Gelatin methacryloyl granular scaffolds for localized mRNA delivery

Carvalho, Bruna Gregatti; Nakayama, Aya; Miwa, Hiromi; Han, Sang Won; de la Torre, Lucimara Gaziola; Di Carlo, Dino; Lee, Junmin; Kim, Han‐Jun; Khademhosseini, Ali; de Barros, Natan Roberto

doi:10.1002/agt2.464

Cited by 3 publications

(3 citation statements)

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“…Integration of hydrogels with plasmonic nanoparticles is of high interest toward creating “nanocomposites” for tissue engineering, photothermal platforms, and surface-enhanced Raman scattering (SERS) bioimaging and sensing. − Common hydrogels used for biological applications are based on polymers like collagen, gelatin, alginate, and hyaluronic acid. − Hydrogels composed of gelatin methacryloyl (GelMA) see widespread use in tissue and tumor mimics, , cell culture substrates or scaffolds, , biosensors, and therapeutic platforms, due to their excellent biocompatibility (the base component, gelatin, is a natural biopolymer) and the high degree of tunability of GelMA’s physical properties . The main approaches for the fabrication of hydrogel nanocomposites include: (i) mixing colloidal nanoparticles with the uncured hydrogel polymer ,,, and (ii) nanoparticle growth induced by ultraviolet (UV) light irradiation of the metal precursor. − With either of these strategies, nanoparticles are introduced throughout the entire gel, which may undesirably alter the polymer’s physical and chemical properties. ,, Regarding sensing applications, additional drawbacks may include a reduced visible–NIR transparency, as well as hindered diffusion of large/less hydrophilic analytes to particle-containing sites, which are important factors for applications where molecules, tissues, or seeded cells are to be analyzed by SERS ( i.e.…”

Section: Introductionmentioning

confidence: 99%

“… 34 − 36 Common hydrogels used for biological applications are based on polymers like collagen, gelatin, alginate, and hyaluronic acid. 37 − 41 Hydrogels composed of gelatin methacryloyl (GelMA) see widespread use in tissue and tumor mimics, 42 , 43 cell culture substrates or scaffolds, 44 , 45 biosensors, 46 and therapeutic platforms, 47 due to their excellent biocompatibility (the base component, gelatin, is a natural biopolymer) and the high degree of tunability of GelMA’s physical properties. 38 The main approaches for the fabrication of hydrogel nanocomposites include: (i) mixing colloidal nanoparticles with the uncured hydrogel polymer 34 , 35 , 48 , 49 and (ii) nanoparticle growth induced by ultraviolet (UV) light irradiation of the metal precursor.…”

Section: Introductionmentioning

confidence: 99%

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Growing Gold Nanostars on 3D Hydrogel Surfaces

Vinnacombe-Willson,

García-Astrain,

Troncoso-Afonso

et al. 2024

Chem. Mater.

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Nanocomposites comprising hydrogels and plasmonic nanoparticles are attractive materials for tissue engineering, bioimaging, and biosensing. These materials are usually fabricated by adding colloidal nanoparticles to the uncured polymer mixture and thus require time-consuming presynthesis, purification, and ligand-exchange steps. Herein, we introduce approaches for rapid synthesis of gold nanostars (AuNSt) in situ on hydrogel substrates, including those with complex three-dimensional (3D) features. These methods enable selective AuNSt growth at the surface of the substrate, and the growth conditions can be tuned to tailor the nanoparticle size and density (coverage). We additionally demonstrate proof-of-concept applications of these nanocomposites for SERS sensing and imaging. High surface coverage with AuNSt enabled 1−2 orders of magnitude higher SERS signals compared to plasmonic hydrogels loaded with premade colloids. Importantly, AuNSt can be prepared without the addition of any potentially cytotoxic surfactants, thereby ensuring a high biocompatibility. Overall, in situ growth becomes a versatile and straightforward approach for the fabrication of plasmonic biomaterials.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Growing Gold Nanostars on 3D Hydrogel Surfaces

Vinnacombe-Willson,

García-Astrain,

Troncoso-Afonso

et al. 2024

Chem. Mater.

View full text Add to dashboard Cite

show abstract

“…Consequently, recent years have seen an increasing emphasis on the research and development of synthetic or semisynthetic polymer-based hydrogels with large pore features and responsive characteristics. − The interconnected large pores in these materials create opportunities for cell migration, while their adaptable properties allow for the adjustment of local hydrogel physicochemical properties. This dual capability ensures the hydrogel’s overall mechanical stability while allowing for localized degradation and adjustment of the material in direct contact with cells, effectively meeting the essential conditions for cell growth and tissue formation.…”

Section: Introductionmentioning

confidence: 99%

Transforming Cell–Drug Interaction through Granular Hydrogel-Mediated Delivery of Polyplex Nanoparticles for Enhanced Safety and Extended Efficacy in Gene Therapy

Zhang,

Sun,

Heng

et al. 2024

ACS Appl. Mater. Interfaces

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The utilization of hydrogels for DNA/cationic polymer polyplex nanoparticle (polyplex) delivery has significantly advanced gene therapy in tissue regeneration and cancer treatment. However, persistent challenges related to the efficacy and safety of encapsulated polyplexes, stemming from issues such as aggregation, degradation, or difficulties in controlled release during or postintegration with hydrogel scaffolds, necessitate further exploration. Here, we introduce an injectable gene therapy gel achieved by incorporating concentrated polyplexes onto densely packed hydrogel microparticles (HMPs). Polyplexes, when uniformly adhered to the gene therapy gel through reversible electrostatic interactions, can detach from the HMP surface in a controlled manner, contrasting with free polyplexes, and thereby reducing dose-dependent toxicity during transfection. Additionally, the integration of RGD cell adhesion peptides enhances the scaffolding characteristics of the gel, facilitating cell adhesion, migration, and further minimizing toxicity during gene drug administration. Notably, despite the overall transfection efficiency showing average performance, utilizing confocal microscopy to meticulously observe and analyze the cellular states infiltrating into various depths of the gene therapy gel resulted in the groundbreaking discovery of significantly enhanced local transfection efficiency, with primary cell transfection approaching 80%. This phenomenon could be potentially attributed to the granular hydrogel-mediated delivery of polyplex nanoparticles, which revolutionizes the spatial and temporal distribution and thus the "encounter" mode between polyplexes and cells. Moreover, the gene therapy gel's intrinsic injectability and self-healing properties offer ease of administration, making it a highly promising candidate as a novel gene transfection gel dressing with significant potential across various fields, including regenerative medicine and innovative living materials.

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Self‐assembled α‐lactalbumin nanostructures: encapsulation and controlled release of bioactive molecules in gastrointestinal in vitro model

de Oliveira Bianchi,

Fabrino,

Quintão

et al. 2024

J Sci Food Agric

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BACKGROUNDImplementing encapsulation techniques is pivotal in safeguarding bioactive molecules against environmental conditions for drug delivery systems. Moreover, the food‐grade nanocarrier is a delivery system and food ingredient crucial in creating nutraceutical foods. Nano α‐lactalbumin has been shown to be a promissory nanocarrier for hydrophobic molecules. Furthermore, the nanoprotein can enhance the tecno‐functional properties of food such as foam and emulsion. The present study investigated the nanostructured α‐lactalbumin protein (nano α‐la) as a delivery and controlled release system for bioactive molecules in a gastric‐intestinal in vitro mimic system.RESULTSThe nano α‐la was synthesized by a low self‐assembly technique, changing the solution ionic strength by NaCl and obtaining nano α‐la 191.10 ± 21.33 nm and a spherical shape. The nano α‐la showed higher encapsulation efficiency and loading capacity for quercetin than riboflavin, a potential carrier for hydrophobic compounds. Thermal analysis of nano α‐la resulted in a ΔH of −1480 J g−1 for denaturation at 57.44 °C. The nanostructure formed by self‐assembly modifies the foam volume increment and stability. Also, differences between nano and native proteins in emulsion activity and stability were noticed. The release profile in vitro showed that the nano α‐la could not hold the molecules in gastric fluid. The Weibull and Korsmeyer‐Peppas model better fits the release profile behavior in the studied fluids.CONCLUSIONThe present study shows the possibility of nano α‐la as an alternative to molecule delivery systems and nutraceutical foods' formulation because of the high capacity to encapsulate hydrophobic molecules and the improvement of techno‐functional properties. However, the nanocarrier is not perfectly suitable for the sustainable delivery of molecules in the gastrointestinal fluid, demanding improvements in the nanocarrier. © 2024 Society of Chemical Industry.

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Gelatin methacryloyl granular scaffolds for localized mRNA delivery

Cited by 3 publications

References 63 publications

Growing Gold Nanostars on 3D Hydrogel Surfaces

Growing Gold Nanostars on 3D Hydrogel Surfaces

Transforming Cell–Drug Interaction through Granular Hydrogel-Mediated Delivery of Polyplex Nanoparticles for Enhanced Safety and Extended Efficacy in Gene Therapy

Self‐assembled α‐lactalbumin nanostructures: encapsulation and controlled release of bioactive molecules in gastrointestinal in vitro model

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