterization of filters from three work environments. In particular, ambient aerosol samples are collected in a composting plant, in a wastewater treatment plant, and in an agricultural holding. A total of 179, 15, 205, and 444 proteins are identified in composting plant, wastewater treatment plant, and agricultural holding, (cow stable and blending plant), respectively. In agreement with the major categories of primary biological aerosol particles, all identified proteins originated primarily from fungi, bacteria, and plants. The paper is the first metaproteomic study applied to bioaerosol samples collected in occupationally relevant environmental sites and, even though not aimed at monitoring the risk exposure of workers, it provides information on the possible exposure in the working environmental sites.

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Gel-free proteomics reveal potential biomarkers of priming-induced salt tolerance in durum wheat

Cited by 64 publications

References 59 publications

Single cell‐type analysis of cellular lipid remodelling in response to salinity in the epidermal bladder cells of the model halophyte Mesembryanthemum crystallinum

Single cell‐type analysis of cellular lipid remodelling in response to salinity in the epidermal bladder cells of the model halophyte Mesembryanthemum crystallinum

Food Proteins and Peptides

Development of an Analytical Method for the Metaproteomic Investigation of Bioaerosol from Work Environments

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