Gelatin methacrylate hydrogel with drug-loaded polymer microspheres as a new bioink for 3D bioprinting

Mirek, A.; Belaid, Habib; Bartkowiak, Aleksandra; Barranger, Fanny; Salmeron, Fanny; Kajdan, Marilyn; Grzeczkowicz, Marcin; Cavaillès, Vincent; Lewińska, Dorota; Bechelany, Mikhael

doi:10.1016/j.bioadv.2023.213436

Cited by 8 publications

(3 citation statements)

References 45 publications

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“…Equilibrium swelling ratio and marker substance release tests were conducted, demonstrating the influence of microspheres on the hydrogel's attributes. In addition, the bioink matrices were incorporated with ampicillin, and the release of antibiotics was governed, validating antibacterial action against S. aureus and Escherichia coli [89].…”

Section: • 3d Printing and 3d Bioprinting In Drug/cell Deliverymentioning

confidence: 99%

Unveiling the versatility of gelatin methacryloyl hydrogels: a comprehensive journey into biomedical applications

Pramanik,

Alhomrani,

Alamri

et al. 2024

Biomed. Mater.

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Gelatin methacryloyl (GelMA) hydrogels have gained significant recognition as versatile biomaterials in the biomedical domain. GelMA hydrogels emulate vital characteristics of the innate extracellular matrix (ECM) by integrating cell-adhering and matrix metalloproteinase-responsive peptide motifs. These features enable cellular proliferation and spreading within GelMA-based hydrogel scaffolds. Moreover, GelMA displays flexibility in processing, as it experiences crosslinking when exposed to light irradiation, supporting the development of hydrogels with adjustable mechanical characteristics. The drug delivery landscape has been reshaped by GelMA hydrogels, offering a favorable platform for the controlled and sustained release of therapeutic actives. The tunable physicochemical characteristics of GelMA enable precise modulation of the kinetics of drug release, ensuring optimal therapeutic effectiveness. In tissue engineering, GelMA hydrogels perform an essential role in the design of the scaffold, providing a biomimetic environment conducive to cell adhesion, proliferation, and differentiation. Incorporating GelMA in three-dimensional printing further improves its applicability in drug delivery and developing complicated tissue constructs with spatial precision. Wound healing applications showcase GelMA hydrogels as bioactive dressings, fostering a conducive microenvironment for tissue regeneration. The inherent biocompatibility and tunable mechanical characteristics of GelMA provide its efficiency in the closure of wounds and tissue repair.GelMA hydrogels stand at the forefront of biomedical innovation, offering a versatile platform for addressing diverse challenges in drug delivery, tissue engineering, and wound healing. This review provides a comprehensive overview, fostering an in-depth understanding of GelMA hydrogel’s potential impact on progressing biomedical sciences.

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Section: • 3d Printing and 3d Bioprinting In Drug/cell Deliverymentioning

confidence: 99%

Unveiling the versatility of gelatin methacryloyl hydrogels: a comprehensive journey into biomedical applications

Pramanik,

Alhomrani,

Alamri

et al. 2024

Biomed. Mater.

View full text Add to dashboard Cite

show abstract

“…Rapid in situ polymerized hydrogels fabricated from gelipincrosslinked gelatin [34], amino-gelatin and catecholmodified hyaluronic acid [35], and a combination of carboxymethyl chitosan and hydroxyethyl starch [36] are a few examples. Rapidly forming hydrogels can be easily synthesized by polymers functionalized with glycidyl methacrylate (GMA) or methacrylic anhydride (MA) [37,38]. The methacrylation of gelatin, collagen, alginate, and hyaluronic acids [37,[39][40][41] is predominantly achieved through MA.…”

Section: Introductionmentioning

confidence: 99%

“…Rapidly forming hydrogels can be easily synthesized by polymers functionalized with glycidyl methacrylate (GMA) or methacrylic anhydride (MA) [37,38]. The methacrylation of gelatin, collagen, alginate, and hyaluronic acids [37,[39][40][41] is predominantly achieved through MA. In contrast, SF and EPL methacrylation commonly uses GMA [38,42] that modifies the amino side groups on the polypeptides [38,42].…”

Section: Introductionmentioning

confidence: 99%

Fabrication and in vitro evaluation of photo cross-linkable silk fibroin–epsilon-poly-L-lysine hydrogel for wound repair

Sundaran,

Kok,

Chang

2023

Biomed. Mater.

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An optimal wound-healing hydrogel requires effective antibacterial properties and a favorable cell adhesion and proliferation environment. Although Bombyx mori silk fibroin (SF) possesses inherent wound-healing properties, it lacks these essential qualities. This study aimed to fabricate a novel photo-polymerizable hydrogel by utilizing SF's wound-healing efficiency and the epsilon-poly-L-lysine (EPL) antimicrobial activity. The SF was modified with three different concentrations of glycidyl methacrylate (GMA) to obtain SF-GMA(L), SF-GMA(M), and SF-GMA(H). A methacrylated EPL (EPL-GMA) was also produced. Then, SF-GMA was mixed with EPL-GMA to produce photo-crosslinkable SF-GMA-EPL hydrogels. The SF-GMA(L)-EPL, SF-GMA(M)-EPL, and SF-GMA(H)-EPL hydrogels, fabricated with 20% EPL-GMA, demonstrated maximum antimicrobial activity and mammalian cell adhesion ability. The hydroxyl radical (•OH) scavenging efficiency of the hydrogels was tested and shown to be between 69% and 74%. These hydrogels also exhibited 60% efficiency in removing bacterial lipopolysaccharides. The water absorption ability of the hydrogels was consistent with the size of their internal pores. The hydrogels exhibited a slow degradation fashion, and their degradation products appeared cytocompatible. Finally, the elastomeric properties of the hydrogels were determined, and a storage modulus (G') of 300–600 Pa was demonstrated. In conclusion, the hydrogels created in this study possess excellent biological and physical properties to support wound healing.

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Hofmeister Ions‐Induced Thinning of Gelatin to Enhance 3D Printing Precision

Chee,

Koo,

Sim

et al. 2024

Adv Materials Technologies

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Hydrogel 3D printing holds immense potential in fields like personalized medicine, regenerative therapies, and organ creation, offering biocompatible structures similar to the extracellular matrix. Gelatin‐Methacryloyl (GelMA) emerges as a promising candidate, while its high viscosity poses a significant challenge, especially in vat photopolymerization‐based 3D printing. Here, a new approach is presented by using Hofmeister ionic effect to substantially reduce the viscosity of high‐content (up to 60%) Gelatin bioink at room temperature with enhanced mechanical performance of the printed structures. The thinning effect induced by chaotropic Hofmeister ions is investigated through complex viscosity analysis, optical rotation measurements, and sol–gel conversion studies. The thinning effect induced by chaotropic ions enables precise 3D printing of Gelatin hydrogel, achieving accuracy comparable to prints made with polymers. Furthermore, after polymerization, the cations of the chaotropic salt change their role to cross‐linkers, leading to stronger scaffolds that exhibit biocompatibility with robust cell attachment, proliferation, and suitability for cell growth. The combination facilitates the creation of customizable structures and high printing accuracy will promote the wide application of Gelatin in the development of patient‐specific implants, drug delivery systems, and tissue scaffolds, further improving medical treatment efficacy and personalized healthcare.

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Gelatin methacrylate hydrogel with drug-loaded polymer microspheres as a new bioink for 3D bioprinting

Cited by 8 publications

References 45 publications

Unveiling the versatility of gelatin methacryloyl hydrogels: a comprehensive journey into biomedical applications

Unveiling the versatility of gelatin methacryloyl hydrogels: a comprehensive journey into biomedical applications

Fabrication and in vitro evaluation of photo cross-linkable silk fibroin–epsilon-poly-L-lysine hydrogel for wound repair

Hofmeister Ions‐Induced Thinning of Gelatin to Enhance 3D Printing Precision

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