Gelation of Uniform Interfacial Diffusant in Embedded 3D Printing

Shin, Sung-Chul; Brunel, Lucia G.; Cai, Betty; Kilian, David; Roth, Julien G.; Seymour, Alexis J.; Heilshorn, Sarah C.

doi:10.1002/adfm.202307435

Cited by 12 publications

(14 citation statements)

References 59 publications

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Section: Strategies To Characterize Diffusion In Bioprintingmentioning

confidence: 99%

“…Altogether, GUIDE-3DP enabled the fabrication of complex perfusable networks based on commonly applied materials and equipment while enabling precise control over the channel dimensions. [87] As summarized in Table 3, perfusable structures can be generated by either coaxial extrusion or diffusion-based interfacial gelation using a variety of bioinks and diffusants. In coaxial extrusion strategies, a core-shell nozzle is necessary to form a hollow structure, whereas in diffusion-induced interfacial Free radicals produced by a laser beam diffused into the printed filament, crosslinking an outer shell of the filament.…”

Section: Diffusion-induced Interfacial Gelationmentioning

confidence: 99%

“…[12,101] For a thorough mechanistic understanding of diffusive effects, accurate measurements of the diffusional properties of printed constructs are crucial, although few bioprinting studies to date have incorporated this type of characterization. [87,102] The concentration of a diffusant is commonly determined using fluorescence-based techniques. In one example, the diffusion coefficient of a rhodamine-based dye in a crosslinked Pluronic F127-diacrylate hydrogel was measured by injecting the dye into 3D-printed microchannels.…”

Section: Experimental Approachesmentioning

confidence: 99%

“…The FRAP-calculated diffusion coefficient was validated by measuring the diffusion profile in situ and comparing with predicted diffusion profiles. [87] While direct fluorescence imaging is the most common method to determine diffusivity in bioprinting studies, other in situ approaches to measure diffusivity within hydrogels have been developed. Another fluorescence-based technique is fluorescence correlation spectroscopy (FCS), which measures the fluorescence intensity fluctuations induced by solute diffusion and quantifies the diffusion time based on an autocorrelation function.…”

Section: Experimental Approachesmentioning

confidence: 99%

“…Altogether, these models serve to provide increased control over bioprinting processes and reduce the trial and error required for print optimization. [87] In addition to predicting diffusion during the bioprinting process, computational modeling may be applied to predict time-dependent changes in diffusion within bioprinted hydrogels, such as due to material degradation. As demonstrated for a non-printed, protein-loaded, degradable 4-arm PEG acrylatedithiolglycolate hydrogel, Fickian diffusion during degradation can be modeled by considering how swelling and degradation affect the mesh size of the system.…”

Section: Computational Modelingmentioning

confidence: 99%

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Diffusion‐Based 3D Bioprinting Strategies

Cai,

Kilian,

Ramos Mejia

et al. 2023

Advanced Science

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Abstract3D bioprinting has enabled the fabrication of tissue‐mimetic constructs with freeform designs that include living cells. In the development of new bioprinting techniques, the controlled use of diffusion has become an emerging strategy to tailor the properties and geometry of printed constructs. Specifically, the diffusion of molecules with specialized functions, including crosslinkers, catalysts, growth factors, or viscosity‐modulating agents, across the interface of printed constructs will directly affect material properties such as microstructure, stiffness, and biochemistry, all of which can impact cell phenotype. For example, diffusion‐induced gelation is employed to generate constructs with multiple materials, dynamic mechanical properties, and perfusable geometries. In general, these diffusion‐based bioprinting strategies can be categorized into those based on inward diffusion (i.e., into the printed ink from the surrounding air, solution, or support bath), outward diffusion (i.e., from the printed ink into the surroundings), or diffusion within the printed construct (i.e., from one zone to another). This review provides an overview of recent advances in diffusion‐based bioprinting strategies, discusses emerging methods to characterize and predict diffusion in bioprinting, and highlights promising next steps in applying diffusion‐based strategies to overcome current limitations in biofabrication.

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Section: Strategies To Characterize Diffusion In Bioprintingmentioning

confidence: 99%

Section: Diffusion-induced Interfacial Gelationmentioning

confidence: 99%

Section: Experimental Approachesmentioning

confidence: 99%

Section: Experimental Approachesmentioning

confidence: 99%

Section: Computational Modelingmentioning

confidence: 99%

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Diffusion‐Based 3D Bioprinting Strategies

Cai,

Kilian,

Ramos Mejia

et al. 2023

Advanced Science

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Satellite‐Based On‐Orbit Printing of 3D Tumor Models

Mo,

Zhang,

Wang

et al. 2023

Advanced Materials

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Space three dimension (3D） bioprinting provides a precise and bionic tumor model for evaluating the compound effect of the space environment on tumors, thereby providing insight into the progress of the disease and potential treatments. However, space 3D bioprinting faces several challenges, including prelaunch uncertainty, possible liquid leakage, long‐term culture in space, automatic equipment control, data acquisition, and transmission. Here, a novel satellite‐based 3D bioprinting device with high structural strength, small volume, and low weight (<6 kg) is developed. A microgel‐based biphasic thermosensitive bioink and suspension medium that supports the on‐orbit printing and in situ culture of complex tumor models is developed. An intelligent control algorithm that enables the automatic control of 3D printing, autofocusing, fluorescence imaging, and data transfer back to the ground is developed. To the authors' knowledge, this is the first time that on‐orbit printing of tumor models is achieved in space with stable morphology and moderate viability via a satellite. It is found that 3D tumor models are more sensitive to antitumor drugs in space than on Earth. This study opens up a new avenue for 3D bioprinting in space and offers new possibilities for future research in space life science and medicine.

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All‐Aqueous Embedded 3D Printing for Freeform Fabrication of Biomimetic 3D Constructs

Deng,

Qi,

Meng

et al. 2024

Advanced Materials

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All‐aqueous embedded 3D printing, which involves extruding inks in an aqueous bath, has emerged as a transformative platform for the freeform fabrication of 3D constructs with precise control. The use of a supporting bath not only enables the printing of arbitrarily designed 3D constructs but also broadens ink selection for various soft matters, advancing the wide application of this technology. This review focuses on recent progress in the freeform preparation of 3D constructs using all‐aqueous embedded 3D printing. It begins by discussing the significance of ultralow interfacial tension in all‐liquid embedded printing and highlights the fundamental concepts and properties of all‐aqueous system. The review then introduces recent advances in all‐aqueous embedded 3D printing and clarifies the key factors affecting printing stability and shape fidelity, aiming to guide expansion and assessment of emerging printing systems used for various representative applications. Furthermore, it proposes the potential scope and applications of this technology, including in vitro models, cytomimetic microreactors, and soft ionic electronics. Finally, the review discusses the challenges facing current all‐aqueous embedded 3D printing and offers future perspectives on possible improvements and developments.

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Gelation of Uniform Interfacial Diffusant in Embedded 3D Printing

Cited by 12 publications

References 59 publications

Diffusion‐Based 3D Bioprinting Strategies

Diffusion‐Based 3D Bioprinting Strategies

Satellite‐Based On‐Orbit Printing of 3D Tumor Models

All‐Aqueous Embedded 3D Printing for Freeform Fabrication of Biomimetic 3D Constructs

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