Gelatin as a delivery vehicle for the controlled release of bioactive molecules

Young, Simon; Wong, Mark E.; Tabata, Yasuhiko; Mikos, Antonios G.

doi:10.1016/j.jconrel.2005.09.023

Cited by 971 publications

(665 citation statements)

References 57 publications

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“…Sustained release of BMPs can accelerate bone healing in osteoporosis [3,23]. GMs have been widely used as a controlledrelease carrier of growth factors with good biocompatibility and degradability [12,33]. Thus, we asked whether (1) controlled release of rhBMP-2 could be improved in a composite bone substitute with rhBMP-2-loaded GMs and CPC and (2) osteoporotic bone healing could be accelerated by increasing factors released from the composite bone substitute.…”

Section: Discussionmentioning

confidence: 99%

“…GMs are biodegradable, biocompatible, and can be crosslinked to increase their thermal and mechanical stability under physiologic conditions [33]. Degradation properties of GMs can be adjusted by varying the cross-linking agent concentration and reaction period [12,33]. Gelatinenriched CPC also shows strengthened mechanical properties and enhances the bioactivity of osteoblasts [1,2].…”

Section: Introductionmentioning

confidence: 99%

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Calcium Phosphate Cement with BMP-2-loaded Gelatin Microspheres Enhances Bone Healing in Osteoporosis: A Pilot Study

Liu

et al. 2010

Clinical Orthopaedics &Amp; Related Research

109

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Background The capacity for bone healing reportedly is limited in osteoporosis with a less than ideal environment for healing of bone grafts. We therefore developed a composite bone substitute with rhBMP-2 loaded gelatin microsphere (GM) and calcium phosphate cement (CPC) to use in osteoporosis. Questions/purposes We asked whether (1) controlled release of rhBMP-2 could be improved in this composite bone substitute and (2) increasing factors released from the bone substitute could accelerate osteoporotic bone healing. Methods We soaked rhBMP-2/GM/CPC and rhBMP-2/ CPC composites in simulated body fluid for 28 days and then determined the amount of rhBMP-2 released. Both composites were implanted in bone defects of osteoporotic goats and left in place for 45 and 140 days; the specimens then were evaluated mechanically (pushout test) and morphologically (CT scanning, histology). Results The in vitro study showed the new composite released more rhBMP-2 compared with rhBMP-2/CPC. CT showed the defects healed more quickly with new grafts. The bone mineralization rate was greater in rhBMP-2/GM/ CPC than in rhBMP-2/CPC after 45 days of implantation and the pushout test was stronger after 45 and 140 days of implantation. Conclusions The new graft composite released more loaded factors and appeared to repair osteoporotic bone defects. Clinical Relevance These preliminary data suggest the new composite can be used as a bone substitute to accelerate healing of fractures and bone defects in osteoporosis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Calcium Phosphate Cement with BMP-2-loaded Gelatin Microspheres Enhances Bone Healing in Osteoporosis: A Pilot Study

Liu

et al. 2010

Clinical Orthopaedics &Amp; Related Research

109

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“…Various natural and synthetic matrices have been investigated as carriers for bFGF. Natural materials include gelatin [31,32], collagen [33], fibrin [34], heparin [35] and alginate. [36] Synthetic matrices have been generated from poly(ethylene glycol)-g-poly(lactic acid) [37], poly(lactic acid) [38], poly(lactic-co-glycolic acid) [39,40], and methylidene malonate polymers.…”

Section: Discussionmentioning

confidence: 99%

Biodegradable elastomeric scaffolds with basic fibroblast growth factor release

Guan

Stankus

Wagner

2007

Journal of Controlled Release

149

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Scaffolds that better approximate the mechanical properties of cardiovascular and other soft tissues might provide a more appropriate mechanical environment for tissue development or healing in vivo. An ability to induce local angiogenesis by controlled release of an angiogenic factor, such as basic fibroblast growth factor (bFGF), from a biodegradable scaffold with mechanical properties more closely approximating soft tissue could find application in a variety of settings. Toward this end biodegradable poly(ester urethane)urea (PEUU) scaffolds loaded with bFGF were fabricated by thermally induced phase separation. Scaffold morphology, mechanical properties, release kinetics, hydrolytic degradation and bioactivity of the released bFGF were assessed. The scaffolds had interconnected pores with porosities of 90% or greater and pore sizes ranging from 34-173 μm. Scaffolds had tensile strengths of 0.25-2.8 MPa and elongations at break of 81-443%. Incorporation of heparin into the scaffold increased the initial burst release of bFGF, while the initial bFGF loading content did not change release kinetics significantly. The released bFGF remained bioactive over 21 days as assessed by smooth muscle mitogenicity. Scaffolds loaded with bFGF showed slightly higher degradation rates than unloaded control scaffolds. Smooth muscle cells seeded into the scaffolds with bFGF showed higher cell densities than for control scaffolds after 7 days of culture. The bFGFreleasing PEUU scaffolds thus exhibited a combination of mechanical properties and bioactivity that might be attractive for use in cardiovascular and other soft tissue applications.

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“…Hydrogels have often been used in drug delivery and controlled release because the release of polymeric active ingredients can be controlled by designing; hydrogel porosity (through the combination of polymer volume fraction and cross-link density) and/or hydrogel-active affinity (39)(40)(41)(42)(43)(44). In food natural (and modified) biopolymers such as polysaccharides (e.g.…”

Section: Introductionmentioning

confidence: 99%

Impact of Protein Gel Porosity on the Digestion of Lipid Emulsions

Sarkar

Juan

Kolodziejczyk

et al. 2015

J. Agric. Food Chem.

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Abstract:The present study sought to understand how the structure of protein gels impacts lipolysis of gelled emulsions. The selected system consisted of an o/w emulsion embedded within gelatine gels. The gelatine gelled emulsions consisted of a discontinuous network of aggregated emulsion droplets, dispersed within a continuous network of gelatine. The rheology of the gelled emulsions was dominated by the rheological behaviour of the gelatine, at no point was yielding or brittle fracture observed suggesting a gelatine continuous structure rather than a bi-continuous gel. A direct relationship between the speed of fat digestion and gel average mesh size was found, indicating that the digestion of fat within gelatine gelled emulsions is controlled by the gels ability to slow lipase diffusion to the interface of fat droplets. Digestion of fat was facilitated by deposition of the gelatine network, which mainly occurred via surface erosion catalysed by proteases. Overall this work has demonstrated that protein gels can considerably slow the kinetics of lipolysis of gelled emulsions, key for the future development of structures to control fat digestion and or the delivery of nutrients to different parts of the gastrointestinal tract.Introduction:

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Gelatin as a delivery vehicle for the controlled release of bioactive molecules

Cited by 971 publications

References 57 publications

Calcium Phosphate Cement with BMP-2-loaded Gelatin Microspheres Enhances Bone Healing in Osteoporosis: A Pilot Study

Calcium Phosphate Cement with BMP-2-loaded Gelatin Microspheres Enhances Bone Healing in Osteoporosis: A Pilot Study

Biodegradable elastomeric scaffolds with basic fibroblast growth factor release

Impact of Protein Gel Porosity on the Digestion of Lipid Emulsions

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