Gelation of konjac glucomannan by acetylmannan esterases from Aspergillus oryzae

Saito, Miho; Nakaya, Makoto; Kondo, Tatsuya; Nakazawa, Masami; Ueda, Mitsuhiro; Naganawa, Shogo; Hasegawa, Yoshinori; Sakamoto, Tatsuji

doi:10.1016/j.enzmictec.2022.110075

Cited by 4 publications

(1 citation statement)

References 37 publications

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“…Therefore, it would be helpful to modify KGM to alter its water absorption or other properties. There are some reports in the literature on KGM modification, including acetylation, methylation, and oxidation [6][7][8][9][10][11][12]. Cheng et al [13] prepared partially acid-hydrolyzed konjac glucomannan.…”

Section: Introductionmentioning

confidence: 99%

Thermal and Rheological Performances Evaluation of a Modified Biopolymer for Fracturing Fluid System

Wang

Hao³

et al. 2022

Molecules

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Developing an efficient fracturing fluid system is an enduring hot topic in the petrochemical industries, especially regarding the exploitation of limited oil. Biopolymers, especially polysaccharides (e.g., konjac gum, guar gum), are widely applied as fracturing fluids in fracturing as a result of their advantages. Herein, we propose an easy method of modifying konjac gum (KGM) using isopropanol, sodium hydroxide, and chloroacetic acid to obtain modified konjac glum (MKGM). The MKGM and KGM gels were also obtained by using the self-prepared organic titanium high-temperature stabilizer and organic borate cross-linker. The prepared MKGM was characterized by multiscale techniques, including attenuated total reflection Fourier transform infrared (ATR-FTIR), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), differential scanning calorimetry (DSC), and rheology properties. The ATR-FTIR results showed that the etherification modification reaction occurred as designed. The XRD results showed that the regularity of KGM was destroyed after modification. The TGA and DSC results showed that the thermal stability improved. Rheology measurements illustrated that the temperature and shear resistance of MKGM were better than those of KGM. The MKGM gel could be applied in fracturing fluid systems at a lower frequency through viscoelastic measurements.

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Section: Introductionmentioning

confidence: 99%

Thermal and Rheological Performances Evaluation of a Modified Biopolymer for Fracturing Fluid System

Wang

Hao³

et al. 2022

Molecules

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Towards an understanding of the enzymatic degradation of complex plant mannan structures

Mafa,

Malgas

2023

World J Microbiol Biotechnol

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Plant cell walls are composed of a heterogeneous mixture of polysaccharides that require several different enzymes to degrade. These enzymes are important for a variety of biotechnological processes, from biofuel production to food processing. Several classical mannanolytic enzyme functions of glycoside hydrolases (GH), such as β-mannanase, β-mannosidase and α-galactosidase activities, are helpful for efficient mannan hydrolysis. In this light, we bring three enzymes into the model of mannan degradation that have received little or no attention. By linking their three-dimensional structures and substrate specificities, we have predicted the interactions and cooperativity of these novel enzymes with classical mannanolytic enzymes for efficient mannan hydrolysis. The novel exo-β-1,4-mannobiohydrolases are indispensable for the production of mannobiose from the terminal ends of mannans, this product being the preferred product for short-chain mannooligosaccharides (MOS)-specific β-mannosidases. Second, the side-chain cleaving enzymes, acetyl mannan esterases (AcME), remove acetyl decorations on mannan that would have hindered backbone cleaving enzymes, while the backbone cleaving enzymes liberate MOS, which are preferred substrates of the debranching and sidechain cleaving enzymes. The nonhydrolytic expansins and swollenins disrupt the crystalline regions of the biomass, improving their accessibility for AcME and GH activities. Finally, lytic polysaccharide monooxygenases have also been implicated in promoting the degradation of lignocellulosic biomass or mannan degradation by classical mannanolytic enzymes, possibly by disrupting adsorbed mannan residues. Modelling effective enzymatic mannan degradation has implications for improving the saccharification of biomass for the synthesis of value-added and upcycling of lignocellulosic wastes.

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The role of CE16 exo-deacetylases in hemicellulolytic enzyme mixtures revealed by the biochemical and structural study of the novel TtCE16B esterase

Pentari,

Zerva,

Kosinas

et al. 2024

Carbohydrate Polymers

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Gelation of konjac glucomannan by acetylmannan esterases from Aspergillus oryzae

Cited by 4 publications

References 37 publications

Thermal and Rheological Performances Evaluation of a Modified Biopolymer for Fracturing Fluid System

Thermal and Rheological Performances Evaluation of a Modified Biopolymer for Fracturing Fluid System

Towards an understanding of the enzymatic degradation of complex plant mannan structures

The role of CE16 exo-deacetylases in hemicellulolytic enzyme mixtures revealed by the biochemical and structural study of the novel TtCE16B esterase

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