Gelatin-based nanoparticles as drug and gene delivery systems: Reviewing three decades of research

Elzoghby, Ahmed O.

doi:10.1016/j.jconrel.2013.09.019

Cited by 560 publications

(368 citation statements)

References 135 publications

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“…21 Briefly, 20 mg of the synthesized amphiphilic copolymer was dissolved in 1 mL of acetone (including 200 µg of coumarin 6) with the help of ultrasonication. The formed organic solution of polymers and fluorescence dye molecules was slowly added dropwise into 10 mL of Milli Q water, with constant stirring (700 rpm) at room temperature to prepare an emulsion, which was then stirred (300 rpm) in a fume hood at room temperature for 4 hours to completely remove the acetone.…”

Section: Micelle Formationmentioning

confidence: 99%

Effects of X-shaped reduction-sensitive amphiphilic block copolymer on drug delivery

Xiao¹,

Wang²

2015

IJN

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To study the effects of X-shaped amphiphilic block copolymers on delivery of docetaxel (DTX) and the reduction-sensitive property on drug release, a novel reduction-sensitive amphiphilic copolymer, (PLGA) 2 -SS-4-arm-PEG 2000 with a Gemini-like X-shape, was successfully synthesized. The formation of nanomicelles was proved with respect to the blue shift of the emission fluorescence as well as the fluorescent intensity increase of coumarin 6-loaded particles. The X-shaped polymers exhibited a smaller critical micelle concentration value and possessed higher micellar stability in comparison with those of linear ones. The size of X-shaped (PLGA) 2 -SS-4-arm-PEG 2000 polymer nanomicelles (XNMs) was much smaller than that of nanomicelles prepared with linear polymers. The reduction sensitivity of polymers was confirmed by the increase of micellar sizes as well as the in vitro drug release profile of DTX-loaded XNMs (DTX/XNMs). Cytotoxicity assays in vitro revealed that the blank XNMs were nontoxic against A2780 cells up to a concentration of 50 µg/mL, displaying good biocompatibility. DTX/XNMs were more toxic against A2780 cells than other formulations in both dose- and time-dependent manners. Cellular uptake assay displayed a higher intracellular drug delivery efficiency of XNMs than that of nanomicelles prepared with linear polymers. Besides, the promotion of tubulin polymerization induced by DTX was visualized by immunofluorescence analysis, and the acceleration of apoptotic process against A2780 cells was also imaged using a fluorescent staining method. Therefore, this X-shaped reduction-sensitive (PLGA) 2 -SS-4-arm-PEG 2000 copolymer could effectively improve the micellar stability and significantly enhance the therapeutic efficacy of DTX by increasing the cellular uptake and selectively accelerating the drug release inside cancer cells.

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Section: Micelle Formationmentioning

confidence: 99%

Effects of X-shaped reduction-sensitive amphiphilic block copolymer on drug delivery

Xiao¹,

Wang²

2015

IJN

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“…Biodegradable polymers resembling polysaccharides as well as proteins are mechanized the nanoparticles that can behave as proficient, controlled and targeted drug delivery fomite which has the endeavor to make the therapeutic effects healthier. For few decades, it has been the main trepidation to develop drug delivery devices as proteinsbased nanoparticles that are generally regarded as safe [9].…”

Section: A Rt I C L E I N F O Abstractmentioning

confidence: 99%

Liposome as nanocarrier: Site targeted delivery in lung cancer

Ullah¹,

Noreen²,

Tehreem³

et al. 2017

APJTD

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“…A albumina sérica humana (HSA) é a proteína plasmática mais abundante, possuindo um peso molecular de 66,4 kDa e relativa estabilidade no ambiente (60 °C e pH 4-9), além de baixa imunogenicidade e toxicidade negligenciável. 126,127 O atelocolágeno é o colágeno, principal componente da matriz extracelular, tratado com pepsina. A retirada de ateloproteínas diminui a antigenicidade do atelocolágeno, em relação ao colágeno.…”

Section: Proteínas Cationizadasunclassified

“…129 Esses polímeros, uma vez cationizados, são geralmente utilizados para a formação de complexos, hidrogéis e, para a via ocular, como nanopartículas preparadas por nanoprecipitação/geleificação iôni-ca. 130,131 Entre as características do polímero que determinam as suas propriedades como materiais para a terapia genética ocular estão peso molecular, número de grupamentos amino e forma da proteína. 127,132 O peso molecular influencia diretamente na toxicidade, onde pesos moleculares mais elevados estão associados a uma maior toxicidade em células HCE.…”

Section: Proteínas Cationizadasunclassified

“…130,131 Entre as características do polímero que determinam as suas propriedades como materiais para a terapia genética ocular estão peso molecular, número de grupamentos amino e forma da proteína. 127,132 O peso molecular influencia diretamente na toxicidade, onde pesos moleculares mais elevados estão associados a uma maior toxicidade em células HCE. 113,133 De maneira similar, um elevado número de grupos amino incrementa a toxicidade, porém também a eficácia de transfecção.…”

Section: Proteínas Cationizadasunclassified

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Biomateriais para formulações de base nanotecnológica visando terapia gênica ocular

et al. 2016

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Gelatin-based nanoparticles as drug and gene delivery systems: Reviewing three decades of research

Cited by 560 publications

References 135 publications

Effects of X-shaped reduction-sensitive amphiphilic block copolymer on drug delivery

Effects of X-shaped reduction-sensitive amphiphilic block copolymer on drug delivery

Liposome as nanocarrier: Site targeted delivery in lung cancer

Biomateriais para formulações de base nanotecnológica visando terapia gênica ocular

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