Gelatin/chitosan/hyaluronan scaffold integrated with PLGA microspheres for cartilage tissue engineering

Quitosana: biopolímero funcional com potencial industrial biomédico

“…112 Outra vantagem do uso da quitosana como arcabouço é que pode ser moldada em várias formas (membranas, blocos, tubos e microesferas) e possui uma excelente habilidade para formar estruturas porosas. 113 Várias composições têm sido utilizadas para produzir um arcabouço de quitosana que tenha um tamanho de poro e propriedades mecânicas desejáveis. 114 Uma das características mais promissoras da quitosana é sua excelente habilidade para ser processada em estruturas porosas para uso em transplante de células e regeneração tecidual.…”

Characterization of a Gelatin/Chitosan/Hyaluronan scaffold-polymer

“…Synthetic polymers such us poly(lactic acid) (PLA) and poly(glycolic acid) (PGA) have good mechanical properties and stability, but have poor cell-matrix interaction due to lack of appropriate informational structure for cell attachment, also, they can be toxic for the implanted cells as they undergo hydrolytic degradation producing lactic and glycolic acid (Chen et al 2002;Tan et al 2009). …”

“…These characteristics give it unique physicochemical properties as well as distinctive biological functions. As a consequence, HA and its derivatives have been widely investigated as materials for tissue engineering, for instance, the HA properties include many special interactions with growth factors, receptors and adhesion proteins that suggest that its incorporation into a chitosan-gelatin network it may modify the material bioactivity generating a hydrogel in which other matrix molecules can assemble (Mao et al 2003;Tan et al 2009). …”

“…Gelatin/Chitosan/Hyaluronan based scaffold-polymer has been combined to obtain a variety of biocompatible products for tissue engineering (Liu et al 2004;Tan et al 2009), but little information related to their physical properties is available. The biomaterial characterization is necessary in scaling-up from bench lab, pilot and industrial scale production.…”

“…They are also bioabsorbable allowing the generation of a new ECM by the implanted cells. It is therefore recognized the use of these biomaterials to develop scaffold for skin and cartilage regeneration (Liu et al 2004;Tan et al 2009). The favorable properties of these biopolymers are related to particular characteristics of its main components that in combination make the polymer a very versatile and functional scaffold for tissue engineering.…”